Identification of quantitative trait loci and candidate genes for primary metabolite content in strawberry fruit

Vallarino, José G.; Pott, Delphine M.; Cruz-Rus, Eduardo; Miranda, L.; Medina-Mínguez, J.J.; Valpuesta, Victoriano; Fernie, Alisdair R.; Sánchez-Sevilla, José F.; Osorio, Sonia; Amaya, Iraida

doi:10.1038/s41438-018-0077-3

Cited by 29 publications

(31 citation statements)

References 73 publications

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“…FaDHAR and FaGMP collocated with the QTL detected only 1 year on LG VII-1. Recently, a transcriptomic analysis by RNA-seq in pools of progeny lines contrasting in ascorbate content derived from the same population identified differential expression of gene MANNOSE-6-PHOSPHATE ISOMERASE 1 ( FaM6PI1 ) while FaMIOX was not differentially expressed (Vallarino et al, 2019). The FaM6PI1 gene was also located within the confidence interval of the major QTL detected on LG V-I, and it is highly similar to the Arabidopsis PMI gene that encodes the first enzyme in the SW pathway (Maruta et al, 2008).…”

Section: Approaches To Increase Ascorbate In Fruitsmentioning

confidence: 99%

Vitamin C Content in Fruits: Biosynthesis and Regulation

et al. 2019

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Throughout evolution, a number of animals including humans have lost the ability to synthesize ascorbic acid (ascorbate, vitamin C), an essential molecule in the physiology of animals and plants. In addition to its main role as an antioxidant and cofactor in redox reactions, recent reports have shown an important role of ascorbate in the activation of epigenetic mechanisms controlling cell differentiation, dysregulation of which can lead to the development of certain types of cancer. Although fruits and vegetables constitute the main source of ascorbate in the human diet, rising its content has not been a major breeding goal, despite the large inter- and intraspecific variation in ascorbate content in fruit crops. Nowadays, there is an increasing interest to boost ascorbate content, not only to improve fruit quality but also to generate crops with elevated stress tolerance. Several attempts to increase ascorbate in fruits have achieved fairly good results but, in some cases, detrimental effects in fruit development also occur, likely due to the interaction between the biosynthesis of ascorbate and components of the cell wall. Plants synthesize ascorbate de novo mainly through the Smirnoff-Wheeler pathway, the dominant pathway in photosynthetic tissues. Two intermediates of the Smirnoff-Wheeler pathway, GDP-D-mannose and GDP-L-galactose, are also precursors of the non-cellulosic components of the plant cell wall. Therefore, a better understanding of ascorbate biosynthesis and regulation is essential for generation of improved fruits without developmental side effects. This is likely to involve a yet unknown tight regulation enabling plant growth and development, without impairing the cell redox state modulated by ascorbate pool. In certain fruits and developmental conditions, an alternative pathway from D-galacturonate might be also relevant. We here review the regulation of ascorbate synthesis, its close connection with the cell wall, as well as different strategies to increase its content in plants, with a special focus on fruits.

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Section: Approaches To Increase Ascorbate In Fruitsmentioning

confidence: 99%

Vitamin C Content in Fruits: Biosynthesis and Regulation

et al. 2019

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“…In order to breed for higher concentrations of healthrelated phytochemicals such as polyphenols in the cultivated strawberry, it is first essential to understand the inheritance of such compounds. Many quantitative trait loci (QTL) mapping studies have been undertaken to investigate various aspects of cultivated strawberry fruit quality, including the identification of QTL for total anthocyanin content 18 , overall sweetness, titratable acidity and ascorbic acid content 19 , and fruit primary metabolite content 20 . More recently, a study of a 'Delmarvel' × 'Selva' progeny identified many QTL similar to previous studies 21 , and QTL for fruit quality have been identified in 23 related F 1 progenies using a pedigree-based approach 22 .…”

Section: Introductionmentioning

confidence: 99%

Major-effect candidate genes identified in cultivated strawberry (Fragaria × ananassa Duch.) for ellagic acid deoxyhexoside and pelargonidin-3-O-malonylglucoside biosynthesis, key polyphenolic compounds

Davik¹,

Aaby²,

Buti³

et al. 2020

Hortic Res

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Strawberries are rich in polyphenols which impart health benefits when metabolized by the gut microbiome, including anti-inflammatory, neuroprotective, and antiproliferative effects. In addition, polyphenolic anthocyanins contribute to the attractive color of strawberry fruits. However, the genetic basis of polyphenol biosynthesis has not been extensively studied in strawberry. In this investigation, ripe fruits from three cultivated strawberry populations were characterized for polyphenol content using HPLC-DAD-MS n and genotyped using the iStraw35k array. GWAS and QTL analyses identified genetic loci controlling polyphenol biosynthesis. QTL were identified on four chromosomes for pelargonidin-3-O-malonylglucoside, pelargonidin-3-O-acetylglucoside, cinnamoyl glucose, and ellagic acid deoxyhexoside biosynthesis. Presence/absence of ellagic acid deoxyhexoside and pelargonidin-3-O-malonylglucoside was found to be under the control of major gene loci on LG1X2 and LG6b, respectively, on the F. × ananassa linkage maps. Interrogation of gene predictions in the F. vesca reference genome sequence identified a single candidate gene for ellagic acid deoxyhexoside biosynthesis, while seven malonyltransferase genes were identified as candidates for pelargonidin-3-O-malonylglucoside biosynthesis. Homologous malonyltransferase genes were identified in the F. × ananassa 'Camarosa' genome sequence but the candidate for ellagic acid deoxyhexoside biosynthesis was absent from the 'Camarosa' sequence. This study demonstrated that polyphenol biosynthesis in strawberry is, in some cases, under simple genetic control, supporting previous observations of the presence or absence of these compounds in strawberry fruits. It has also shed light on the mechanisms controlling polyphenol biosynthesis and enhanced the knowledge of these biosynthesis pathways in strawberry. The above findings will facilitate breeding for strawberries enriched in compounds with beneficial health effects.

