Identification of plasmid-mediated extended-spectrum and AmpC β-lactamases in Enterobacter spp. isolated from dogs

Sidjabat, Hanna E.; Hanson, Nancy D.; Smith-Moland, Ellen; Bell, Jan M.; Gibson, Justine S.; Filippich, L. J.; Trott, Darren J.

doi:10.1099/jmm.0.46888-0

Cited by 42 publications

(43 citation statements)

References 37 publications

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“…Extended-spectrum cephalosporin resistance was encoded by bla CMY-2 and bla DHA-1 genes, which have been previously reported in other animals and other bacterial species in Australia (Abraham et al, 2014;Cherif et al, 2015;Rogers et al, 2014;Sidjabat et al, 2007), but not previously in poultry. The bla CMY-2 gene is the most frequently detected AmpC β-lactamase in food-producing animals worldwide (Borjesson et al, 2013;Bortolaia et al, 2010;Cherif et al, 2015;El-Shazly et al, 2017;Ewers et al, 2012;Jacoby, 2009 Lee et al, 2010;Maamar et al, 2016;Mataseje et al, 2010;Pacholewicz et al, 2015;Rasmussen et al, 2015;Rayamajhi et al, 2010;Rayamajhi et al, 2008;Tagg et al, 2015;Yan et al, 2006).…”

Section: Discussionmentioning

confidence: 93%

“…The bla CMY-2 and bla DHA-1 genes have been previously reported in other animals and other bacterial species in Australia (Abraham et al, 2014;Cherif et al, 2015;Rogers et al, 2014;Sidjabat et al, 2007). This is of great public health significance as those genes can be transmitted directly (via the food chain or environment) or indirectly (via mobilised plasmid) to other bacterial species…”

Section: Discussionmentioning

confidence: 99%

“…The bla CMY-2 gene is the most frequently detected AmpC β-lactamases in food-producing animals worldwide (Jacoby, 2009;Sidjabat et al, 2007). This gene has been detected among E. coli isolates sourced from animals as well as humans and may be transmitted via horizontal gene transfer via conjugation of plasmids such as IncI1 (Carattoli, 2009;Johnson et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

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Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

View full text Add to dashboard Cite

show abstract

“…All isolates considered significant were further identified by standard veterinary diagnostic techniques, including the Microbact 24E system (Medvet Diagnostics, Thebarton, South Australia, Australia), Streptex (Oxoid) for Streptococcus Lancefield grouping, and 16S rRNA gene sequencing (Sidjabat et al, 2007), as required. The immunohistochemistry for Toxoplasma gondii was performed by a commercial laboratory.…”

Section: Introductionmentioning

confidence: 99%

Postmortem Findings From Dugong (Dugong Dugon) Submissions to the University of Queensland: 1997–2010

Owen

Wilkie

2012

Journal of Wildlife Diseases

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ABSTRACT:To better record and characterize mortality in the declining population of dugong (Dugong dugon) in southeast Queensland, Australia, animals were collected and brought to the University of Queensland for postmortem examination. Fifty-five animals were examined over a 14-yr period. Human activities commonly caused the animal death. Several deaths were attributed to primary or secondary infections and idiopathic and degenerative diseases. A significant proportion of animals were found to have nonspecific signs of chronic debility, but the causes of disease and mortality in these cases remains to be identified.

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“…Resistance to extended-spectrum β-lactams in enterobacteria is mediated mainly by extended-spectrum β-lactamases (ESBLs) (Tamang et al, 2012;Ljungquist et al, 2016). ESBL-resistant enterobacteria are multidrug-resistant and exhibit resistance to all β-lactams and many classes of antibacterials including 2015a, Cozma et al, 2015b;Baede et al, 2015;Ljungquist et al, 2016), South America (Moreno et al, 2008;Rocha-Gracia et al, 2015;Oleivera et al, 2016;Carvalho et al, 2016), Asia (Sun et al, 2010;So et al, 2012;Tamang et al, 2014) and Australia (Sidjabat et al, 2007). No single report regarding the presence of ESBL-resistant enterobacteria in companion animals in Nigeria, exist in the literature whereas most Nigerian households keep dogs as pets, guard and/or hunting dogs.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Dogs

Anyanwu¹,

Ugwu²,

EZEKWELU³

et al. 2017

Not Sci Biol

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This study was conducted to isolate generic extended-spectrum β-lactam-resistant enterobacteria from household dogs in Nigeria, and to determine the antibacterial resistance profile of the isolates. Rectal swabs were collected from 100, randomly selected, apparently healthy household dogs. Isolation of ESBL-resistant enterobacteria was done using Mac Conkey agar supplemented with 4 µg/ml of ceftazidime. Phenotypic characterization of the isolates to generic level was done following standard biochemical methods. Phenotypic resistance of the isolates to antibacterial agents was determined using the disc diffusion method. Out of 27 ESBL-resistant enterobacterial isolates, 40.7% were Escherichia coli, 37% were Klebsiella species, 18.5% were Salmonella species, while 3.7% was Proteus species. Resistance of the E. coli isolates was 81.8% to ampicillin, 27.2% to streptomycin, 54.5% to ciprofloxacin and tetracycline, 45.4% to enrofloxacin, 90.9% to sulphamethoxazole/trimethoprim, 9.1% to amoxicillin/clavulanic acid and 0% resistant to gentamicin. Resistance of the Klebsiella isolates was 80% to ampicillin, 20% to streptomycin and amoxicillin/clavulanic acid, 30% to ciprofloxacin and enrofloxacin, 60% to tetracycline, 90% to sulphamethoxazole/trimethoprim and 10% to gentamicin. Resistance of the Salmonella isolates was 100% to sulphamethoxazole/trimethoprim, 80% to gentamicin and ampicillin, 60% to streptomycin and tetracycline, 20% to amoxicillin/clavulanic acid, and 0% to ciprofloxacin and enrofloxacin. The Proteus isolate was resistant to streptomycin and gentamycin. All the isolates were resistant to ceftazidime and cefotaxime. Resistance of the isolates to more than 3 classes of antibacterial agents tested was 81.8% for E. coli, 70% for Klebsiella and 100% for Salmonella, respectively. This study has shown that household dogs in Nigeria, are colonized by ESBL-resistant Enterobacteriaceae and are potential reservoirs and disseminators of these organisms.

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Identification of plasmid-mediated extended-spectrum and AmpC β-lactamases in Enterobacter spp. isolated from dogs

Cited by 42 publications

References 37 publications

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Postmortem Findings From Dugong (Dugong Dugon) Submissions to the University of Queensland: 1997–2010

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Dogs

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