Identification of Quantitative Trait Loci (QTL) Underlying Protein, Oil, and Five Major Fatty Acids’ Contents in Soybean

Akond, Masum; Liu, Shiming; Boney, Melanie; Kantartzi, Stella K.; Meksem, Khalid; Bellaloui, Nacer; Lightfoot, David A.; Kassem, My Abdelmajid

doi:10.4236/ajps.2014.51021

Cited by 57 publications

(43 citation statements)

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“…The protein content in our wild soybean sample averaged about 48%, a higher mean percentage than typically is seen in cultivated soybeans [6, 17]. Consistent with this, Chen and Nelson [33] found that the mean protein level in a wild soybean sample was significantly higher than that in a sample of cultivated soybeans.…”

Section: Discussionsupporting

confidence: 77%

“…Selection for increased oil content has been practiced for many years, and Zhou et al [19] recently have identified 96 separate selective sweeps located within known oil QTL regions. Oil variability in our wild soybean sample as assessed by the coefficient of variation (10.8) was higher than comparable estimates for cultivated soybeans such as that of 4.74 calculated by Akond et al [6] among recombinant inbred lines.…”

Section: Discussioncontrasting

confidence: 76%

“…Fortunately, the mutant FAD2 genes discussed above do precisely this by largely disrupting the conversion of oleic acid precursors into linoleic acid precursors [47]. As a consequence, less emphasis appears to have been put on discovering major genes that reduce linoleic acid levels, although a number of QTLs with minor effects on fatty acid have been discovered [6, 18]. …”

Section: Discussionmentioning

confidence: 99%

“…Much of our genetic knowledge has come from discovery of quantitative trait loci (QTLs) located at various sites on all of the 20 chromosomes throughout the soybean genome that affect one or more of these traits [5, 6, 8–10]. Many of these QTLs were discovered through linkage mapping that requires F 2 , backcross, or recombinant inbred populations derived from original biparental crosses.…”

Section: Introductionmentioning

confidence: 99%

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A genome-wide association study of seed composition traits in wild soybean (Glycine soja)

et al. 2017

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BackgroundCultivated soybean (Glycine max) is a major agricultural crop that provides a crucial source of edible protein and oil. Decreased amounts of saturated palmitic acid and increased amounts of unsaturated oleic acid in soybean oil are considered optimal for human cardiovascular health and therefore there has considerable interest by breeders in discovering genes affecting the relative concentrations of these fatty acids. Using a genome-wide association (GWA) approach with nearly 30,000 single nucleotide polymorphisms (SNPs), we investigated the genetic basis of protein, oil and all five fatty acid levels in seeds from a sample of 570 wild soybeans (Glycine soja), the progenitor of domesticated soybean, to identify quantitative trait loci (QTLs) affecting these seed composition traits.ResultsWe discovered 29 SNPs located on ten different chromosomes that are significantly associated with the seven seed composition traits in our wild soybean sample. Eight SNPs co-localized with QTLs previously uncovered in linkage or association mapping studies conducted with cultivated soybean samples, while the remaining SNPs appeared to be in novel locations. Twenty-four of the SNPs significantly associated with fatty acid variation, with the majority located on chromosomes 14 (6 SNPs) and seven (8 SNPs). Two SNPs were common for two or more fatty acids, suggesting loci with pleiotropic effects. We also identified some candidate genes that are involved in fatty acid metabolism and regulation. For each of the seven traits, most of the SNPs produced differences between the average phenotypic values of the two homozygotes of about one-half standard deviation and contributed over 3% of their total variability.ConclusionsThis is the first GWA study conducted on seed composition traits solely in wild soybean populations, and a number of QTLs were found that have not been previously discovered. Some of these may be useful to breeders who select for increased protein/oil content or altered fatty acid ratios in the seeds. The results also provide additional insight into the genetic architecture of these traits in a large sample of wild soybean, and suggest some new candidate genes whose molecular effects on these traits need to be further studied.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3397-4) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 77%

