Identification of phenotypic discriminating markers for intervertebral disc cells and articular chondrocytes

Clouet, Johann; Grimandi, G.; Pot-Vaucel, Marianne; Masson, Martial; Fellah, Hakim B; Guigand, Lydie; Chérel, Yan; Bord, E.; Rannou, François; Weiss, Pierre; Guicheux, Jérôme; Vinatier, Claire

doi:10.1093/rheumatology/kep262

Cited by 77 publications

(69 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Spontaneous disc regression is currently believed to be a consequence of macrophage activity. [35,36] type XI [37] all over, mostly NP regulates fibril diameter (smaller if this collagen is more abundant) influencing mechanical properties [35,38] beaded-filament forming collagens type VI all over, mostly NP helps cell fixation to the matrix and facilitates collagen bundles' sliding and lubrication [39,40] FACIT collagens type IX NP maintains matrix integrity [41,42] type XII AF might regulate fibrillogenesis [29,43] [66,[74][75][76]. However, when an imbalance occurs, degradation products might trigger inflammation.…”

Section: The Origin Of Inflammation In Intervertebral Discmentioning

confidence: 99%

Inflammation in intervertebral disc degeneration and regeneration

Molinos

Almeida

Caldeira

et al. 2015

J. R. Soc. Interface.

327

256

View full text Add to dashboard Cite

Intervertebral disc (IVD) degeneration is one of the major causes of low back pain, a problem with a heavy economic burden, which has been increasing in prevalence as populations age. Deeper knowledge of the complex spatial and temporal orchestration of cellular interactions and extracellular matrix remodelling is critical to improve current IVD therapies, which have so far proved unsatisfactory. Inflammation has been correlated with degenerative disc disease but its role in discogenic pain and hernia regression remains controversial. The inflammatory response may be involved in the onset of disease, but it is also crucial in maintaining tissue homeostasis. Furthermore, if properly balanced it may contribute to tissue repair/regeneration as has already been demonstrated in other tissues. In this review, we focus on how inflammation has been associated with IVD degeneration by describing observational and in vitro studies as well as in vivo animal models. Finally, we provide an overview of IVD regenerative therapies that target key inflammatory players.

show abstract

Section: The Origin Of Inflammation In Intervertebral Discmentioning

confidence: 99%

Inflammation in intervertebral disc degeneration and regeneration

Molinos

Almeida

Caldeira

et al. 2015

J. R. Soc. Interface.

327

256

View full text Add to dashboard Cite

show abstract

“…Therefore, we also examined phenotype of CD271 -MSCs and used PA-MSCs as a control cell population for the both isolated CD271 cell subsets. Although the phenotype of NP cells is closely associated with that of chondrocytes, still clear differences exist [41]. Similarly to the in vitro differentiation of MSCs into chondrocytes, their differentiation toward an NP-cell phenotype depends on diverse and specific parameters such as an appropriate choice of growth factors, 3D-matrix, intercellular contacts or environmental conditions mimicking the physiological status of IVD such as hypoxia [34].…”

Section: Discussionmentioning

confidence: 99%

The feasibility of the CD271+ and CD271– mesenchymal stromal cell enrichment toward nucleus pulposus-like cells

Jezierska-Woźniak

Barczewska

Habich

et al. 2017

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

introduction. Factors promoting nerve cell ingrowth are considered responsible for chronic back pain resulting from the intervertebral disc degeneration (IDD). One of the recent exploratory IDD treatments is stem cell transplantation therapy. The CD271 (low-affinity nerve growth factor receptor) has been identified as a marker of the most homogeneous mesenchymal stem cell (MSC) subset. It is capable of promoting differentiation along adipogenic, osteogenic and chondrogenic lineages and producing significantly higher levels of cytokines as compared to the total population of plastic adherence-mesenchymal stem cells (PA-MSCs). We investigated the ability of CD271 + MSCs to differentiate into chondrocyte-like cells of the nucleus pulposus (NP) of intervertebral disc. We also examined CD271 -MSCs, using PA-MSCs as a control cell population. Material and methods. Bone marrow derived PA-MSCs and its two subsets, CD271 -MSCs and CD271 + MSCs, were seeded in collagen scaffolds. After two weeks of growth in NP-differentiation medium, RNA was isolated from cells-scaffold constructs and was analyzed by q-PCR for expression of NP markers. Glycosaminoglycans were analyzed biochemically directly in cells-scaffold constructs. results. Expression of NP markers -extracellular matrix components such as aggrecan, collagen type II and glycosaminoglycans on both RNA and the protein levels -was significantly higher in CD271-MSCs compared to the CD271 + MSCs and PA-MSCs cell populations. Conclusions. CD271-MSCs may be superior candidates for NP restorative treatment compared to CD271 + MSCs and PA-MSCs due to their ability of expressing NP-supporting extracellular matrix components at levels higher than the other two studied MSC subsets.

show abstract

“…Although definitive phenotypic markers could not be identified, Clouet et al (2009) reported higher expression of type V collagen in rabbit AF than NP cells or articular chondrocytes. However, there are interspecies differences in gene expression (Pattappa et al, 2012).…”

Section: The Molecular Biology Of Anulus Fibrosus Components and Theimentioning

confidence: 97%

“…Although they are not absolutely definitive, they help us to understand the development and metabolism of NP cells and monitor the phenotype of MSC as they differentiate into an NP-like phenotype (Pattappa et al, 2012). In both healthy and degenerative discs, NP cells express chondrocyte markers such as SOX-9 and collagen type II (Clouet et al, 2009;Sive et al, 2002). In rats, COMP and MGP are more highly expressed in aged (24 months) rats than immature (three months) rats.…”

Section: Cellular Differentiationmentioning

confidence: 99%

Molecular Biology and Interactions in Intervertebral Disc Development, Homeostasis, and Degeneration, with Emphasis on Future Therapies: A Systematic Review

Aker¹,

Ghannam²,

Alzuabi³

et al. 2017

The Spine Scholar

View full text Add to dashboard Cite

The unique properties of the intervertebral disc (IVD) are evident in its structural complexity and functional importance for spinal support and stability. The contributions of the different cellular and extracellular components to the function of the IVD depend on their distinctive molecular features and pathways. Disruption of these molecular pathways influences the pathological changes involved in IVD degeneration. Therefore, the molecular features of the IVD have been the focus of interest for many researchers seeking to elucidate its normal functioning, potential pathologies, and appropriate therapies. The aim of the present article is to review the molecular aspects of IVD development, specific cellular markers, and the interactions between cellular and extracellular components responsible for homeostasis, degeneration and potential therapies. The literature available via PubMed and Google Scholar was reviewed and the relevant references in review articles were searched manually. Spine

show abstract

Identification of phenotypic discriminating markers for intervertebral disc cells and articular chondrocytes

Abstract: Our study shows that NP and AF cells exhibit a clearly distinguishable phenotype from that of AC. Type V collagen, MGP and HtrA1 could greatly help to discriminate among NP, AF and AC cells.

Cited by 77 publications

References 25 publications

Inflammation in intervertebral disc degeneration and regeneration

Inflammation in intervertebral disc degeneration and regeneration

The feasibility of the CD271+ and CD271– mesenchymal stromal cell enrichment toward nucleus pulposus-like cells

Molecular Biology and Interactions in Intervertebral Disc Development, Homeostasis, and Degeneration, with Emphasis on Future Therapies: A Systematic Review

Contact Info

Product

Resources

About