Identification of potentially virulent strains of Haemophilus parasuis using a multiplex PCR for virulence-associated autotransporters (vtaA)

Olvera, Àlex; Pina, Sonia; Macedo, Núbia; Oliveira, Simone; Aragón, Virginia; Bensaid, Albert

doi:10.1016/j.tvjl.2010.12.014

Cited by 37 publications

(51 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, all strains were isolated from lungs of swine with clinical signs of Glasser´s disease and the frequency of vtaA1 gene was high as could be expected (86%). Olvera et al, (19), also describe the occurrence of vtaA1 negative isolates from lungs as observed in seven strains examined here. In some cases were selected more than one isolate from the same animal, and was observed that six of seven vtaA1 negative strains were isolated from animals also carrying vtaA1positive H. parasuis strains ( Figure 1-profiles 1, 3, 4 and 11).…”

Section: Serotyping and Pcrsupporting

confidence: 69%

“…Strains from different serovars were identified on specimens from the same animal and same farm, as showed by Table 1. All tested strains were positive to species-specific amplification of the 16S rRNA gene as described by Oliveira et al (12) and to Group 3 vtaA gene described by Olvera et al (19). From 50 strains tested 43 (86%) were positive to Group 1 vtaA gene.…”

Section: Serotyping and Pcrmentioning

confidence: 85%

“…ranging from 80% to 100% of similarity, 9 of which were nontypeable by serotyping. Olvera et al (19) developed a PCR to characterize trimeric autotransporters genes (vtaA) associated to virulence, they describe that group 3 vtaA gene seems to be conserved among invasive and non-invasive strains and may be used to species identification, while group 1 vtaA gene showed better correlation with virulent strains. In the present study, all strains were isolated from lungs of swine with clinical signs of Glasser´s disease and the frequency of vtaA1 gene was high as could be expected (86%).…”

Section: Serotyping and Pcrmentioning

confidence: 99%

See 2 more Smart Citations

ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine

Moreno

Castilla

Gobbi

et al. 2011

Braz. J. Microbiol.

View full text Add to dashboard Cite

Haemophilus parasuis infection, known as Glässer's disease, is characterized by fibrinous polyserositis, arthritis and meningitis in piglets. Although traditional diagnosis is based on herd history, clinical signs, bacterial isolation and serotyping, the molecular-based methods are alternatives for species-specific tests and epidemiologic study. The aim of this study was to characterize H. parasuis strains isolated from different states of Brazil by serotyping, PCR and ERIC-PCR. Serotyping revealed serovar 4 as the most prevalent (24 %), followed by serovars 14 (14 %), 5 (12 %), 13 (8 %) and 2 (2 %), whereas 40 % of the strains were considered as non-typeable. From 50 strains tested 43 (86%) were positive to Group 1 vtaA gene that have been related to virulent strains of H.parasuis. ERIC-PCR was able to type isolates tested among 23 different patterns, including non-typeable strains. ERIC-PCR patterns were very heterogeneous and presented high similarity between strains of the same animal or farm origin. The results indicated ERIC-PCR as a valuable tool for typing H. parasuis isolates collected in Brazil.

show abstract

Section: Serotyping and Pcrsupporting

confidence: 69%

Section: Serotyping and Pcrmentioning

confidence: 85%

Section: Serotyping and Pcrmentioning

confidence: 99%

See 1 more Smart Citation

ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine

Moreno

Castilla

Gobbi

et al. 2011

Braz. J. Microbiol.

View full text Add to dashboard Cite

show abstract

“…H. parasuis identification was also confirmed by PCR based on species-specific amplification of the 16S rRNA gene, as described by Oliveira et al [7]. All isolates were also positive for the amplification of the group 1 vtaA gene [8] that has been associated with virulent H. parasuis strains.…”

mentioning

confidence: 70%

Application of protein profiling of virulent Haemophilus parasuis by MALDI-TOF mass spectrometry

Moreno

Silva

Gomes

et al. 2016

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…However, serovar is regarded as a poor proxy for virulence, with the exception of serovars 4 and 5 (12)(13)(14). The vtaA multiplex PCR (mPCR) (15)(16)(17) is the only method that has shown an association between the test result for a given isolate and its virulence, but its wide-scale use has not been reported.…”

mentioning

confidence: 99%

“Pathotyping” Multiplex PCR Assay for Haemophilus parasuis: a Tool for Prediction of Virulence

et al. 2017

Self Cite

View full text Add to dashboard Cite

Haemophilus parasuis is a diverse bacterial species that is found in the upper respiratory tracts of pigs and can also cause Glässer's disease and pneumonia. A previous pangenome study of H. parasuis identified 48 genes that were associated with clinical disease. Here, we describe the development of a generalized linear model (termed a pathotyping model) to predict the potential virulence of isolates of H. parasuis based on a subset of 10 genes from the pangenome. A multiplex PCR (mPCR) was constructed based on these genes, the results of which were entered into the pathotyping model to yield a prediction of virulence. This new diagnostic mPCR was tested on 143 field isolates of H. parasuis that had previously been whole-genome sequenced and a further 84 isolates from the United Kingdom from cases of H. parasuis-related disease in pigs collected between 2013 and 2014. The combination of the mPCR and the pathotyping model predicted the virulence of an isolate with 78% accuracy for the original isolate collection and 90% for the additional isolate collection, providing an overall accuracy of 83% (81% sensitivity and 93% specificity) compared with that of the "current standard" of detailed clinical metadata. This new pathotyping assay has the potential to aid surveillance and disease control in addition to serotyping data.

show abstract

Identification of potentially virulent strains of Haemophilus parasuis using a multiplex PCR for virulence-associated autotransporters (vtaA)

Cited by 37 publications

References 38 publications

ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine

ERIC-PCR genotypic characterization of Haemophilus parasuis isolated from Brazilian swine

Application of protein profiling of virulent Haemophilus parasuis by MALDI-TOF mass spectrometry

“Pathotyping” Multiplex PCR Assay for Haemophilus parasuis: a Tool for Prediction of Virulence

Contact Info

Product

Resources

About