Identification of pterostilbene as a phytoalexin from Vitis vinifera leaves

Langcake, P.; Cornford, C.A.; Pryce, Robert J.

doi:10.1016/s0031-9422(00)91470-5

Cited by 201 publications

(124 citation statements)

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“…To characterize a putative ROMT from grapevine, the variety Cabernet Sauvignon was chosen because it was shown previously to accumulate pterostilbene upon fungal infection (Langcake et al, 1979). Indeed, inoculation of Cabernet Sauvignon leaves with P. viticola resulted in the accumulation of various stilbenes, including trans-resveratrol, trans-and cis-piceid, viniferins, and pterostilbene (Fig.…”

Section: Biosynthesis Of Pterostilbene In Grapevine Leaves Infected Wmentioning

confidence: 99%

“…Pterostilbene has also been identified as a phytoalexin in grapevine (Vitis vinifera). Together with resveratrol and its derivatives piceid and viniferins, pterostilbene accumulates in grapevine leaves infected by Plasmopara viticola (Langcake et al, 1979), and low amounts of pterostilbene are also produced during the preparation of grapevine protoplasts (Commun et al, 2003). This compound was also found in healthy grape berries as a constitutive stilbene (Pezet and Pont, 1988).…”

mentioning

confidence: 99%

“…This compound was also found in healthy grape berries as a constitutive stilbene (Pezet and Pont, 1988). Like resveratrol, pterostilbene was shown to possess antifungal activity against various grapevine pathogens (Langcake et al, 1979). Indeed, pterostilbene was 5 to 10 times more effective than resveratrol in inhibiting the germination of conidia of Botrytis cinerea and sporangia of P. viticola (Jeandet et al, 2002).…”

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confidence: 99%

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A Stress-Inducible Resveratrol O-Methyltransferase Involved in the Biosynthesis of Pterostilbene in Grapevine

et al. 2008

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Stilbenes are considered the most important phytoalexin group in grapevine (Vitis vinifera) and they are known to contribute to the protection against various pathogens. The main stilbenes in grapevine are resveratrol and its derivatives and, among these, pterostilbene has recently attracted much attention due both to its antifungal and pharmacological properties. Indeed, pterostilbene is 5 to 10 times more fungitoxic than resveratrol in vitro and recent studies have shown that pterostilbene exhibits anticancer, hypolipidemic, and antidiabetic properties. A candidate gene approach was used to identify a grapevine resveratrol O-methyltransferase (ROMT) cDNA and the activity of the corresponding protein was characterized after expression in Escherichia coli. Transient coexpression of ROMT and grapevine stilbene synthase in tobacco (Nicotiana benthamiana) using the agroinfiltration technique resulted in the accumulation of pterostilbene in tobacco tissues. Taken together, these results showed that ROMT was able to catalyze the biosynthesis of pterostilbene from resveratrol both in vitro and in planta. ROMT gene expression in grapevine leaves was induced by different stresses, including downy mildew (Plasmopara viticola) infection, ultraviolet light, and AlCl 3 treatment.

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Section: Biosynthesis Of Pterostilbene In Grapevine Leaves Infected Wmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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A Stress-Inducible Resveratrol O-Methyltransferase Involved in the Biosynthesis of Pterostilbene in Grapevine

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“…Recently, supplementation of Resveratrol (3,4′, 5-trihydroxystilbene), a naturally occurring phytoalexin, has been found to have beneficial impact on porcine IVM and embryonic development [21]. Resveratrol is produced by the interaction of plants with a microorganism present in the roots of Polygonum cuspidatum , Vitis vinifera , red wines, and mulberries and protects these plants against fungal and bacterial infections [23]. Resveratrol exhibits wide variety of pharmacological properties like anti-inflammatory, chemopreventive, antioxidant, antiproliferative, proapoptotic, cardioprotective, and anticancer effects [12,33].…”

Section: Introductionmentioning

confidence: 99%

Resveratrol treatment during goat oocytes maturation enhances developmental competence of parthenogenetic and hand-made cloned blastocysts by modulating intracellular glutathione level and embryonic gene expression