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“…Evaluation and genetic mapping of both specialized (polyphenolics, flavor components) and primary (sugars, organic acids) metabolites are indispensable to identify the genetic architecture of fruit sensorial and nutritional quality in cultivated strawberry. (20,45–47). In addition, genetic studies allow pinpointing genotypes that can be used in a breeding scheme for the genetic improvement of strawberry.…”

Section: Discussionmentioning

confidence: 99%

“…(10), which are typically bi-parental populations in cultivated strawberry. (20,45–47). Using such a bi-parental population issued from a cross between two parents with contrasted sensory trait values (20) and by additionally breaking down the flavonoid composition to its individual chemical components (22), we were able to map mQTLs for each chemical compound from the three flavonoid chemical classes detected in strawberry fruit.…”

Section: Discussionmentioning

confidence: 99%

Metabolite Quantitative Trait Loci for flavonoids provide new insights into the genetic architecture of strawberry (Fragariaxananassa) fruit quality

Labadie

Vallin

Petit

et al. 2020

Preprint

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28Flavonoids are products from specialized metabolism that contribute to fruit sensorial (colour) and 29 nutritional (antioxidant properties) quality. Here, using a pseudo full-sibling F1 progeny previously 30 studied for fruit sensorial quality of cultivated strawberry (Fragaria x ananassa), we explored over two 31 successive years the genetic architecture of flavonoid-related traits using LC-ESI-MS (13 compounds 32 including anthocyanins, flavonols and flavan-3-ols) and colorimetric assays (anthocyanins, flavonoids, 33 phenolics, FRAP and TEAC antioxidant capacity). Network correlation analysis highlighted the high 34 connectivity of flavonoid compounds within each chemical class and low correlation with colorimetric 35 traits except anthocyanins. Mapping onto the female and male linkage maps of 152 flavonoid metabolic 36 QTLs (mQTLs) and of 26 colorimetric QTLs indicated co-localization on few linkage groups of major 37 flavonoid-and taste-related QTLs previously uncovered. These results pave the way for the discovery of 38 genetic variations underlying flavonoid mQTLs and for marker-assisted selection of strawberry varieties 39 with improved sensorial and nutritional quality.40 41 Keywords. Fragaria x ananassa, fruit, specialized metabolism, flavonoids, anthocyanins, mQTL, colour, 42 antioxidant, nutritional quality 43 44 93 by Marker-Assisted Selection (MAS) of the sensorial and nutritional quality of strawberry fruit.94 95 Material and methods 96 Plant materials and preparation. 97A pseudo full-sibling F1 population of 165 individuals obtained from a cross between the variety 98 'Capitola' (CA75.121-101 x Parker, University of California, Davis, USA) and the advanced line 'CF1116' 99 (['Pajaro' x ('Earlyglow' x 'Chandler')], reference from Ciref, France) was developed. The two parents, 100'Capitola' and 'CF1116', display many contrasting fruit quality traits. (20,26). For each of the two 101 consecutive study years (2010 and 2011), six cold-stored strawberry plants per genotype, which were 102 planted in 2009 and 2010, were grown in soil-free pine bark substrate under plastic tunnel with daily 103 ferti-irrigation and control of biotic stresses. Mapping population included a total of 165 individuals over 104 the two study years. 105Within this progeny, 72 and 131 individuals including parents were respectively phenotyped in 2010 106 and in 2011. Fruits were harvested at red ripe stage, when red coloration of the fruit is homogeneous. 107To ensure conformity of the fruit samples and to avoid undesirable effects on antioxidant and fruit 108 polyphenolic contents, several precautions were taken. Fruits were collected in the morning and those 109 showing abnormal shape and size, or injuries were not harvested. For each genotype, two harvests of 4-110 8 fruits each were performed at the peak of fruit production (8-16 fruits in total). Fruits were 111 immediately frozen in liquid nitrogen and stored at -80°C, in order to avoid degradation of antioxidants 112 and polyphenolics. They were then ground into a fine po...

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Identification of quantitative trait loci and candidate genes for primary metabolite content in strawberry fruit

Cited by 29 publications

References 73 publications

Vitamin C Content in Fruits: Biosynthesis and Regulation

Vitamin C Content in Fruits: Biosynthesis and Regulation

Major-effect candidate genes identified in cultivated strawberry (Fragaria × ananassa Duch.) for ellagic acid deoxyhexoside and pelargonidin-3-O-malonylglucoside biosynthesis, key polyphenolic compounds

Metabolite Quantitative Trait Loci for flavonoids provide new insights into the genetic architecture of strawberry (Fragariaxananassa) fruit quality

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