Identification of prototype compounds and their metabolites in rats’ serum from Xuefu Zhuyu Decoction by UPLC-Q-TOF/MS

Zhang, Xiaoyu; Jiang, Zhenzuo; Zhang, Lei; Cheng, Xue; Feng, Xiafei; Chai, Xin; Wang, Yuefei

doi:10.1016/j.chmed.2022.08.002

Cited by 4 publications

(3 citation statements)

References 32 publications

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“…Then, we analyzed the compounds by pattern recognition in EZinfo2.0. [67] To further analyze and compare the differences in metabolites among different groups, principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were applied to analyze the metabolic profile data of the control group and CUMS group in the positive ion modes (POS) and negative ion modes (NEG). Among the ions in the obtained S-Plot diagram, the potential depression markers meeting variable importance in the projection (VIP) > 1 and P < 0.05 were screened as candidates.…”

Section: Metabolic Profilingmentioning

confidence: 99%

Two Omics Methods Expose Anti‐Depression Mechanism of Raw and Vinegar‐Baked Bupleurum Scorzonerifolium Willd

Qu,

Zheng,

et al. 2024

Chemistry & Biodiversity

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Bupleurum scorzonerifolium willd. (BS) and its vinegar‐baked product (VBS) has been frequently utilized for depression management in clinical Chinese medicine. This paper aims to elucidate the antidepressant mechanism of BS and VBS from the perspectives of metabonomics and gut microbiota. A rat model of depression was established by CUMS combined with feeding alone to evaluate the antidepressant effects of BS and VBS. UPLC‐Q‐TOF‐MS/MS‐based metabolomics and 16S rRNA sequencing of rat feces were applied and the correlation of differential metabolic markers and intestinal floras was analyzed. The result revealed that BS and VBS significantly improved depression‐like behaviors and the levels of monoamine neurotransmitters in CUMS rats. There were 27 differential endogenous metabolites between CUMS and normal rats, which were involved in 8 metabolic pathways. Whereas, BS and VBS could regulate 18 and 20 metabolites respectively, wherein fifteen of them were shared metabolites. On the genus level, BS and VBS could regulate twenty‐five kinds of intestinal floras in CUMS rats, that is, they increased the abundance of beneficial bacteria and decreased the abundance of harmful bacteria. In conclusion, both BS and VBS exert excellent antidepressant effects by regulating various metabolic pathways and ameliorating intestinal microflora dysfunction.

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Section: Metabolic Profilingmentioning

confidence: 99%

Two Omics Methods Expose Anti‐Depression Mechanism of Raw and Vinegar‐Baked Bupleurum Scorzonerifolium Willd

Qu,

Zheng,

et al. 2024

Chemistry & Biodiversity

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“…JZOL prescription was derived from the experience of TCM in preventing and treating acute respiratory infections and is suitable for the "phlegm heat syndrome" [14][15][16] . Using UPLC-Q/TOF-MS technology analysis, the main components absorbed into the bloodstream included flavonoids, triterpenes, and anthraquinones; the main active ingredients were flavonoids represented by baicalin 17 .…”

Section: Introductionmentioning

confidence: 99%

Depicting the regulatory role of JZOL on TRP channels in the treatment of Acute Bronchitis based on the combination of clinical trials, computational analysis and in vivo experiments

