Identification of proteins in susceptible and resistant Brassica oleracea responsive to Xanthomonas campestris pv. campestris infection

Villeth, Gabriela R. C.; Carmo, Lílian S.T.; Silva, Luciano; Santos, M. F.; Neto, Osmundo Brilhante de Oliveira; Grossi-de-Sá, Maria Fátima; Ribeiro, Igor Sousa; Dessaune, S. N.; Fragoso, Rodrigo da Rocha; Franco, Octávio L.; Mehta, Ângela

doi:10.1016/j.jprot.2016.01.014

Cited by 22 publications

(12 citation statements)

References 37 publications

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“…This reduction in photosynthetic activity supports the hypothesis of resources reallocation toward defense or signaling pathways. Related results were obtained by Villeth et al ., where photosynthetic proteins were decreased in infected plants. Moreover, those authors found that resistance was correlated with the ability of the plant to keep sufficient photosynthetic activity, and therefore had the energy necessary to trigger defense mechanisms.…”

Section: Discussionsupporting

confidence: 80%

Unraveling the metabolic response of Brassica oleracea exposed to Xanthomonas campestris pv. campestris

et al. 2018

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Section: Discussionsupporting

confidence: 80%

Unraveling the metabolic response of Brassica oleracea exposed to Xanthomonas campestris pv. campestris

et al. 2018

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“…Malate dehydrogenase, an important malate metabolizing enzyme, has been associated with plant defense, suggesting that the increased abundance of this enzyme can provide resources for biosynthesis of defense compounds (Rhodes et al, 1968; Walter et al, 1988; Casati et al, 1999). In a previous study, one MDH showed increased abundance in brassica–Xcc resistant interaction and was associated with the activation of photosynthetic metabolism (Villeth et al, 2016). Other metabolism proteins such as fructose-1,6-biphosphate, cytosolic EC 3.1.3.11 (A0A0D3BSL1), basic endochitinase CHI-B4-like, EC 3.2.1.14 (A0A0D3B6J8; A0A0D3BPL2) and UDP-arabinopyranose mutase 1-like (A0A0D3B9D8), were increased in RI (9-, 12-, 9- and 6-fold, respectively) and the first two (A0A0D3BSL1 and A0A0D3B6J8) were decreased in SI (28- and 12-fold, respectively), while UDP-arabinopyranose level was unchanged in SI.…”

Section: Resultsmentioning

confidence: 93%

“…In this work, we also analyzed the gene expression levels by qRT-PCR of 30 selected genes encoding the differential proteins identified (Figure 2 and Table 1), based on biological process (defense-related), fold-change (≥1.5 increased or decreased in both cultivars) and previous studies (Villeth et al, 2016; Ribeiro et al, 2018). As expected, the expression levels of many mRNAs did not correlate with protein abundance and different clusters could be observed in the heatmap generated to compare these levels (Figure 2).…”

Section: Resultsmentioning

confidence: 99%

Proteomic Analysis and Functional Validation of a Brassica oleracea Endochitinase Involved in Resistance to Xanthomonas campestris

et al. 2019

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Black rot is a severe disease caused by the bacterium Xanthomonas campestris pv. campestris (Xcc), which can lead to substantial losses in cruciferous vegetable production worldwide. Although the use of resistant cultivars is the main strategy to control this disease, there are limited sources of resistance. In this study, we used the LC-MS/MS technique to analyze young cabbage leaves and chloroplast-enriched samples at 24 h after infection by Xcc, using both susceptible (Veloce) and resistant (Astrus) cultivars. A comparison between susceptible Xcc-inoculated plants and the control condition, as well as between resistant Xcc-inoculated plants with the control was performed and more than 300 differentially abundant proteins were identified in each comparison. The chloroplast enriched samples contributed with the identification of 600 additional protein species in the resistant interaction and 900 in the susceptible one, which were not detected in total leaf sample. We further determined the expression levels for 30 genes encoding the identified differential proteins by qRT-PCR. CHI-B4 like gene, encoding an endochitinase showing a high increased abundance in resistant Xcc-inoculated leaves, was selected for functional validation by overexpression in Arabidopsis thaliana. Compared to the wild type (Col-0), transgenic plants were highly resistant to Xcc indicating that CHI-B4 like gene could be an interesting candidate to be used in genetic breeding programs aiming at black rot resistance.

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“…However, resistance to Xcc in B . oleracea was previously related to the ability of the plant to keep sufficient photosynthesis metabolism activity to provide energy supplies necessary for defence 32–34 .…”

Section: Discussionmentioning

confidence: 99%

Dissecting quantitative resistance to Xanthomonas campestris pv. campestris in leaves of Brassica oleracea by QTL analysis

Iglesias-Bernabé

Madloo

Rodríguez

et al. 2019

Sci Rep

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Black rot, caused by the bacterium Xanthomonas campestris pv. campestris (Xcc), produces important economic losses in crops of Brassica oleracea worldwide. Resistance to race 1, the most virulent and widespread in B. oleracea, is under quantitative control. Knowledge about the genetics of this resistance would help in designing strategies to control initial stages of invasion and development of the disease. QTL analysis of the resistance in the BolTBDH mapping population was performed. Resistance was measured with five traits related to initial stages of the invasion, success of infection and spread of the pathogen. Four single-trait QTLs of resistance were found, from which one represent novel variation. After performing multi-trait QTL, we concluded that spread of Xcc is related to the size of the leaf. Individuals from the mapping population follow two different strategies to cope with the spread of the disease: reducing lesion size or maintain more area of the leaf photosynthetically active, being more tolerant to Xcc invasion. Mechanisms underlying variation for resistance may be related to different aspects of plant immunity, including the synthesis of glucosinolates and phenolics.

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Identification of proteins in susceptible and resistant Brassica oleracea responsive to Xanthomonas campestris pv. campestris infection

Cited by 22 publications

References 37 publications

Unraveling the metabolic response of Brassica oleracea exposed to Xanthomonas campestris pv. campestris

Unraveling the metabolic response of Brassica oleracea exposed to Xanthomonas campestris pv. campestris

Proteomic Analysis and Functional Validation of a Brassica oleracea Endochitinase Involved in Resistance to Xanthomonas campestris

Dissecting quantitative resistance to Xanthomonas campestris pv. campestris in leaves of Brassica oleracea by QTL analysis

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