Identification of potential miRNA biomarkers for traumatic osteonecrosis of femoral head

Abstract

Traumatic osteonecrosis of femoral head (TONFH) is a common orthopedic disease caused by physical injury in hip. However, the unclear pathogenesis mechanism of TONFH and lacking of simple noninvasive early diagnosis method cause the necessity of hip replacement for most patients with TONFH. In this study, we aimed to identify circulating microRNAs (miRNAs) by integrated bioinformatics analyses as potential biomarker of TONFH. mRNA expression profiles were downloaded from the Gene Expression Omnibus database. Then we combined two miRNA screen methods: Weighted gene co‐expression network analysis and fold change based differentially expressed miRNAs analysis. As a result, we identified 14 key miRNAs as potential biomarkers for TONFH. Besides, 302 target genes of these miRNAs were obtained and the miRNA–mRNA interaction network was constructed. Furthermore, the results of Kyoto Encyclopedia of Gene and Genome pathway analysis, Gene Ontology function analysis, protein–protein interaction (PPI) network analysis and PPI network module analysis showed close correlation between these 14 key miRNAs and TONFH. Then we established receiver operating characteristic curves and identified 6‐miRNA signature with highly diagnosis value including miR‐93‐5p (area under the curve [AUC] = 0.93), miR‐1324 (AUC = 0.92), miR‐4666a‐3p (AUC = 0.92), miR‐5011‐3p (AUC = 0.92), and miR‐320a (AUC = 0.89), miR‐185‐5p (AUC = 0.89). Finally, the results of quantitative real‐time polymerase chain reaction confirmed the significantly higher expression of miR‐93‐5p and miR‐320a in the serum of patients with ONFH. These circulating miRNAs could serve as candidate early diagnosis markers and potential treatment targets of TONFH.

Construction of Immune-related lncRNA Prognosis Models and Development Related Networks and Drugs in LGG

“…It is another validation of the effectiveness of our lncRNA network. MIR4666A has been reported to have a possible role in femoral necrosis [47] , atrial brillation [48] , and congestive heart failure [49] , but the present study suggests a possible role in glioma immunity and prognosis. AC093726 also performed well in prognostic and immunological analyses, suggesting a possible association with tumor development.…”

Differentially expressed serum proteins in children with or without asthma as determined using isobaric tags for relative and absolute quantitation proteomics

“…GO is useful for comprehensively describing the attributes of genes and gene products in organisms. GO consists of three domains (Liu et al, 2020;Wang et al, 2019a;Xing et al, 2020): (1) the cellular component domain contains the descriptions of proteins related to cell composition, which may be a subcellular structure (e.g., endoplasmic reticulum or nucleus) or a protein production component (e.g., ribosome, or proteasome); (2) the molecular function domain contains descriptions of all proteins related to molecular functions, such as biological activities and operations performed by specific gene products (i.e., molecules or complexes); (3) the biological process domain contains descriptions of all proteins related to biological processes, that is, a series of events in which molecular functions cooperate with one another. The GO function annotation result refers to the statistical number of DEPs detected between the two groups of serum samples in the three GO domains.…”

Peer Review #1 of "Differentially expressed serum proteins in children with or without asthma as determined using isobaric tags for relative and absolute quantitation proteomics (v0.1)"

“…GO is useful for comprehensively describing the attributes of genes and gene products in organisms. GO consists of three domains (Liu et al 2020;Wang et al 2019a;Xing et al 2020): (1) the cellular component domain contains the descriptions of proteins related to cell composition, which may be a subcellular structure (e.g., endoplasmic reticulum or nucleus) or a protein production component (e.g., ribosome, or proteasome); (2) the molecular function domain contains descriptions of all proteins related to molecular functions, such as biological activities and operations performed by specific gene products (i.e., molecules or complexes); (3) the biological process domain contains descriptions of all proteins related to biological processes, that is, a series of events in which molecular functions cooperate with one PeerJ reviewing PDF | (2020:03:47037:2:0:NEW 22 Aug 2020)…”