Mukherjee

Malik

Saha

et al. 2013

J Assist Reprod Genet

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Purpose The aim of the present study was to determine whether supplementation of resveratrol, a stilbenoid antioxidant with therapeutic significance, influences goat (Capra hircus) oocyte maturation and subsequent embryonic development and expression of apoptosis and early embryonic development-related genes. Methods Five different concentrations of resveratrol (0.1, 0.25, 0.5, 2.0 and 5.0 μM) were used in in vitro maturation (IVM) medium. Cell tracker blue and 2′,7′-dichlorodihydrofluorescein diacetate (H 2 DCFDA) fluorescent stains were used to assay intracellular glutathione and reactive oxygen species levels in mature oocytes. Parthenogenetic activation and hand-made cloning were performed to check the developmental potential following resveratrol treatment. We used quantitative real-time PCR to analyze embryonic gene expression. Result Compared to control, no significant improvement was observed in nuclear maturation in resveratrol-treated groups and at 5.0 μM concentration maturation rate decreased significantly (P <0.05). But resveratrol treatment at the concentrations of 0.25, 0.5 μM significantly reduced intracellular ROS, and increased GSH concentrations. Oocytes treated with 0.25, 0.5 μM resveratrol when subsequently used for PA and HMC, higher extent of blastocyst yields were observed. Expression analysis of proapoptotic (Bax) gene in mature oocytes, cumulus cells, and HMC-derived blastocysts revealed lesser transcript abundances in various resveratrol-treated groups., however no change in the same was observed for antiapoptotic gene (Bcl2). Differential expression of genes associated with developmental competence and nuclear reprogramming was also observed in HMC-derived blastocysts. Conclusion Our results show that resveratrol treatment at optimum concentrations (0.25 and 0.5 μM) during IVM produced beneficial microenvironment within oocytes by increasing the intracellular GSH, decreasing ROS level and this in turn, stimulated embryonic development and regulated gene expression.

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“…Some flavonoids, 12 diterpenoids, 13,14 sequiterpenoids, 15 monoterpenoids, 16 stilbenes, 17,18 steroidal glycoalkaloid [19][20][21] and triterpenoids 22 have effect against this fungus (inhibition mycelia growth, cytological changes, toxic action and protection cucumber tissue, etc).…”

Section: Introductionmentioning

confidence: 99%

In Vitro Sensitivity of Botrytis Cinérea to Resinous Exudates of Heliotropium Filifoliumand Geranyl Derivatives Compounds

Mendoza

Modak

Torres

et al. 2008

J. Chil. Chem. Soc.

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The activity of the extract of Heliotropium filifolium, of the natural compounds obtained from its resinous exudates, filifolinol and filifolinyl senecionate, and of the synthetic derivative of filifolinol, filifolinoic acid, on the mycelial growth of the phytopathogenic fungus Botrytis cinerea was analyzed in liquid and solid media. The extract of H. filifolium, filifolinol, and filifolinoic acid reduced mycelial growth of this fungus. The effect of these compounds was dose-dependant. The compound filifolinyl senecionate was inactive in all the tested conditions. The extract of H. filifolium and filifolinol retarded the germination of conidia of the fungus.

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Identification of pterostilbene as a phytoalexin from Vitis vinifera leaves

Cited by 201 publications

References 10 publications

A Stress-Inducible Resveratrol O-Methyltransferase Involved in the Biosynthesis of Pterostilbene in Grapevine

A Stress-Inducible Resveratrol O-Methyltransferase Involved in the Biosynthesis of Pterostilbene in Grapevine

Resveratrol treatment during goat oocytes maturation enhances developmental competence of parthenogenetic and hand-made cloned blastocysts by modulating intracellular glutathione level and embryonic gene expression

In Vitro Sensitivity of Botrytis Cinérea to Resinous Exudates of Heliotropium Filifoliumand Geranyl Derivatives Compounds

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