Section: Discussioncontrasting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A genome-wide association study of seed composition traits in wild soybean (Glycine soja)

et al. 2017

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“…Therefore, in the current research, several QTLs were found that did not coincide with other researchers groups, but some QTLs were detected previously using the same population MD96-5722 x Spencer [23] [24]. For example, a linoleic acid QTL was detected by others elsewhere [7] on LG N (Chr3) at 4.80 cM position was very close to the QTL for ARG at 4.00 cM; the QTL we identified for SER on LG C2 (Chr6) has been identified for raffinose at the same position (7.20 cM) (unpublished data)and the QTL identified in LG K (Chr 9) for SER and TRP (0.50 cM) were very close to QTL identified in a previous study for the seed content of the isoflavone glycitein (0.60 cM; [20].…”

Section: Amino Acids Qtl Detectionmentioning

confidence: 89%

Mapping of QTL Associated with Seed Amino Acids Content in “MD96-5722” by “Spencer” RIL Population of Soybean Using SNP Markers

Khandaker¹,

Akond²,

Liu³

et al. 2015

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. (2015) Mapping of QTL Associated with Seed Amino Acids Content in "MD96-5722" by "Spencer" RIL Population of Soybean Using SNP Markers. AbstractLimited information is available on the genetic analysis of amino acid composition in soybean seeds. Previously, quantitative trait loci (QTLs) for seed isoflavones, protein, oil, and fatty acids were identified in the "MD96-5722" by "Spencer" and other RIL populations. There were wide variations for these seed constituents among the RIL populations. Therefore, the objective of this study was to identify QTLs controlling different amino acids content in soybean seeds. To achieve this objective, ninety-two F5:7 recombinant inbred lines (RIL), developed from a cross of MD96-5722 and Spencer, using a total 5376 Single Nucleotide Polymorphism (SNPs) markers, were used. The RILs were genotyped by using 537 polymorphic, reliably segregating SNP markers, developed from the Illumina Infinium SoySNP6K BeadChip array. A total of 13 QTLs were identified with three QTLs for threonine on the linkage group (LG) A1, C2, and B2. Two QTLs were identified for each of the amino acids proline on LG D1a and B2, serine on LG A1 and C2, tryptophan on LG K and G, and cysteine on LG A1 and K. One QTL was identified for arginine on LG N and histidine on LG J. The new QTLs findings for seed amino acid will facilitate the development of soybean cultivars with higher protein and amino acid quality to help meet the industry and consumer needs.* Corresponding author. L. Khandaker et al.975

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Quantitative trait loci governing seed protein, oil, and linolenic acid concentration in soybean

Moore Ronald,

Wyman,

Cunicelli

et al. 2024

J Americ Oil Chem Soc

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Soybean is one of the most economically important crops in the United States. Produced for its oil and protein concentration, it is readily utilized in food products for both human and livestock consumption. Since soybean was first cultivated in the United States, increased yield has been the driving factor in breeding efforts. Though yield and oil have been observed to be positively correlated, protein concentration is negatively correlated with both. An increased effort has been underway recently to produce high‐yielding cultivars that have both elevated oil and protein concentration. This has been accomplished utilizing molecular markers associated with quantitative trait loci (QTL) for both traits. To assist in this effort, more information on QTL associated with quality traits is required. In this study, 180 F4:6 recombinant inbred lines (RILs) segregating for protein, oil, and fatty acids were produced from a cross between TN12‐4098 and TN13‐4303. These lines were grown across three locations in Tennessee: Research and Education Center at Milan (RECM), Highland Rim Research and Education Center (HRREC), and East Tennessee Research and Education Center (ETREC) in 2018 and 2019. Sixteen QTL were found for protein, oil, linolenic acid, and meal protein concentration. Of these identified QTL, six were novel. Developing molecular markers associated with these QTL will assist in breeding efforts to produce high‐quality elite soybean cultivars that meet the demands of both farmers and consumers.

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Identification of Quantitative Trait Loci (QTL) Underlying Protein, Oil, and Five Major Fatty Acids’ Contents in Soybean

Abstract: LG-E (Chr_15), LG-J (Chr_16), and LG-G (Chr_18). The SNP markers closely linked to the QTL reported here will be useful for development of cultivars with altered oil and fatty acid compositions in soybean breeding programs.

Cited by 57 publications

References 35 publications

A genome-wide association study of seed composition traits in wild soybean (Glycine soja)

A genome-wide association study of seed composition traits in wild soybean (Glycine soja)

Mapping of QTL Associated with Seed Amino Acids Content in “MD96-5722” by “Spencer” RIL Population of Soybean Using SNP Markers

Quantitative trait loci governing seed protein, oil, and linolenic acid concentration in soybean

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