Identification of QTL associated with flower and runner production in octoploid strawberry (Fragaria×ananassa)

Abstract

BACKGROUND: Understanding the genetics of flowering in the strawberry (Fragaria × ananassa) will aid in the development of breeding strategies. OBJECTIVE: To search for quantitative trait loci (QTL) associated with remontancy and weeks of flowering in the strawberry. METHODS: Previously collected phenotypic data from two non-remontant 'Honeoye' × remontant 'Tribute' strawberry populations and simple sequence repeats (SSR) markers were used to search for QTL associated with repeat flowering, weeks of flowering and runner production, as well as the ability to produce flowers and runners at 17, 20 and 23 • C. RESULTS: As was discovered in other studies, we found a major QTL that regulated remontancy and weeks of flowering on homeologous linkage group IV of 'Tribute'. This QTL also had a negative effect on runner production and a positive influence on flower production under high temperatures. A number of additional QTL were discovered that significantly (LOD >3.0) influenced flower and runner production. CONCLUSIONS: Remontancy/non-remontancy is controlled by a major gene/locus and several minor modifying ones.

High density linkage map construction and QTL mapping for runner production in allo-octoploid strawberry Fragaria × ananassa based on ddRAD-seq derived SNPs

“…15 was further confirmed by Sooriyapathirana et al . 33 using an extended population. Using three separate Japanese population, Honjo et al .…”

“…Several studies have constructed linkage maps using different suite of molecular markers such as RAPD, AFLP and SSR, etc. 27–32 and few of those studies reported QTLs with various additive effects for PF and RU traits in cultivated strawberry 3,4,15,17,33–35 . However, these markers largely suffer from either non-transferability across investigations, insufficient genome-wide coverage, poor density or higher developmental and screening costs 3,15,36 .…”

Genotyping-by-sequencing enables linkage mapping in three octoploid cultivated strawberry families

“…The strawberry samples analyzed in this study consisted of: parents and 24 offspring from the ‘Holiday’ × ‘Korona’ population from the Netherlands ( Van Dijk et al, 2014 ); parents and 60 seedlings from the ‘Tribute’ × ‘Honeoye’ population from Michigan State University (MSU) ( Castro & Lewers, 2016 ; Sooriyapathirana et al, 2015 ); parents and 51 offspring from the ‘Redgauntlet’ × ‘Hapil’ population from East Malling Research, UK ( Sargent et al, 2012 ; Table S1 ). DNA was extracted from actively growing leaf tissue with the E-Z 96® Plant DNA extraction kit (Omega BioTek, Norcross, GA, USA) as previously described ( Gilmore, Bassil & Hummer, 2011 ).…”

Pedigree-Based Analysis in a Multiparental Population of Octoploid Strawberry Reveals QTL Alleles Conferring Resistance toPhytophthora cactorum

“…The development of molecular tools for cultivated strawberry has been hindered in the past by its allo-octoploid (2 n = 8 × = 56) genetic constitution, limiting the development of dense linkage maps and high-throughput marker resources ( Sargent et al 2009 ). For this reason, only a few studies have reported major genes and QTL in cultivated strawberry to date ( Van de Weg 1997a , b ; Haymes et al 1997 , 2000 ; Weebadde et al 2008 ; Zorrilla-Fontanesi et al 2011 , 2012 ; Lerceteau-Köhler et al 2012 ; Antanaviciute et al 2015 ; Sooriyapathirana et al 2015 ; Roach et al 2016 ). However, the sequencing of the diploid woodland strawberry ( F. vesca ) genome ( Shulaev et al 2011 ) set the stage for the development of the Affymetrix IStraw90 Axiom array, a 90 K SNP array for octoploid strawberry ( Bassil et al 2015 ).…”