Identification of Quantitative Trait Loci Conferring Resistance to Bemisia tabaci in an F2 Population of Solanum lycopersicum × Solanum habrochaites Accession LA1777

Abstract

<jats:p><jats:italic>Solanum habrochaites</jats:italic> S. Knapp and D.M. Spooner accession LA1777 have reported resistance to the sweetpotato whitefly (SPWF), <jats:italic>Bemisia tabaci</jats:italic> (Genn.). An interspecific F<jats:sub>2</jats:sub> population of 171 plants between tomato [<jats:italic>Solanum lycopersicum</jats:italic> L. (formerly <jats:italic>Lycopersicon esculentum</jats:italic> Mill.)] and LA1777 was bioassayed against adult SPWF in a greenhouse using clip cages. A selective genotyping analysis was used with 11 resistant and 10 susceptible plants to locate resistance genes by testing them with molecular markers spanning most of the tomato genome at about 10-cM intervals. Markers in four regions were found to be associated with resistance, where three of them showed significantly strong associations and one showed a weak association through chi-square and analyses of variance. However, through quantitative trait locus (QTL) analysis using molecular markers, all four regions were identified as major QTLs with logarithm of odds (LOD) values of 4.87 to 5.95. The four QTLs were identified near the markers TG313 on chromosome 10, C2_At2g41680 on chromosome 9, TG523/T0408 on chromosome 11, and TG400/cLEG-37-G17 on chromosome 11. Multiple regression analysis produced similar results as above with fixed effects of single loci as well as interaction among some of the QTLs.</jats:p>

Exploiting natural variation to identify insect‐resistance genes

“…Well‐known examples include QTLs controlling the production of methylketones and acylsugars in wild relatives of tomato (Ben‐Israel et al. , 2009; Momotaz et al. , 2010).…”

“…Insect-resistance QTLs have been mapped in several plant species Hao and Lin Hong-Xuan, 2010). Well-known examples include QTLs controlling the production of methylketones and acylsugars in wild rela-tives of tomato (Ben-Israel et al, 2009;Momotaz et al, 2010). Furthermore, many researches have been performed on variation in the production of glucosinolates and the QTLs controlling this (Kliebenstein, 2009b).…”

Quantitative resistance against Bemisia tabaci in Solanum pennellii: Genetics and metabolomics

“…Without exception, all identified whitefly‐resistance QTLs were minor effect QTLs with low explained variances (Table ). Other QTL studies concerning tomato‐whitefly resistance traits on S. habrochaites also exclusively showed minor effect QTLs (Maliepaard et al ; Momotaz et al ). Leckie et al () showed that previously identified QTLs affecting acyl sugar concentration on Chromosomes IV and X also affected whitefly performance.…”

“…On S. habrochaites LA1777 four QTLs (on Chromosomes IX, X, and two on XI) were identified that were associated with resistance to B. tabaci (Momotaz et al ). However, none of these QTLs correspond to the regions in which we found whitefly‐resistance QTLs.…”

Role of QTL mapping to circumscribe various diseases in different crops with special emphasis on cotton.

“…On chromosome number 119 SNPs were identified in 82 to 93 Mb region which correspond the fine mapping of Msv1 [82]. Many other researchers also identified QTLs linked to MSV resistance [83,84] were conducted molecular mapping analysis in inbred line of maize which was multiple virus-resistant and studied six virus diseases resistance [85][86][87][88][89][90][91][92][93][94][95][96][97][98][99][100][101][102][103][104]. The inbred line of maize Oh1VI were used which is resistant to about ten different viruses.…”