Fan,

Wu,

et al. 2024

Preprint

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The comparison between traditional Chinese medicine Jinzhen Oral Liquid (JZOL) and western medicine in treating children with acute bronchitis (AB) showed encouraging outcomes. This trial evaluated the efficacy and safety of the JZOL for improving cough and expectoration in children with AB. 480 children were randomly assigned to take JZOL or Ambroxol Hydrochloride and Clenbuterol Hydrochloride Oral Solution for 7 days. The primary outcome was time-to-cough resolution. The median time-to-cough resolution in both groups was 5.0 days and the antitussive onset median time was only 1 day. This head to head randomized controlled trial showed that JZOL was not inferior to cough suppressant and phlegm resolving western medicine in treating cough and sputum and could comprehensively treat respiratory and systemic discomfort symptoms. Combined with clinical trials, the mechanism of JZOL against AB was uncovered by network target analysis, it was found that the pathways in TRP channels like IL-1β;/IL1R/TRPV1/TRPA1, NGF/TrkA/TRPV1/TRPA1 and PGE2/EP/PKA/TRPV1/TRPA1 might play important roles. Animal experiments further confirmed that inflammation and immune regulatory effect of JZOL in the treatment of AB were of vital importance and TRP channels was the key mechanism of action.

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“…Ultra-performance liquid chromatography-tandem quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS E ) is a useful method for this purpose due to its high sensitivity, selectivity, and ability to provide complete and high-quality mass spectrometry data. 4,5 This method is simple and efficient 6,7 and has been successfully applied in many fields, 8 including Chinese herbal medicine, 9–12 natural product research and development, 13,14 microorganisms, 15 and the food industry. It serves as a highly effective tool for elucidating the chemical composition profile of substances.…”

Section: Introductionmentioning

confidence: 99%

UPLC-Q-TOF-MS^E Based Profiling of the Chemical Composition of Different Parts of Tetrastigma hemsleyanum

Peng,

Yin,

et al. 2024

Natural Product Communications

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Objectives: The differences in chemical constituents in different parts of Tetrastigma hemsleyanum were analyzed. Methods: The contents of total phenols, total flavonoids, and polysaccharides in methanol extracts from different parts of T. hemsleyanum in Zhejiang were determined, and the chemical components of different parts of T. hemsleyanum were collected by ultra-performance liquid chromatography-tandem quadrupole-time-of-flight mass spectrometry. The differential index components of different parts of T. hemsleyanum were identified and analyzed by accurate mass-to-charge ratio of primary mass spectrometry and fragment ion of secondary mass spectrometry with reference spectrum and software database search and related literature reports. Results: A total of 20 components were identified, including 13 identified in root tubers, 7 in fibrous roots and 8 in leaves, and the structural cleavage of its compounds was analyzed. Conclusion: It provides a possibility and theoretical basis for the future use of the whole plant of T. hemsleyanum as medicine.

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Identification of prototype compounds and their metabolites in rats’ serum from Xuefu Zhuyu Decoction by UPLC-Q-TOF/MS

Cited by 4 publications

References 32 publications

Two Omics Methods Expose Anti‐Depression Mechanism of Raw and Vinegar‐Baked Bupleurum Scorzonerifolium Willd

Two Omics Methods Expose Anti‐Depression Mechanism of Raw and Vinegar‐Baked Bupleurum Scorzonerifolium Willd

Depicting the regulatory role of JZOL on TRP channels in the treatment of Acute Bronchitis based on the combination of clinical trials, computational analysis and in vivo experiments

UPLC-Q-TOF-MS^E Based Profiling of the Chemical Composition of Different Parts of Tetrastigma hemsleyanum

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Identification of prototype compounds and their metabolites in rats’ serum from Xuefu Zhuyu Decoction by UPLC-Q-TOF/MS

Cited by 4 publications

References 32 publications

Two Omics Methods Expose Anti‐Depression Mechanism of Raw and Vinegar‐Baked Bupleurum Scorzonerifolium Willd

Two Omics Methods Expose Anti‐Depression Mechanism of Raw and Vinegar‐Baked Bupleurum Scorzonerifolium Willd

Depicting the regulatory role of JZOL on TRP channels in the treatment of Acute Bronchitis based on the combination of clinical trials, computational analysis and in vivo experiments

UPLC-Q-TOF-MSE Based Profiling of the Chemical Composition of Different Parts of Tetrastigma hemsleyanum

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UPLC-Q-TOF-MS^E Based Profiling of the Chemical Composition of Different Parts of Tetrastigma hemsleyanum