Identification of protein phosphatase interacting proteins from normal and UVA-irradiated HaCaT cell lysates by surface plasmon resonance based binding technique using biotin–microcystin-LR as phosphatase capturing molecule

Bécsi, Bálint; Dedinszki, Dóra; Gyémánt, Gyöngyi; Máthé, Csaba; Vasas, Gábor; Lontay, Beáta; Erdõdi, Ferenc

doi:10.1016/j.jphotobiol.2014.06.004

Cited by 4 publications

(2 citation statements)

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“…Previous reports revealed that PP2Ac interacts with the regulatory subunit (MYPT1) of myosin phosphatase in cells, which regulates motility of invasive stages of the parasite (Becsi et al. ; Green et al. ).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of serine/threonine protein phosphatase of Eimeria tenella

Zhao

Zhu

et al. 2020

J Eukaryotic Microbiology

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Avian coccidiosis is a widespread and economically significant poultry disease caused by several Eimeria species, including Eimeria tenella. Previously, E. tenella serine/threonine protein phosphatase (EtSTP) was found to be differentially expressed in drug‐sensitive (DS) and drug‐resistant strains using RNA‐seq. In the present study, we found that transcription and translation levels of EtSTP were higher in diclazuril‐resistant (DZR) strains and maduramicin‐resistant (MRR) strains than in DS strains using quantitative real‐time PCR (qPCR) and Western blotting. Enzyme activity results indicated that the catalytic activity of EtSTP was higher in the two drug‐resistant strains than in DS strains. Western blot and qPCR analysis also showed that expression levels of EtSTP were higher in unsporulated oocysts (UO) and second‐generation merozoites (SM). Indirect immunofluorescence localization showed that EtSTP was located in most areas of the parasite with the exception of refractile bodies, and fluorescence intensity was enhanced during development. In vitro inhibition experiments showed that the ability of sporozoites (SZ) to invade cells was significantly decreased after treatment with anti‐rEtSTP antibody. These results indicated that EtSTP acted mainly during the developmental and reproductive stages of the parasite and may be related to the resistance of coccidia to external drug pressure.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization of serine/threonine protein phosphatase of Eimeria tenella

Zhao

Zhu

et al. 2020

J Eukaryotic Microbiology

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show abstract

“…Effectiveness of the experiment, however, always depends on the optimal binding, washing and elution conditions, and resulting specificity and compatibility for the PPIs of interest. PPIs can be also quantified using surface plasmon resonance (SPR) (12,13), which has been used almost exclusively in validation experiments with purified proteins up-to-date (14)(15)(16)(17)(18), with a single exception (19).…”

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confidence: 99%