Identification of Prolificacy‐Related Differentially Expressed Proteins from Sheep (Ovis aries) Hypothalamus by Comparative Proteomics

Abstract

Reproduction, as a physiologically complex process, can significantly affect the development of the sheep industry. However, a lack of overall understanding to sheep fecundity has long blocked the progress in sheep breeding and husbandry. In the present study, the aim is to identify differentially expressed proteins (DEPs) from hypothalamus in sheep without FecB mutation in two comparison groups: polytocous (PF) versus monotocous (MF) sheep at follicular phase and polytocous (PL) versus monotocous (ML) sheep at luteal phase. Totally 5058 proteins are identified in sheep hypothalamus, where 22 in PF versus MF, and 39 proteins in PL versus ML are differentially expressed, respectively. A functional analysis is then conducted including Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis to reveal the potential roles of these DEPs. The proteins ENSOARP00000020097, ENSOARP00000006714, growth hormone (GH), histone deacetylase 4 (HDAC4), and 5 -3 exoribonuclease 2 (XRN2) in PF versus MF, and bcl-2-associated athanogene 4 (BAG4), insulin-like growth factor-1 receptor (IGF1R), hydroxysteroid 11-beta dehydrogenase 1 (HSD11B1), and transthyretin (TTR) in PL versus ML appear to modulate reproduction, presumably by influencing the activities of gonadotropin-releasing hormone (GnRH). This study provides an alternative method to identify DEPs associated with sheep prolificacy from the hypothalamus. The mass spectrometry data are available via ProteomeXchange with identifier PXD013822.

Integrative proteomic and phosphoproteomic analysis in the female goat hypothalamus to study the onset of puberty

“…In the present study, an updated proteome of the caprine hypothalamus was generated using TMT combined with LC-MS. We identi ed 5,119 proteins, and quanti ed 5,057. The total number of identi ed proteins was similar to the 5,058 proteins identi ed recently by [27] by using TMT technology, which was substantially more than the 360 proteins identi ed by [30] using the iTRAQ approach in the pig hypothalamus. The difference in the number of identi ed proteins is likely because of the difference in species and because of the iTRAQ and TMT approaches, methodological differences used for peptide enrichment detection, and data processing for protein identi cation and validation.…”

“…The TMT-based proteomics and phosphoproteomics strategy is the powerful technique for the global analysis of signaling networks in de ned biological systems [25,26]. In the eld of livestock reproduction, proteomics has been used to determine the molecular basis of hypothalamic function [27], sperm performance [28],…”

RNAseq and quantitative proteomic analysis of Dictyostelium knock-out cells lacking the core autophagy proteins ATG9 and/or ATG16

“…The PRM method was applied to validate the accuracy of the TMT results and was done by Shanghai Applied Protein Technology Co., Ltd [ 47 , 48 ]. Briefly, peptides were prepared according to the TMT protocol, and an AQUA stable isotope peptide was spiked in each sample as internal standard reference.…”

Proteomic Analysis Identifies Distinct Protein Patterns for High Ovulation in FecB Mutant Small Tail Han Sheep Granulosa Cells

“…The log2-transformed and normalized abundance of filtered proteins were calculated using the linear models for microarray data (LIMMA v 3.52.1), employing a factorial design with genotype as a factor ( WW , WB , and BB ) [ 28 ]. For screening differentially abundant proteins (DAPs) between the two groups, the criteria used were a fold change greater than 1.2 or less than 0.83 and a p value lower than 0.05, as explained in a previous study [ 29 ].…”