Identification of Potentially Related Genes and Mechanisms Involved in Skeletal Muscle Atrophy Induced by Excessive Exercise in Zebrafish

Sun, Chaofen; Zhou, Zuoqiong; Chen, Zhanglin; Zhu, Runkang; Yang, Dong Kwon; Peng, Xiyang; Zheng, Lan; Tang, Cui

doi:10.3390/biology10080761

Cited by 8 publications

(8 citation statements)

References 56 publications

(59 reference statements)

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“…In rainbow trout (O. mykiss), it was determined that infectious pancreatic necrosis virus (IPNV) infection induced a dynamic response between autophagy and the proteasomal pathways in skeletal muscle [45]. Furthermore, autophagymediated skeletal muscle atrophy has been found to be induced by intensive exercise in zebrafish (Danio rerio) [46]. Among the genes with differential expression validated by RT-qPCR are mapk10, bnip3, atg9a, and raf1.…”

Section: Discussionmentioning

confidence: 99%

High-Temperature Stress Induces Autophagy in Rainbow Trout Skeletal Muscle

Molina

Dettleff

Valenzuela-Muñoz

et al. 2023

Fishes

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Ectothermic animals, such as teleosts, have increasingly been exposed to stressful high-temperature events due to global warming. Currently, the effects of thermal stress on skeletal muscle, a key tissue for fish growth, are unknown. This study examined the impact of high-temperature stress on the skeletal muscle transcriptome of rainbow trout (Oncorhynchus mykiss) in control (15 °C) and high-temperature (20 °C) conditions. Additionally, we examined the plasmatic levels of cortisol, glucose, and creatine kinase activity, and examined oxidative damage and autophagy activation in skeletal muscle. High-temperature stress induced significant increases in cortisol and glucose plasmatic levels. Nevertheless, no changes were observed in creatine kinase activity in plasma and skeletal muscle oxidation. Skeletal muscle RNA was isolated and sequenced using the HiSeq Illumina platform. A total of 383,796,290 reads were mapped onto the reference rainbow trout genome. The transcriptomic analysis showed that 293 genes were upregulated in the high-temperature group, mainly associated with autophagosome assembly, amino acid transport, and the glutamine metabolic process. On the other hand, 119 genes were downregulated in the high-temperature group, mainly associated with digestion, proteolysis, and the muscle contraction process. In addition, RT-qPCR of differentially expressed representative genes and Western blot analysis of LC3-II/LC3-I levels confirmed skeletal muscle autophagy induced by high temperature. This study sheds light on intriguing facets of the adaptive response of rainbow trout skeletal muscle to high-temperature stress and provides significant insights into the physiology of autophagy in teleosts.

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Section: Discussionmentioning

confidence: 99%

High-Temperature Stress Induces Autophagy in Rainbow Trout Skeletal Muscle

Molina

Dettleff

Valenzuela-Muñoz

et al. 2023

Fishes

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“…We measured MO 2 and Ucrit in three zebrafish groups based on previous descriptions [54]. In brief, zebrafish were acclimatized to the Loligo® system for 30 min, and parameters were set according to their body weight and length until the zebrafish were swimming to exhaustion.…”

Section: Methodsmentioning

confidence: 99%

“…The methods used for total RNA extraction, cDNA generation, and quantitative reverse transcription polymerase chain reaction of the zebrafish skeletal muscle have been described previously [54]. In addition, gene expression levels were normalized to the GAPDH levels using the 2 −ΔΔCt method.…”

Section: Methodsmentioning

confidence: 99%

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Exercise intervention mitigates zebrafish age‐related sarcopenia via alleviating mitochondrial dysfunction

et al. 2022

Self Cite

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Sarcopenia is a common disorder that leads to a progressive decrease in skeletal muscle function in elderly people. Exercise effectively prevents or delays the onset and progression of sarcopenia. However, the molecular mechanisms underlying how exercise intervention improves skeletal muscle atrophy remain unclear. In this study, we found that 21-month-old zebrafish had a decreased swimming ability, reduced muscle fibre cross-sectional area, unbalanced protein synthesis, and degradation, increased oxidative stress, and mitochondrial dysfunction, which suggests zebrafish are a valuable model for sarcopenia. Eight weeks of exercise intervention attenuated these pathological changes in sarcopenia zebrafish. Moreover, the effects of exercise on mitochondrial dysfunction were associated with the activation of the AMPK/SIRT1/PGC-1a axis and 15-PGDH downregulation. Our results reveal potential therapeutic targets and indicators to treat agerelated sarcopenia using exercise intervention.

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“…It is important to acknowledge that using older individuals may yield different results. Additionally, studies have shown that excessive exercise can result in muscle atrophy and an increase in the expression of Atrogin-1 and MuRF1 in zebrafish muscle [19]. Cold-reared zebrafish can be valuable for studying the ideal intensity and duration of exercise needed to prevent muscle atrophy.…”

Section: Effect Of Forced Swimming On Gene Expression Related To Musc...mentioning

confidence: 99%

Cold-induced muscle atrophy in zebrafish: Insights from swimming activity and gene expression analysis

Ikeda,

Fujita,

Toda

et al. 2023

Preprint

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The investigation into the effects of cold acclimation on fish skeletal muscle function and its potential implications for muscle atrophy is of great interest to us. This study examines how rearing zebrafish at low temperatures affects their locomotor activity and the expression of genes associated with muscle atrophy. Zebrafish were exposed to temperatures ranging from 10 °C to 25 °C, and their swimming distance was measured. The expression levels of important muscle atrophy genes, Atrogin-1 and MuRF1, were also evaluated. Our findings show that swimming activity significantly decreases when the water temperature ranges from 10 °C to 15 °C, indicating a decrease in voluntary movement. Additionally, gene expression analysis shows a significant increase in the expression of Atrogin-1 and MuRF1 at 10 °C. This up-regulation could lead to muscle atrophy caused by decreased activity in cold temperatures. To investigate the effects of exercise on reducing muscle atrophy, we subjected zebrafish to forced swimming at a temperature of 8 °C for ten days. This treatment significantly reduced the expression of Atrogin-1 and MuRF1, emphasizing the importance of muscle stimulation in preventing muscle atrophy in zebrafish. These findings suggest that zebrafish can serve as a valuable model organism for studying muscle atrophy and can be utilized in drug screening for muscle atrophy-related disorders. Cold-reared zebrafish provide a practical and ethical approach to inducing disuse muscle atrophy, providing valuable insights into potential therapeutic strategies for addressing skeletal muscle atrophy.

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Identification of Potentially Related Genes and Mechanisms Involved in Skeletal Muscle Atrophy Induced by Excessive Exercise in Zebrafish

Cited by 8 publications

References 56 publications

High-Temperature Stress Induces Autophagy in Rainbow Trout Skeletal Muscle

High-Temperature Stress Induces Autophagy in Rainbow Trout Skeletal Muscle

Exercise intervention mitigates zebrafish age‐related sarcopenia via alleviating mitochondrial dysfunction

Cold-induced muscle atrophy in zebrafish: Insights from swimming activity and gene expression analysis

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