Identification of QTLs for germination and seedling development in sunflower (Helianthus annuus L.)

Seed quality in tomato is associated with many complex physiological and genetic traits. While plant processes are frequently controlled by the action of small-to large-effect genes that follow classic Mendelian inheritance, our study suggests that seed quality is primarily quantitative and genetically complex. Using a recombinant inbred line population of Solanum lycopersicum ¥ Solanum pimpinellifolium, we identified quantitative trait loci (QTLs) influencing seed quality phenotypes under non-stress, as well as salt, osmotic, cold, high-temperature and oxidative stress conditions. In total, 42 seed quality traits were analysed and 120 QTLs were identified for germination traits under different conditions. Significant phenotypic correlations were observed between germination traits under optimal conditions, as well as under different stress conditions. In conclusion, one or more QTLs were identified for each trait with some of these QTLs co-locating. Co-location of QTLs for different traits can be an indication that a locus has pleiotropic effects on multiple traits due to a common mechanistic basis. However, several QTLs also dissected seed quality in its separate components, suggesting different physiological mechanisms and signalling pathways for different seed quality attributes.

show abstract

“…1999, 2003b), Arabidopsis (Quesada et al . 2002), and 1 QTL was also mapped in sunflower (Al‐Chaarani et al . 2005).…”

Section: Discussionmentioning

confidence: 99%

Complex genetics controls natural variation among seed quality phenotypes in a recombinant inbred population of an interspecific cross between Solanum lycopersicum × Solanum pimpinellifolium

Kazmi

Khan

Willems

et al. 2011

Plant Cell & Environment

View full text Add to dashboard Cite

show abstract

“…Because seed germination involves a series of cellular and metabolic events that are difficult to monitor directly (Bewley and Black 1994), defined germination traits can at best only indirectly mark physiological and biochemical progress of the process. The genetic basis of germination was addressed in sunflower, where four QTL affecting germination percentage were mapped to three linkage groups, six QTL affecting the percentage of normal seedlings to four linkage groups, and two QTL affecting germination timing to two linkage groups (Al-Chaarani et al 2005). In barley, germination QTL were mapped to chromosomes 2H, 3H, 5H, 6H and 7H (Edney and Mather 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Several such attempts have been made to study the inheritance of germination and seedling growth traits in Brassica ssp. (Bettey et al 2000), rice (Cui et al 2002), barley (Edney and Mather 2004), sunflower (Al Chaarani et al 2005) and wheat (Landjeva et al 2008). Genomic regions harbouring QTL associated with seed longevity have been identified in rice (Miura et al 2002;Sasaki et al 2005;Zeng et al 2006;Xue et al 2008), Arabidopsis thaliana (Clerkx et al 2004) and barley (Nagel et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Genetic mapping within the wheat D genome reveals QTL for germination, seed vigour and longevity, and early seedling growth

2009

View full text Add to dashboard Cite

Quantitative trait loci (QTL) controlling germination, seed vigour and longevity, and early seedling growth were identified using a set of common wheat lines carrying known D genome introgression segments. Seed germination (capacity, timing, rate and synchronicity) was characterized by a standard germination test, based either on the 1 mm root protrusion (germination sensu stricto) or the development of normal seedlings. To quantify seed vigour, the same traits were measured from batches of seed exposed for 72 h at 43°C and high (ca. 100%) humidity. Seed longevity was evaluated from the relative trait values. Seedling growth was assessed both under non-stressed and under osmotic stress conditions. Twenty QTL were mapped to chromosomes 1D, 2D, 4D, 5D, and 7D. Most of the QTL for germination sensu stricto clustered on chromosome 1DS in the region Xgwm1291-Xgwm337. A region on chromosome 7DS associated with Xgwm1002 harboured loci controlling the development of normal seedlings. Seed vigour-related QTL were present in a region of chromosome 5DL linked to Xgwm960. QTL for seed longevity were coincident with those for germination or seed vigour on chromosomes 1D or 5D. QTL for seedling growth were identified on chromosomes 4D and 5D. A candidate homologues search suggested the putative functions of the genes within the respective regions. These results offer perspectives for the selection of favourable alleles to improve certain vigour traits in wheat, although the negative effects of the same chromosome regions on other traits may limit their practical use.

show abstract

“…Based on these mutants, genetic approach has facilitated in obtaining knowledge regarding stratification regulating , lipid reserve mobilization , amino acid metabolism , DNA damage recovering , and epigenetic regulating mechanism . Furthermore, quantitative trait loci studies were performed in Arabidopsis and other species, including sunflower, Brassica oleracea , and Medicago truncatula , which detected several novel genes that participate in seed germination regulation . This technique is also applied in construction of genetic architecture of seed germination in response to environmental stresses .…”

Section: Introductionmentioning

confidence: 99%

Studies on the molecular mechanisms of seed germination

2015

View full text Add to dashboard Cite

Seed germination that begins with imbibition and ends with radicle emergence is the first step for plant growth. Successful germination is not only crucial for seedling establishment but also important for crop yield. After being dispersed from mother plant, seed undergoes continuous desiccation in ecosystem and selects proper environment to trigger germination. Owing to the contribution of transcriptomic, proteomic, and molecular biological studies, molecular aspect of seed germination is elucidated well in Arabidopsis. Recently, more and more proteomic and genetic studies concerning cereal seed germination were performed on rice (Oryza sativa) and barley (Hordeum vulgare), which possess completely different seed structure and domestication background with Arabidopsis. In this review, both the common features and the distinct mechanisms of seed germination are compared among different plant species including Arabidopsis, rice, and maize. These features include morphological changes, cell and its related structure recovery, metabolic activation, hormone behavior, and transcription and translation activation. This review will provide more comprehensive insights into the molecular mechanisms of seed germination.

show abstract

Identification of QTLs for germination and seedling development in sunflower (Helianthus annuus L.)

Cited by 23 publications

References 21 publications

Complex genetics controls natural variation among seed quality phenotypes in a recombinant inbred population of an interspecific cross between Solanum lycopersicum × Solanum pimpinellifolium

Complex genetics controls natural variation among seed quality phenotypes in a recombinant inbred population of an interspecific cross between Solanum lycopersicum × Solanum pimpinellifolium

Genetic mapping within the wheat D genome reveals QTL for germination, seed vigour and longevity, and early seedling growth

Studies on the molecular mechanisms of seed germination

Contact Info

Product

Resources

About