Identification of Proteins in Human Cytomegalovirus (HCMV) Particles: the HCMV Proteome

Host-viral effects of chromatin assembly factor 1 interaction with HCMV IE2

“…Although HCMV virion contains no histone proteins [55,56], a number of reports indicate that host histones with different modifications associate with HCMV DNA in infected cells cultured in labs or isolated from humans [49,[57][58][59][60][61][62]. Nitzsche et al [43] further indicate that HCMV DNA is chromatinized in infected cells; histones are localized with UL44 at replication foci and there is a robust increase of histone occupancy coupled with viral DNA replication.…”

Exogenous introduction of an immunodominant peptide from the non-structural IE1 protein of human cytomegalovirus into the MHC class I presentation pathway by recombinant dense bodies

“…One major attribute of DB, i.e. the abundance of the major T-cell antigen pp65 (Varnum et al, 2004), renders these particles attractive for the development of a vaccine. However, recent studies showed that non-structural antigens, such as IE proteins, may also contribute significantly to the priming of a natural T-cell response against HCMV in humans ( Elkington et al, 2003;Khan et al, 2005;Manley et al, 2004;Sylwester et al, 2005).…”

“…They are released from infected fibroblast cultures and enter cells presumably via the normal HCMV entry processes. The major constituent of DB is pp65 (ppUL83) (Varnum et al, 2004), which is a prominent target of the CD4 and CD8 T-cell (CTL) responses following natural infection (Beninga et al, 1995;McLaughlin-Taylor et al, 1994;Wills et al, 1996). Although pp65 by itself appears to be an attractive antigen for the design of a vaccine, other HCMV proteins may also be important and should thus be considered (Reddehase, 2002).…”

Emerging functions as host cell factors – an encyclopedia of annexin-pathogen interactions

“…AnxA5 is associated with herpes simplex virus (HSV) type 1 (Loret et al, 2008), HIV-1 (Chertova et al, 2006), VSV (Moerdyk-Schauwecker et al, 2009, Rift Valley fever virus (Nuss et al, 2014), and IAV (Shaw et al, 2008). How and at which stage in the virus infection cycle annexins are acquired remains so far unclear (which Gershom et al, 2012Wright et al, 1994Wright et al, 1995;Pietropaolo and Compton 1997;Pietropaolo and Compton 1999;Raynor et al, 1999;Varnum et al, 2004;Derry et al, 2007 generally holds true for virus-embedded host cell proteins), and the association might simply reflect the nature of the specialized membrane domains where viral assembly and budding take place. Viruses that bud from lipidenriched plasma membrane domains ('rafts') would then incorporate the often raft-associated annexins as part of their host cell membrane-derived viral envelope.…”

“…Annexin A1 (AnxA1) is one of the most famous family members and there is a wealth of information on its protective anti-inflammatory and proresolving effects (Perretti and D'Acquisto, 2009 (Kattenhorn et al, 2004;Dry et al, 2008;Loret et al, 2008;Vidick et al, 2013), human immunodeficiency virus type 1 (HIV-1) (Chertova et al, 2006), vesicular stomatitis virus (VSV) (Moerdyk-Schauwecker et al, 2009, Rift Valley fever virus (Nuss et al, 2014) and IAV (Shaw et al, 2008), and recent evidence points to a function of the AnxA1 receptor FPR2 in IAV replication (Tcherniuk et al, 2016). AnxA2 is a component of herpesviruses (Wright et al, 1994(Wright et al, , 1995Varnum et al, 2004;Zhu et al, 2005;Dry et al, 2008;Loret et al, 2008;Gershom et al, 2012;Vidick et al, 2013), IAV (LeBouder et al, 2008;Shaw et al, 2008;Liu et al, 2012), HIV-1 (Chertova et al, 2006), human papilloma virus (HPV) type 16 (Woodham et al, 2012), hepatitis C virus (HCV) type 1 (Backes et al, 2010), VSV (Moerdyk-Schauwecker et al, 2009, vaccinia virus (Chung et al, 2006), Rift Valley fever virus (Nuss et al, 2014) and Newcastle disease virus (Ren et al, 2012). AnxA5 is associated with herpes simplex virus (HSV) type 1 (Loret et al, 2008), HIV-1 (Chertova et al, 2006), VSV (Moerdyk-Schauwecker et al, 2009, Rift Valley fever virus (Nuss et al, 2014), and IAV (Shaw et al, 2008).…”