Identification of prolargin expression in articular cartilage and its significance in rheumatoid arthritis pathology

Pillai, Vinod Soman; Kundargi, Rameshwari R.; Edathadathil, Fabia; Nair, Shantikumar V.; Thilak, Jai; Mathew, Roshini Anney; Xavier, Tessy; Shenoy, Padmanabha; Menon, Krishnakumar N.

doi:10.1016/j.ijbiomac.2018.01.141

Cited by 5 publications

(2 citation statements)

References 29 publications

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“…DPT is reported in chondrocyte culture [ 62 ] where its function might be connected to collagen II in maintaining the mechanical strength or elasticity of the cartilage and that is why it is also a component of the collagen‐containing extracellular matrix. PRELP is expressed in articular cartilage [ 63 ] and the TGF‐β family is known to play a role during chondrogenesis in vitro by committing hBMSCs to mesenchymal chondroprogenitor cells. [ 64 ] Interestingly, within the ECM proteins, also S100A10, S100A9, and S100A7 were upregulated.…”

Section: Resultsmentioning

confidence: 99%

A Proteomic Approach to Determine Stem Cell Skeletal Differentiation Signature on Additive Manufactured Scaffolds

Tomasina,

Mohren,

Camarero‐Espinosa

et al. 2024

Small Science

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Understanding how porous biomaterials interact with cells at their surface and how they either promote or inhibit cellular processes has presented several challenges. Additive manufacturing enables the fabrication of scaffolds with distinct compositions and designs for different tissue engineering applications. To evaluate the in vitro performance of multiple printed materials, biochemical assays can be limiting in providing valuable insight and key information to select the best tissue destination. Omics technologies like proteomics are crucial for studying important cellular events and gathering valuable information about cellular processes and mechanisms. However, only few studies focus on proteomics to decipher cell–material interactions and cell differentiation on additive manufactured scaffolds. Here, scaffolds were fabricated using three polymers (polycaprolactone (PCL), poly(ethylene oxide)–poly(butylene terephthalate) (PEOT/PBT), and polylactic acid (PLA)) through additive manufacturing. Their chondrogenic and osteogenic potential were characterized and compared using human bone marrow‐derived mesenchymal stem cells (hBMSCs) through proteomics analysis. The 3D scaffolds were all hydrophilic and displayed Young's moduli close to those of bone or cartilage for PLA and PCL and PEOT/PBT, respectively. Biochemical assays indicated that PEOT/PBT and PLA scaffolds have a greater chondrogenic potential by higher glycosaminoglycan (GAG) and collagen deposition compared to PCL. PLA and PEOT/PBT showed to be more effective in promoting bone formation, as evidenced by higher calcium deposits detected by alizarin red staining, and higher alkaline phosphatase (ALP), especially for PLA in osteogenic medium. Proteomics data revealed the most distinct separation between conditions in chondrogenic medium, which had the highest protein identification rates. Pathway analysis showed that PCL did not induce any differentiation‐related pathways when compared to PEOT/PBT and PLA in any of the tested media conditions. Analysis of PEOT/PBT proteins showed pathways involved in chondrogenesis in all three media and pathways related to hypertrophic phenotype progression in chondrogenic medium. These data suggests that PEOT/PBT is a valuable candidate for cartilage and osteochondral applications, able to drive hBMSCs differentiation without the need of growth factors. PLA was also a valuable candidate for cartilage and bone applications by upregulating both chondrogenic and osteogenic‐related proteins in maintenance and chondrogenic media. In osteogenic and maintenance media, the upregulation of angiogenic proteins makes PLA a better candidate for bone application where vascularization is key.

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Section: Resultsmentioning

confidence: 99%

A Proteomic Approach to Determine Stem Cell Skeletal Differentiation Signature on Additive Manufactured Scaffolds

Tomasina,

Mohren,

Camarero‐Espinosa

et al. 2024

Small Science

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“…Two dimensional gel electrophoresis (2-DE) was carried out as per the method given by Vinod et al (2018). An amount of 200 µg of boiled sample was made upto 125 µl using rehydration buffer having a final concentration of 7 M urea, 2 Mthiourea, 2% DTT, 2% ASB-14-4, 4% CHAPS, 0.001% bromophenol blue and 2% ampholyte (pH 3-10).…”

Section: D Gel Electrophoresis and Immunoblottingmentioning

confidence: 99%

Effect of extended period of boiling on allergic protein of flower tail shrimp Metapenaeus dobsoni

Laly

Sankar²,

Panda³

2019

Indian J. Fish.

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Consumption of shellfishes is a major cause of allergic reaction and IgE mediated hypersensitivity is an increasing health issue in sensitive individuals. Thermal stability of allergen of the flower tail shrimp Metapenaeus dobsoni was evaluated during extended period of boiling for immunoreactivity. Shrimp extracts boiled for 5 to 25 min showed increased IgE reactivity of 0.251 to 0.268 at absorbance of 490 nm in comparison to raw extract. Trpomyosin of 37 KDa was observed to withstand longer periods of boiling and found to retain IgE binding activity. Thermal treatment by means of boiling for longer duration increased the recognition of this major allergen in individuals with shrimp sensitivity. Hence, tropomyosin subjected to longer period of boiling could be used in facilitating shrimp allergy diagnosis and for detection of allergen in processed food products.

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Regulation of Cartilage Matrix Protein by Transcription Factors, SOX9 and β-Catenin

Chen

2020

Encyclopedia of Bone Biology

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Identification of prolargin expression in articular cartilage and its significance in rheumatoid arthritis pathology

Cited by 5 publications

References 29 publications

A Proteomic Approach to Determine Stem Cell Skeletal Differentiation Signature on Additive Manufactured Scaffolds

A Proteomic Approach to Determine Stem Cell Skeletal Differentiation Signature on Additive Manufactured Scaffolds

Effect of extended period of boiling on allergic protein of flower tail shrimp Metapenaeus dobsoni

Regulation of Cartilage Matrix Protein by Transcription Factors, SOX9 and β-Catenin

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