Identification of proteome, antigen protein and antigen membrane protein from<i>Spiroplasma eriocheiris</i>

Liu, Yuhan; Xu, Yi; Li, Shuang; Xu, Xuechuan; Gao, Qi; Yuan, Meijun; Gu, Wei; Wang, Wen; Meng, Qingguo

doi:10.1111/lam.12784

Cited by 6 publications

(8 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, heat shock protein 70, pyruvate dehydrogenase E1‐beta subunit, elongation factor Tu and the P76 membrane protein of Mycoplasma hyopneumoniae were determined as antigenic protein (Pinto et al, ). The SPE0313 protein is proved as membrane antigenic protein of S. eriocheiris by proteomics analysis (Liu et al, ), but its characteristics and functions were not studied clearly.…”

Section: Resultsmentioning

confidence: 99%

“…Except adhesion‐like protein ALP, spiralin‐like protein SLP25 and SLP31 were immunoproteins (Meng, Gu, Bi, Ji, & Wang, ; Meng, Ou et al, ), lysophospholipase protein from S. eriocheiris lied in membrane and cytoplasm, the virulence ability was decreased obviously through antibody neutralization test (Zhu et al, ). SPE0313 is an antigenic membrane proteins of S. eriocheiris (Liu et al, ), but the function of SPE0313 in adhesion and invasion of E. sinensis were not clear. Since UGA is not a termination signal but reads as a tryptophan codon in mollicute (Citti, Maréchaldrouard, Saillard, Wei, & Bové, ; Stamburski, Renaudin, & Bové, ), SPE0313 has been site‐muted to express the whole protein in Escherichia coli after gene clone.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

SPE0313 located at cell membrane of Spiroplasma eriocheiris is required for adhesion and invasion Eriocheir sinensis hemocytes

Hao

Gao

Jian

et al. 2019

Journal of Fish Diseases

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

SPE0313 located at cell membrane of Spiroplasma eriocheiris is required for adhesion and invasion Eriocheir sinensis hemocytes

Hao

Gao

Jian

et al. 2019

Journal of Fish Diseases

View full text Add to dashboard Cite

“…PK can catalyze phosphate group transfer and phosphoenolpyruvate conversion to adenosine diphosphate [51, 52]. PK not only takes part in carbohydrate metabolism [53–55] but is also involved in immunological reaction progress in crustaceans [56, 57]. A study on Drosophila suzukii found that photoperiod could regulate the PK levels, and then mediate the adult reproductive diapause [58].…”

Section: Discussionmentioning

confidence: 99%

The transcriptome sequencing and functional analysis of eyestalk ganglions in Chinese mitten crab (Eriocheir sinensis) treated with different photoperiods

Pang

Zhang

et al. 2019

PLoS ONE

View full text Add to dashboard Cite

Photoperiod plays an important role in individual growth, development, and metabolism in crustaceans. The growth and reproduction of crabs are closely related to the photoperiod. However, as of yet, there are still no transcriptomic reports of eyestalk ganglions treated under different photoperiods in the Chinese mitten crab (Eriocheir sinensis), which is a benthonic crab with high commercial value in Asia. In this study, we collected the eyestalk ganglions of crabs that were reared under different photoperiods, including a control group (L: D = 12 h: 12 h, named CC), a constant light group (L: D = 24 h: 0 h, named LL) and a constant darkness group (L: D = 0 h: 24 h, named DD). RNA sequencing was performed on these tissues in order to examine the effects of different photoperiods. The total numbers of clean reads from the CC, LL and DD groups were 48,772,584 bp, 53,943,281 bp and 53,815,178 bp, respectively. After de novo assembly, 161,380 unigenes were obtained and were matched with different databases. The DEGs were significantly enriched in phototransduction and energy metabolism pathways. Results from RT-qPCR showed that TRP channel protein (TRP) in the phototransduction pathway had a significantly higher level of expression in LL and DD groups than in the CC group. We found that the downregulation of the pyruvate dehydrogenase complex (PDC) gene and the upregulation phosphoenolpyruvate carboxykinase (PPC) gene were involved in energy metabolism processes in LL or DD. In addition, we also found that the upregulation of the expression level of the genes Gαq, pyruvate kinase (PK), NADH peroxidase (NADH) and ATPase is involved in phototransduction and energy metabolism. These results may shed some light on the molecular mechanism underlying the effect of photoperiod in physiological activity of E. sinensis.

show abstract

“…After washing and resuspension, the sample was sonicated on ice and centrifuged to remove the cellular debris. Rabbit antiserum against S. mirum was prepared by following the methods described by Liu Y. et al (2017) . The serum was conjugated to Protein A Beads at 4°C for 4 h with gentle shaking.…”

Section: Methodsmentioning

confidence: 99%

“…Immunoproteomics as an emerging field of proteome analysis plays a key role in screening immunogenic proteins. Such a method has been used to successfully identify antigenic epitopes in several pathogens, such as Mycoplasma bovis , Brucella abortus , Neisseria meningitidis , and S. eriocheiris ( Liu Y. et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

The genome and antigen proteome analysis of Spiroplasma mirum

Liu

Li²,

Ye³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Spiroplasma mirum, small motile wall-less bacteria, was originally isolated from a rabbit tick and had the ability to infect newborn mice and caused cataracts. In this study, the whole genome and antigen proteins of S. mirum were comparative analyzed and investigated. Glycolysis, pentose phosphate pathway, arginine metabolism, nucleotide biosynthesis, and citrate fermentation were found in S. mirum, while trichloroacetic acid, fatty acids metabolism, phospholipid biosynthesis, terpenoid biosynthesis, lactose-specific PTS, and cofactors synthesis were completely absent. The Sec systems of S. mirum consist of SecA, SecE, SecDF, SecG, SecY, and YidC. Signal peptidase II was identified in S. mirum, but no signal peptidase I. The relative gene order in S. mirum is largely conserved. Genome analysis of available species in Mollicutes revealed that they shared only 84 proteins. S. mirum genome has 381 pseudogenes, accounting for 31.6% of total protein-coding genes. This is the evidence that spiroplasma genome is under an ongoing genome reduction. Immunoproteomics, a new scientific technique combining proteomics and immunological analytical methods, provided the direction of our research on S. mirum. We identified 49 proteins and 11 proteins (9 proteins in common) in S. mirum by anti-S. mirum serum and negative serum, respectively. Forty proteins in S. mirum were identified in relation to the virulence. All these proteins may play key roles in the pathogeny and can be used in the future for diagnoses and prevention.

show abstract

Identification of proteome, antigen protein and antigen membrane protein fromSpiroplasma eriocheiris

Cited by 6 publications

References 33 publications

SPE0313 located at cell membrane of Spiroplasma eriocheiris is required for adhesion and invasion Eriocheir sinensis hemocytes

SPE0313 located at cell membrane of Spiroplasma eriocheiris is required for adhesion and invasion Eriocheir sinensis hemocytes

The transcriptome sequencing and functional analysis of eyestalk ganglions in Chinese mitten crab (Eriocheir sinensis) treated with different photoperiods

The genome and antigen proteome analysis of Spiroplasma mirum

Contact Info

Product

Resources

About