Identification of Protein Scaffolds for Enzyme Design Using Scaffold Selection

Abstract

The identification of suitable protein structures that can serve as scaffolds for the introduction of catalytic residues is crucial for the design of new enzymes. Here we describe how the automated and rapid scaffold search program ScaffoldSelection can be used to find the best starting points, namely protein structures that are most likely to tolerate the introduction and promote the proper formation of a specific catalytic motif.

Combined in vitro and cell‐based selection display method producing specific binders against IL‐9 receptor in high yields

“…Protein scaffolds are used as alternatives and/or supplements to monoclonal antibodies for research [2], diagnostics [3] and treatment purposes [4] because they possess several advantages over the more frequently used monoclonal antibodies: they are smaller, nonglycosylated and easier to produce in prokaryotes. Of increasing importance seems to be that there is no need for animal immunization to produce them and that their production can be cheaper than the production of antibodies.…”