Identification of phenolic antioxidants and bioactives of pomegranate seeds following juice extraction using HPLC-DAD-ESI-MSn

Ambigaipalan, Priyatharini; Camargo, Adriano Costa de; Shahidi, Fereidoon

doi:10.1016/j.foodchem.2016.10.058

Cited by 95 publications

(76 citation statements)

References 38 publications

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“…The detected fragmentation pattern of tetrahydroxyflavone‐3 ‐ O ‐hexoside is shown in Figure b. Compounds 19 , 21 , 22, and 23 were proposed as rutin (Sun et al, ), quercetin‐3‐ O ‐hexoside, quercetin‐3‐ O ‐hexoside, and querctin ‐rhamanside (Ambigaipalan, Camargo, & Shahidi, ), respectively, by comparing with references. The existence and high relative intensity of MS/MS ion at m/z 300.02 suggested the linkage of glycoside on the 3‐OH (Vukics & Guttman, ).…”

Section: Resultsmentioning

confidence: 96%

“…Compound 1 ([M‐H] − , 181.0699) was regarded as veratric acid which has been reported in prunus amygdalus (Moqbel et al, ). Compound 4 ([M‐H] − 329.0837) was identified as vanillic acid hexoside due to the characteristic fragment ion at m/z 167.0341 (C 8 H 8 O 4 ) [vanillic acid‐H] − (Ambigaipalan et al, ). Compound 5 ([M‐H] − 315.0698) was identified as protocatechuic acid hexoside (isomer) by matching the fragment ions with those recorded in the reference (Sun et al, ).…”

Section: Resultsmentioning

confidence: 99%

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HPLC‐QTOF‐MS/MS profiling, antioxidant, andα‐glucosidase inhibitory activities ofPyracantha fortuneanafruit extracts

Wang

et al. 2019

J Food Biochem

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This study was carried out to optimize the solvent for extracting the antioxidants and α‐glucosidase inhibitors (AGIs) from Pyracantha fortuneana fruit (PFF) and the major chemical components were characterized by HPLC‐QTOF‐MS/MS. The results showed that 50% and 70% acetone (v/v, ml/ml) gave the best extraction efficiency on phenolics and total flavonoids, while 70% acetone and 50% methanol possess better recovery on protein and polysaccharides, respectively. In addition, the 50% and 70% acetone extracts gave the strongest radical scavenging ability and α‐glucosidase inhibitory activity (p > 0.05), but the Fe3+ reducing power of the 50% acetone extract was higher than that of 70% acetone. Correlation analysis indicated that phenolic acids and flavonoids were connected to the antioxidant activity and α‐glucosidase inhibitory activity closely. Moreover, 25 compounds including 7 flavonoids, 6 phenolic acids, 7 organic acids, 3 tannins, 1 terpene, and 1 alkaloid were identified or tentatively identified in the 50% acetone extract. Overall, 50% acetone can be a proper solvent for extracting antioxidants and AGIs from PFF. Practical applications Imbalance between production and clearance of reactive oxygen species (ROS) in the body could induce various chronic diseases. PFF is an edible fruit beneficial to human health; it is reported to be capable of optimizing blood glucose levels and may prevent premature aging. In the present study, PFF was found to be excellent in antioxidant activities and α‐glucosidase inhibitory ability; 50% acetone was found to be the best extraction solvent. In addition, the predominant phytochemical components of the 50% acetone extract were characterized. This study can promote further research of Pyracantha fortuneana in natural functional products, especially in the prevention of type II diabetes and its complication.

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Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

HPLC‐QTOF‐MS/MS profiling, antioxidant, andα‐glucosidase inhibitory activities ofPyracantha fortuneanafruit extracts

Wang

et al. 2019

J Food Biochem

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“…In this assay, a 0.02 mg/mL phenolic extract was used while the original concentration was 9.3 to 33.1 mg/mL. Ambigaipalan and others () reported that catechin (100 ppm) as a positive control exhibited 100% inhibition of LDL oxidation. In this study, camelina and sophia phenolic extract on LDL oxidation ranged from 14.86 ± 2.28% to 50.09 ± 2.80% (Figure B).…”

Section: Resultsmentioning

confidence: 99%

Biological Activities of Camelina and Sophia Seeds Phenolics: Inhibition of LDL Oxidation, DNA Damage, and Pancreatic Lipase and α‐Glucosidase Activities

Rahman

Ambigaipalan

Shahidi

2017

Journal of Food Science

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The seed phenolic extracts of camelina and sophia not only showed significant antioxidant activity but also effectively inhibited the activities of pancreatic lipase and α-glucosidase. Thus, camelina and sophia seed meals may serve as nutritional ingredients with bioactive phenolics that show in vitro antioxidant and biological effects. However, further research is required to confirm these health effects in vivo.

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“…Analytical methods were developed for the determination in different types of samples of phenolic compounds, such as chemiluminescence [12], spectrophotometry [13], capillary electrophoresis [14,15], and chromatography [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Phenolic Composition and Total Antioxidant Capacity by Electrochemical, Spectrophotometric and HPLC‐EC Evaluation in Portuguese Red and White Wines

et al. 2019

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Phenolic composition and in vitro total antioxidant capacity have been evaluated, in red and white Portuguese wines, by electrochemical methods, spectrophotometric methods, and reverse‐phase high performance liquid chromatography with electrochemical detection (HPLC‐EC). The different phenolic compounds present in seventeen red and white wines, with different grape varieties, and from different geographical locations, were successfully identified and detected. The wines in vitro total antioxidant capacity, using the method of capture of diphenilpicrilhydrazil (DPPH.) free radical “efficient concentration” (EC50), and the electrochemical quantitative index (EI), was determined. The wine with the highest total antioxidant capacity exhibited the lowest antioxidant power, and the EI and EC50 assays had a very good correlation.

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Identification of phenolic antioxidants and bioactives of pomegranate seeds following juice extraction using HPLC-DAD-ESI-MSn

Cited by 95 publications

References 38 publications

HPLC‐QTOF‐MS/MS profiling, antioxidant, andα‐glucosidase inhibitory activities ofPyracantha fortuneanafruit extracts

HPLC‐QTOF‐MS/MS profiling, antioxidant, andα‐glucosidase inhibitory activities ofPyracantha fortuneanafruit extracts

Biological Activities of Camelina and Sophia Seeds Phenolics: Inhibition of LDL Oxidation, DNA Damage, and Pancreatic Lipase and α‐Glucosidase Activities

Phenolic Composition and Total Antioxidant Capacity by Electrochemical, Spectrophotometric and HPLC‐EC Evaluation in Portuguese Red and White Wines

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