Identification of polyunsaturated fatty acid and diterpenoid biosynthesis pathways from draft genome of Aureispira sp. CCB-QB1

Furusawa, Go; Lau, Nyok-Sean; Shu‐Chien, Alexander Chong; Jaya-Ram, Annette; Amirul, Al-Ashraf Abdullah

doi:10.1016/j.margen.2014.10.006

Cited by 19 publications

(21 citation statements)

References 41 publications

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“…CCB-QB1 (to be called Aureispira QB1) was isolated from Queens Bay, Penang, Malaysia (Furusawa et al, 2015). During the enrichment and purification process, white coloured swarming colonies, reminiscent of Vibrio sp., were observed on the low nutrient artificial seawater medium (L-ASWM) (Furusawa et al, 2015) agar plates (0.05 % tryptone, 2.4 % artificial seawater mix, 1.5 % agar, 10 mM HEPES, final pH 7.6) alongside the swarms of Aureispira. The Vibrio sp.…”

Section: Methodsmentioning

confidence: 99%

“…cells was prepared using the GNOME DNA kit (MP Biomedical). Phylogenetic analysis based on 16S rRNA gene sequencing was described previously (Furusawa et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

“…were maintained on high-nutrient artificial seawater agar medium (H-ASWM) (0.5 % tryptone, 2.4 % artificial seawater mix, 1.5 % agar, 10 mM HEPES, pH 7.6) (Furusawa et al, 2015). Aureispira QB1 cells were grown to a density of 1|10 8 cells ml 21 (OD 600 51) in H-ASWM at 30 uC overnight.…”

Section: Methodsmentioning

confidence: 99%

“…In 2014, the draft genome sequence of Aureispira QB1 was determined and annotated (Furusawa et al, 2015). Genes homologous to Flavobacterium psychrophilum JIP02/86 alg were found using the Rapid Annotation using Subsystem Technology (RAST) server and SEED-Viewer (Aziz et al, 2008).…”

Section: Methodsmentioning

confidence: 99%

“…In order to achieve a better understanding of the mechanism of ixotrophy, conditions that support ixotrophy in Aureispira QB1, a member of the family Saprospiraceae (Furusawa et al, 2015), were investigated. Aureispira QB1 aggregated quickly in artificial seawater (ASW) and was able to prey on Vibrio sp.…”

Section: Introductionmentioning

confidence: 99%

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Calcium is required for ixotrophy of Aureispira sp. CCB-QB1

2015

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Ixotrophy is a process that enables certain microbes to prey on other cells. The ability of cells to aggregate or adhere is thought to be a significant initial step in ixotrophy. The gliding, multicellular filamentous bacterium Aureispira sp. CCB-QB1 belongs to the family Saprospiraceae and preys on bacteria such as Vibrio sp. in seawater. Adhesion and cell aggregation were coincident with preying and were hypothesized to play an important role in the ixotrophy in this bacterium. To test this hypothesis, experiments to elucidate the mechanisms of aggregation or adhesion in this bacterium were performed. The ability of Aureispira QB1 to adhere and aggregate to prey bacterium, Vibrio sp., required divalent cations, especially calcium ions. In the presence of calcium, Aureispira QB1 cells captured 99 % of Vibrio sp. cells after 60 min of incubation. Toluidine blue O, which binds acidic polysaccharides, bound to Aureispira QB1 and inhibited adhesion of Aureispira QB1. These results suggest that acidic polysaccharides are needed for aggregation or adhesion of Aureispira and that calcium ions play a significant role in these phenomena.

