Identification of Polyphenolics from Loranthus globosus as Potential Inhibitors of Cholinesterase and Oxidative Stress for Alzheimer’s Disease Treatment

Abstract

Mistletoes are considered to be the potential medicinal herbs due to their rich traditional uses. Loranthus globosus is a Bangladeshi mango mistletoe that has been reported as folk medicine for various ailments and diseases. In an attempt to explore its effectiveness in Alzheimer’s disease (AD), we investigated the antioxidant and acetylcholinesterase inhibitory activity of L. globosus. We report that the crude methanol extract (CME) of the plant contains a good amount of polyphenolics and possesses antioxidant and cholinesterase inhibitory activity. Fractionation of CME with solvents of varying polarity revealed the highest activity and polyphenolic content in the ethylacetate fraction (EAF). Correlation analysis revealed a significant ( P < 0.05 ) association of polyphenolics with the antioxidant and cholinesterase inhibitory properties. Using column chromatography with diaion resin, the polyphenolics (EAF-PP) were isolated from the EAF that displayed the potent antioxidant and cholinesterase inhibitory activities. Kinetic analysis showed that EAF-PP exhibited a competitive type of inhibition. A total of thirty-six compounds including catechin and its different derivatives were identified in the EAF-PP by LC/MS analysis. Bioactivity-guided separation approach afforded the isolation of the two major active compounds catechin and catechin dimer from the EAF-PP. Hence, EAF-PP represents a potential source of antioxidants and cholinesterase inhibitors, which can be used in the management of AD.

Compounds from the Petroleum Ether Extract of Wedelia chinensis with Cytotoxic, Anticholinesterase, Antioxidant, and Antimicrobial Activities

“…The excessively generated reactive oxygen species causes oxidation of protein, lipid, and DNA which play a role in the pathogenesis of the diseases [ 30 ]. Antioxidants from plants have been found to effectively inhibit the oxidation and oxidative damage in the oxidative induced diseases [ 31 ]. In this study, we found that all the compounds have antioxidant activity in terms of reducing power.…”

Antimicrobial Resistance: A Growing Serious Threat for Global Public Health

“…Plants are being considered as untapped sources of potential antimicrobial agents, according to recent findings on compounds derived from plants, such polyphenolics, alkaloids, and other plant extracts [ 80 ]. However, the antimicrobial potential of several phytochemical compounds including polyphenolics, alkaloids, and flavonoids has yet to be investigated [ 81 , 82 , 83 , 84 , 85 , 86 ]. Moreover, the big challenge is to transfer and translate some of these precious discoveries out of laboratories and into hospital practices.…”

Combating antimicrobial resistance: the silent war

“…Plants are recognized as a great resource of antimicrobial agents ( Abd El-Hack et al, 2022 ; Al-Amin et al, 2022 ). A variety of compounds originated from plants with potent antimicrobial activities, such as alkaloids, polyphenolics, flavonoids, and certain plant extracts ( Islam et al, 2021 ; Kundo et al, 2021 ; Foyzun et al, 2022 ; Pawar et al, 2022 ). Although several phytochemical compounds have been identified in the research, there remain numerous compounds that require further investigation.…”