Identification of protein phosphatase 2C and confirmation of other protein phosphatases in the ocular lenses

Umeda, Izumi O.; Nakata, H.; Nishigori, Hideo

doi:10.1016/j.exer.2004.06.013

Cited by 8 publications

(2 citation statements)

References 29 publications

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“…Previous research has revealed that the PP1-binding proteins protein phosphatase 1 regulatory subunit 18 (PPP1R18) and PPPIR subunit 3D (PPP1R3D) play a critical role in regulating vertebrate studies of development [ 28 ]. In addition, PP1 plays a key role in both the lens and human retinal epithelium [ 29 ]. PGBD2 is a member of the PiggyBac family [ 30 ], and there are few studies on the relationship between PGBD2 and myopia.…”

Section: Discussionmentioning

confidence: 99%

Identification of potential biomarkers of myopia based on machine learning algorithms

Zhang,

Wang,

Wang

et al. 2023

BMC Ophthalmol

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Purpose This study aims to identify potential myopia biomarkers using machine learning algorithms, enhancing myopia diagnosis and prognosis prediction. Methods GSE112155 and GSE15163 datasets from the GEO database were analyzed. We used “limma” for differential expression analysis and “GO plot” and “clusterProfiler” for functional and pathway enrichment analyses. The LASSO and SVM-RFE algorithms were employed to screen myopia-related biomarkers, followed by ROC curve analysis for diagnostic performance evaluation. Single-gene GSEA enrichment analysis was executed using GSEA 4.1.0. Results The functional analysis of differentially expressed genes indicated their role in carbohydrate generation and polysaccharide synthesis. We identified 23 differentially expressed genes associated with myopia, four of which were highly effective diagnostic biomarkers. Single gene GSEA results showed these genes control the ubiquitin-mediated protein hydrolysis pathway. Conclusion Our study identifies four key myopia biomarkers, providing a foundation for future clinical and experimental validation studies.

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Section: Discussionmentioning

confidence: 99%

Identification of potential biomarkers of myopia based on machine learning algorithms

Zhang,

Wang,

Wang

et al. 2023

BMC Ophthalmol

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“…17,31,[38][39][40] PP1 can also indirectly affect the NKCC/KCC phosphorylation by inhibiting SPAK. 27,35 While the phosphatases PP1 and PP2A are known to be actively expressed in rodent and human lenses, [41][42][43][44] less is known about the regulatory kinases. However, proteomic analysis has identified SPAK in the mouse, 45 and more recently the human lens membrane proteome.…”

mentioning

confidence: 99%

Identification of the WNK-SPAK/OSR1 Signaling Pathway in Rodent and Human Lenses

Vorontsova

Lam

Delpire

et al. 2014

Investigative Ophthalmology & Visual Science

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Our results show that the WNK 1, 3, 4, OSR1, and SPAK signaling system known to play a role in regulating the phosphorylation status, and hence activity of the CCCs in other tissues, is also present in the rat and human lenses. The increased susceptibility of SPAK lenses to opacification suggests that disruption of this signaling pathway may compromise the ability of the lens to control its volume, and its ability to maintain its transparency.

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Heparan sulfate proteoglycans (HSPGs) of the ocular lens

Wishart

Lovicu

2023

Progress in Retinal and Eye Research

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Identification of protein phosphatase 2C and confirmation of other protein phosphatases in the ocular lenses

Cited by 8 publications

References 29 publications

Identification of potential biomarkers of myopia based on machine learning algorithms

Identification of potential biomarkers of myopia based on machine learning algorithms

Identification of the WNK-SPAK/OSR1 Signaling Pathway in Rodent and Human Lenses

Heparan sulfate proteoglycans (HSPGs) of the ocular lens

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