Identification of Phytoplasmas Infecting Sour Cherry in Hungary

Varga, K.; Kölber, M.; Németh, M.; Ember, I.; Erdős, Z.; Biró, E.; Paltrinieri, Samanta; Martini, Marta; Bertaccini, Assunta

doi:10.17660/actahortic.2001.550.57

Cited by 8 publications

(5 citation statements)

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“…asteris'. Phytoplasmas from 16SrI-B subgroup were also reported in sour cherry in Lithuania (Valiūnas et al, 2007) and mahaleb in Hungary (Varga et al, 2001). The further study proved that sour cherry could be affected by phytoplasmas assigned to different subgroups within aster yellows group.…”

Section: Resultsmentioning

confidence: 84%

“…Ph. prunorum' was detected in sweet cherry in the Czech Republic and Poland (Fialová et al, 2004;Cieślińska, Morgaś, 2011) and in sour cherry in Hungary, the Czech Republic and Spain (Varga et al, 2001;Laviña et al, 2004;Ludvikova et al, 2011). Plants of Prunus species can be also infected by phytoplasmas trees with few leaf and rosetting symptoms including 16SrI, 16SrII, 16SrIII, 16SrV, 16SrVII, 16SrIX, 16SrX and 16SrXII.…”

Section: Introductionmentioning

confidence: 99%

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Multilocus sequence analysis of phytoplasmas detected in cherry trees in Poland

Cieślińska

Smolarek

2019

Zemdirbyste-Agriculture

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During 2010-2013 and 2016, shoot samples were collected from symptomatic and asymptomatic 85 sour cherry and 80 sweet cherry trees grown in 16 orchards located in major cherry cultivation areas in Central and Western Poland. Some sour cherry trees showed shoot proliferation, whereas dieback symptoms and leaf roll and yellowing were observed on some sweet cherry trees. The universal phytoplasma-specific primer pairs P1 and P7 derived from the ribosomal sequence within the 16S and 23S rRNA gene sequences and intergenic spacer region were used in direct polymerase chain reaction (PCR) followed by universal primers F1/B6 and primers R16(I)F1/R1 and R16(X)F1/R1 specific for 16SrI and 16SrX groups, respectively. Phytoplasmas were detected in two sweet cherry ('Trzebnica' and 'Kordia II.14') and one sour cherry ('cherry XVI.12') trees. Restriction fragment length polymorphism (RFLP) analyses conducted after digestion of F1/B6 products (~1.65 kb) with HhaI, RsaI, SspI and MseI enzymes indicated that sweet cherry trees were infected with 'Candidatus Phytoplasma prunorum' (16SrX-B). The restriction profiles for sour 'cherry XVI.12' sample were indistinguishable from those of the reference strain AY-1 of 'Candidatus Phytoplasma asteris' (16SrI-B). Multilocus sequence analysis of 16S DNA plus spacer region, secY and ribosomal protein (rp) operons confirmed the genetic diversity of phytoplasmas infecting cherry trees and showed the closest relationships 'Trzebnica' and 'Kordia II.14' isolates found in sweet cherry trees to the reference strains of 'Ca. Ph. prunorum' (16SrX-B). Depending on analysed sequenced region, the phytoplasma infecting sour 'cherry XVI.12' revealed close genetic relationship to phytoplasmas assigned to the different subgroups of aster yellows group (16SrI). SecY gene was the more informative marker for finer differentiation of the strains of phytoplasmas within 16SrI group and showed the highest genetic similarity of 'cherry XVI.12' isolate with 'Ca. Ph. asteris'-related strains.

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Section: Resultsmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Multilocus sequence analysis of phytoplasmas detected in cherry trees in Poland

Cieślińska

Smolarek

2019

Zemdirbyste-Agriculture

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“…P. asteris” (16SrI‐B) is associated with a number of symptoms in cherry trees: (a) premature drying of crown and production of heart‐shaped fruits of the cherry cv. Prime Giant in Italy (Paltrinieri et al., ); (b) small leaves, yellows, wilting and decline in Prunus mahaleb in Hungary (Varga et al., ); (c) sweet and sour cherry shoot proliferation and decline symptoms in Lithuania (Valiūnas, Jomantiene, & Davis, ; Valiūnas, Jomantiene, Ivanauskas, et al., ); (d) sweet cherry leaf roll and witches’ broom disease in Iran (Zirak, Bahar, & Ahoonmanesh, ); (e) branches proliferation, off‐season flowering and decline in sweet cherry in Turkey (Çaglayan et al., ); as well as (f) stunting, leaf rolling and yellowing of sweet and sour cherries in the Czech Republic (Navrátil et al., ). Phytoplasma strains associated with cherry virescence in China and with sour cherry shoot proliferation, yellowing and small leaves in Lithuania were classified as members of new subgroups in the aster yellows group, tentatively designated as 16SrI‐S and 16SrI‐Q, respectively (Gao et al., ; Valiūnas, Jomantiene, & Davis, , ; Valiūnas et al., ).…”

Section: Discussionmentioning

confidence: 99%

“Candidatus Phytoplasma asteris” and “Candidatus Phytoplasma mali” strains infecting sweet and sour cherry in the Czech Republic

Fráňová

Lenz

Přibylová

et al. 2017

Journal of Phytopathology

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A survey for phytoplasma diseases was conducted in a sweet and sour cherry germplasm collection and in cherry orchards within the Czech Republic during 2014–2015. Phytoplasmas were detected in 21 symptomatic trees. Multiple infections of cherry trees by diverse phytoplasmas of 16SrI and 16SrX groups and 16SrI‐A, 16SrI‐B, 16SrI‐L, 16SrX‐A subgroups were detected by restriction fragment length polymorphism (RFLP). Nevertheless, phylogenetic analysis placed subgroups 16SrI‐B and 16SrI‐L inseparable together onto one branch of phylogenetic tree. This is the first report of subgroups 16SrI‐A and 16SrI‐L in Prunus spp., and subgroup 16SrX‐A in sour cherry trees. Additionally, novel RFLP profiles for 16SrI‐A and 16SrI‐B‐related phytoplasmas were found in cherry samples. Phytoplasmas with these novel profiles belong, however, to their respective 16SrI‐A or 16SrI‐B phylogenetic clades.

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“…It was found that sour cherry with bunchy little leaf growths and leaf drop was infected by phytoplasma related to clover phyllody (formerly 16SrI-C) (Jomantienne et al 2011). Plum exhibiting symptoms of witches'-broom, shoot proliferation and abnormally small leaves, and sour cherry in Lithuania (Valiūnas et al 2007) as well as mahaleb in Hungary (Varga et al 2001) were infected by phytoplasma strains included into the 16SrI-B subgroup. Strains in the aster yellows group share ≥ 97% similarity in their 16S rDNA sequences (Lee et al 2004a).…”

Section: Aster Yellows Group (16sri 'Candidatus Phytoplasma Asteris')mentioning

confidence: 99%