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Section: Methodsmentioning

confidence: 99%

“…cells was prepared using the GNOME DNA kit (MP Biomedical). Phylogenetic analysis based on 16S rRNA gene sequencing was described previously (Furusawa et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Calcium is required for ixotrophy of Aureispira sp. CCB-QB1

2015

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Comparative analysis of bacteria associated with different mosses by 16S rRNA and 16S rDNA sequencing

Tian

2016

J. Basic Microbiol.

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To understand the differences of the bacteria associated with different mosses, a phylogenetic study of bacterial communities in three mosses was carried out based on 16S rDNA and 16S rRNA sequencing. The mosses used were Hygroamblystegium noterophilum, Entodon compressus and Grimmia montana, representing hygrophyte, shady plant and xerophyte, respectively. In total, the operational taxonomic units (OTUs), richness and diversity were different regardless of the moss species and the library level. All the examined 1183 clones were assigned to 248 OTUs, 56 genera were assigned in rDNA libraries and 23 genera were determined at the rRNA level. Proteobacteria and Bacteroidetes were considered as the most dominant phyla in all the libraries, whereas abundant Actinobacteria and Acidobacteria were detected in the rDNA library of Entodon compressus and approximately 24.7% clones were assigned to Candidate division TM7 in Grimmia montana at rRNA level. The heatmap showed the bacterial profiles derived from rRNA and rDNA were partly overlapping. However, the principle component analysis of all the profiles derived from rDNA showed sharper differences between the different mosses than that of rRNA-based profiles. This suggests that the metabolically active bacterial compositions in different mosses were more phylogenetically similar and the differences of the bacteria associated with different mosses were mainly detected at the rDNA level. Obtained results clearly demonstrate that combination of 16S rDNA and 16S rRNA sequencing is preferred approach to have a good understanding on the constitution of the microbial communities in mosses.

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An assimilatory sulfite reductase, CysI, negatively regulates the dormancy of Microbulbifer aggregans CCB‐MM1^T

Diyana

Furusawa

2021

J Basic Microbiol

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Sulfur is one of the common and essential elements of all life. Sulfate, which is a major source of sulfur, plays an important role in synthesizing sulfur‐containing amino acids, such as cysteine and methionine, organic compounds essential to all living organisms. Some investigations reported that the assimilatory sulfate reduction pathway (ASRP) involved in cysteine synthesis is crucial to entering bacterial dormancy in pathogens. Our previous investigation reported that the halophilic marine bacterium, Microbulbifer aggregans CCB‐MM1T, possesses an ASRP and the dissimilatory sulfate reduction pathway (DSRP). The bacterium might use DSRP to generate energy required for entering its dormant. However, the role of the ASRP in the dormancy of M. aggregans CCB‐MM1T was so far unknown. In this study, we found that genes involved in ASRP were downregulated in the dormancy. The disruption of the gene encoding an assimilatory sulfite reductase, cysI, suppressed a completely dormant state under low nutrient conditions. In addition, the cysI mutant showed cell aggregation at the middle‐exponential phase under high nutrient conditions, indicating that the mutation might be stimulated to enter the dormancy. The wild‐type phenotype of the bacterium was recovered by the addition of cysteine. These results suggested that cysteine concentration may play an important role in inducing the dormancy of M. aggregans.

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Identification of polyunsaturated fatty acid and diterpenoid biosynthesis pathways from draft genome of Aureispira sp. CCB-QB1

Cited by 19 publications

References 41 publications

Calcium is required for ixotrophy of Aureispira sp. CCB-QB1

Calcium is required for ixotrophy of Aureispira sp. CCB-QB1

Comparative analysis of bacteria associated with different mosses by 16S rRNA and 16S rDNA sequencing

An assimilatory sulfite reductase, CysI, negatively regulates the dormancy of Microbulbifer aggregans CCB‐MM1^T

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Identification of polyunsaturated fatty acid and diterpenoid biosynthesis pathways from draft genome of Aureispira sp. CCB-QB1

Cited by 19 publications

References 41 publications

Calcium is required for ixotrophy of Aureispira sp. CCB-QB1

Calcium is required for ixotrophy of Aureispira sp. CCB-QB1

Comparative analysis of bacteria associated with different mosses by 16S rRNA and 16S rDNA sequencing

An assimilatory sulfite reductase, CysI, negatively regulates the dormancy of Microbulbifer aggregans CCB‐MM1T

Contact Info

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An assimilatory sulfite reductase, CysI, negatively regulates the dormancy of Microbulbifer aggregans CCB‐MM1^T