Identification of quantitative trait loci controlling resistance to maize chlorotic dwarf virus

Abstract

Ineffective screening methods and low levels of disease resistance have hampered genetic analysis of maize (Zea mays L.) resistance to disease caused by maize chlorotic dwarf virus (MCDV). Progeny from a cross between the highly resistant maize inbred line Oh1VI and the susceptible inbred line Va35 were evaluated for MCDV symptoms after multiple virus inoculations, using the viral vector Graminella nigrifrons. Symptom severity scores from three rating dates were used to calculate area under the disease progress curve (AUDPC) scores for vein banding, leaf twist and tear, and whorl chlorosis. AUDPC scores for the F(2) population indicated that MCDV resistance was quantitatively inherited. Genotypic and phenotypic analyses of 314 F(2) individuals were compared using composite interval mapping (CIM) and analysis of variance. CIM identified two major quantitative trait loci (QTL) on chromosomes 3 and 10 and two minor QTL on chromosomes 4 and 6. Resistance was additive, with alleles from Oh1VI at the loci on chromosomes 3 and 10 contributing equally to resistance.

The Genetic Architecture of Disease Resistance in Maize: A Synthesis of Published Studies

“…Co-localization of the major virus resistance genes (Msv1, Mv1, Wsm1, Wsm2, Wsm3, and Mdm1) and viral dQTL were evident. Many of these viral dQTL, however, had very large effects, explaining between 25 to 50% of total variation, and might be better described as major genes (38,42,60,76). A recessive gene conferring resistance to southern corn leaf blight (SCLB), rhm, co-localized with SCLB dQTL on chromosome 6.…”

Control of Virus Diseases in Maize

“…10. In this region, Wsm3, which confers resistance to WSMV, colocalizes with QTLs that confer resistance to MDMV, MCDV, MRFV, MNeSV, MSV, and MSpV (Dintinger, Verger, et al, 2005;Jones et al, 2011Jones et al, , 2004McMullen et al, 1994;Pernet, Hoisington, Dintinger, et al, 1999;Pernet, Hoisington, Franco, et al, 1999;Wang et al, 2007;Zambrano, Jones, Brenner, et al, 2014;. This region of the maize genome also carries loci for bacterial and fungal resistance (Redinbaugh & Pratt, 2008), as well as putative R genes Xiao et al, 2007).…”

“…By taking the virus' transmission mode into account, high efficiency inoculation conditions can be developed (Louie & Anderson, 1993;Zambrano et al, 2013). This approach has been effective for characterizing resistance QTLs under both greenhouse and field conditions (Table 8.2) ( Jones et al, 2004;Kyetere et al, 1999;Welz et al, 1998;Zambrano, Jones, Brenner, et al, 2014).…”

“…The same region includes enhancers of resistance to MDMV and other potyviruses (De Souza et al, 2008;Ding et al, 2012;Dussle et al, 2000;Jones et al, 2011;McMullen et al, 1994;Stewart, Haque, Jones, & Redinbaugh, 2013;Wang, Zhang, Zhuang, Qin, & Zhang, 2007;Zambrano, Jones, Brenner, et al, 2014;Zhang et al, 2003). The region also contains QTLs that confer resistance to the waikavirus MCDV, the negative sense RNA rhabdovirus MMV, the ambisense RNA tenuivirus MSpV, the single-stranded DNA geminivirus MSV, and the ambisense RNA virus WoMV (Dintinger, Verger, et al, 2005;Jones et al, 2004;Marçon, Kaeppler, & Jensen, 1997;Marçon, Kaeppler, Jensen, Senior, & Stuber, 1999;Ming et al, 1997;Welz et al, 1998;Zambrano, Jones, Brenner, et al, 2014).…”

“…As with the cluster on chr. 3, QTLs conferring resistance to viruses with different genome and replication strategies are found in this cluster, including MFSV, MCDV, WMoV, MMV, and MSV ( Jones et al, 2004;Marçon et al, 1997Marçon et al, , 1999Pernet, Hoisington, Dintinger, et al, 1999;Zambrano, Jones, Brenner, et al, 2014).…”