Identification of Proteins Using Four Methods of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

Bendinskas, Kestutis; Canning, Ashley J.; Smith, A

doi:10.1021/acs.jchemed.8b00687

Cited by 4 publications

(4 citation statements)

References 12 publications

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“…The three-week experiment described by Bendinskas et al has introduced the students to the application of MALDI method in proteomic. 91 Four individual proteins (bovine serum albumin, equine myoglobin, bovine calmodulin and bovine cytochrome c) were identified by using four MALDI methods, linear positive, in-source decay, peptide mass fingerprint and post source decay.…”

Section: Proteomic Mass Spectrometrymentioning

confidence: 99%

Teaching mass spectrometry: A compilation of approaches to teaching theory and practice of mass spectrometry

Frański

2024

Eur J Mass Spectrom (Chichester)

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The areas of mass spectrometry applications seem to be much larger than those of any other analytical techniques. They extend from the determination of molecular mass in organic chemistry, through the analytical applications in forensic, environmental and omics sciences, the application in extra-terrestrial exploration and many others. Mass spectrometry, usually coupled with chromatographic techniques, has also found wide application in the pharmaceutical industry, forensic laboratories, laboratories of sanitary inspection or environmental inspection, etc. The growing areas of applications give rise to the demand for the comprehensive mass spectrometry education of undergraduates. This overview covers the body of literature describing various interesting ideas that can be successfully used for teaching mass spectrometry. Since mass spectrometry is a multidisciplinary field, old but dynamically developing, teaching mass spectrometry may be more problematic in comparison to teaching other analytical techniques, for example, there is the problem of position of mass spectrometry in the chemistry curriculum. On the other hand, it is obvious that the mass spectrometry community, besides difficult scientific work, does great and admirable teaching work, in order to perfectly educate undergraduates in the field of mass spectrometry and to make learning mass spectrometry as attractive as possible.

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Section: Proteomic Mass Spectrometrymentioning

confidence: 99%

Teaching mass spectrometry: A compilation of approaches to teaching theory and practice of mass spectrometry

Frański

2024

Eur J Mass Spectrom (Chichester)

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“…There are also several instrumental methods, ranging from gel electrophoresis , to mass spectrometry (MS) methods, ,− which have been used for protein identification and quantification. MS methods are selective and can detect food adulterations with nitrogen-rich compounds .…”

Section: Introductionmentioning

confidence: 99%

Whey Protein Analysis Using the Lowry Assay and 96-Well-Plate Digital Images Acquired Using Smartphones

Assink Junior,

de Jesus,

Borges

2023

J. Chem. Educ.

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Here, students determine the total protein content in whey protein samples using the Lowry assay. Quantitative analysis was carried out using absorbance measured at 750 nm (standard method) and 96-well-plate digital images obtained using smartphones (proposed method). The proposed method was carried out using two smartphones (1 and 2). Smartphone 1 had a higher resolution than smartphone 2. The statistical evaluation of the data set produced by the students was carried out using JASP (Jeffreys's Amazing Statistics Program), and it was a valuable tool to teach descriptive statistics and hypothesis tests. We chose JASP because it is free and open-source software with a graphical user interface. The protein percentage found in five whey protein samples using the standard method during three lab classes was compared using one-way and two-way ANOVA. Five whey protein samples were analyzed using the standard method and the proposed methods (smartphones 1 and 2). The results were compared using one-way repeated measure ANOVA (RMANOVA) and mixed-factor ANOVA. During the statistical analysis of the data set, students identified outlier results using boxplots and checked data normality using the Shapiro−Wilk test, histograms, and boxplots. They learned the difference between dependent and independent variables and compared variances using Levene's test (dependent variables) and Mauchly's test (independent variables). The interpretation of statistical tests was also carried out using descriptives plots, boxplots, and raincloud plots.

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“…The importance of mass spectrometry for proteomics makes it a relevant topic to be taught to undergraduate students. A number of initiatives with that purpose are found in the literature, some of which are very comprehensive, including hands-on activities from protein separation by electrophoresis to identification to mass spectrometry. − However, many of the proposed classes or courses focus on mass spectrometry itself, giving little or no attention to the principles of database searching. Although database searching is usually mentioned, instructors treat it as a self-explanatory procedure that deserves little attention.…”

Section: Introductionmentioning

confidence: 99%

Protein Identification by Database Searching of Mass Spectrometry Data in the Teaching of Proteomics

2021

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Mass spectrometry is essential for large-scale protein identification in proteomics. By database searching of mass spectrometry data, it is possible to identify proteins without the need to interpret MS/MS spectra (de novo sequencing), which is time-consuming and not feasible as a systematic tool in proteomic analysis. Many authors have reported initiatives to teach protein mass spectrometry to undergraduate students, but database searching is usually not emphasized. Here, we propose a class about database searching of mass spectrometry data for protein identification in order to complement a theoretical proteomics course. It includes a presentation about the theory of database searching and a practical, computer-based activity in which students can learn how to perform analysis of mass spectrometry data for proteomics purposes. Our class model fills a widespread gap in the teaching of proteomics and improves students’ understanding of protein identification by mass spectrometry.

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Identification of Proteins Using Four Methods of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

Cited by 4 publications

References 12 publications

Teaching mass spectrometry: A compilation of approaches to teaching theory and practice of mass spectrometry

Teaching mass spectrometry: A compilation of approaches to teaching theory and practice of mass spectrometry

Whey Protein Analysis Using the Lowry Assay and 96-Well-Plate Digital Images Acquired Using Smartphones

Protein Identification by Database Searching of Mass Spectrometry Data in the Teaching of Proteomics

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