Identification of potential miRNA-mRNA regulatory network and the key miRNAs in intramuscular and subcutaneous adipose

Abstract

Intramuscular fat (IMF) is an important indicator for evaluating meat quality. Breeds with high IMF content are often accompanied by high subcutaneous fat (SCF), severely affecting the meat rate of pigs. Studying the mechanisms of miRNAs related to lipogenesis and lipid metabolism has important implications for pig breeding. We constructed two small RNA libraries from intramuscular and subcutaneous fat to evaluate the patterns of lipogenesis in Laiwu pig, a Chinese breed. A total of 286 differentially expressed miRNAs (DEmiRNAs), including 193 known miRNA and 93 novel miRNAs, were identified from two types of adipose. GO and KEGG enrichment analysis for DEmiRNAs showed that their target genes involved in many adipogenesis and lipid metabolism biological processes and signaling pathways, such as Wnt signaling pathway,MAPK signaling pathway, Hippo signaling pathway, PI3K-Akt signaling pathway, Melanogenesis, Signaling pathways regulating pluripotency of stem cells and so on. Then, we constructed a miRNA-mRNA interaction network to find out which miRNAs were the key miRNAs of regulation in Wnt signaling pathway. In this pathway, miR-331-3p, miR-339-5p, miR-874 and novel346_mature target PPARD, WNT10B, RSPO3, WNT2B. This study provides a theoretical basis for further understanding the post-transcriptional regulation mechanism of meat quality formation and predicting and treating diseases caused by ectopic fat.

Comparative analysis of alternative splicing events in skeletal muscle of different sheep

“…In this study, the sheep transcriptome was screened for differential AS event through a deep sequencing-based approach, the sequencing-by-synthesis (SBS) technique offered by Illumina platforms. This has been proven to be a highly accurate means of sequencing [ [10] , [11] , [12] , [13] , [25] , [26] , [27] , [28] ]. We selected the Han and Dorset sheep in this study because of their significantly different growth rates and fat content.…”

Identification of Differentially Expressed miRNAs in Porcine Adipose Tissues and Evaluation of Their Effects on Feed Efficiency

“…Overexpression of miR-196a can increase lipid accumulation and promote preadipocyte differentiation [ 93 ]. miR-331, a microRNA participating in regulating the proliferation, differentiation and fatty-acid accumulation of porcine preadipocytes, was up-regulated in the adipose tissues of high-FE pigs [ 94 ]. Overexpressing miR-331 can increased fatty-acid synthesis [ 95 ].…”

A review of the role of transcription factors in regulating adipogenesis and lipogenesis in beef cattle

“…Regarding the role of transcription factors and miRNAs, beef cattle, sheep and pigs all involve PPARγ, miRNAs and other transcription factors in regulating lipid metabolism and IMF content (Duckett & Greene, 2022; Feng et al., 2022; Han et al., 2021). While these commonalities exist, there are differences in how these factors operate in each species.…”