Identification of pea genotypes with enhanced thermotolerance using temperature induction response technique (TIR)

Srikanthbabu, Venkatachalayya; Ganeshkumar,; Krishnaprasad, B. T.; Ramaswamy, Gopalakrishna; Savitha, M.S.; Udayakumar, M.

doi:10.1078/0176-1617-00650

Cited by 74 publications

(60 citation statements)

References 35 publications

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“…Both total growth and per cent survival show a significant variation among different challenging temperatures. Based on above parameters, lethal temperature was standardized as 48 o C. This lethal temperature is the same as that of peas (Srikanthbabu et al, 2001) while in rice it was higher (52 o C) as recorded by Sapna (2011). Highest lethal temperature was recorded in groundnut (55 o C) by Gangappa et al (2006).…”

Section: Resultsmentioning

confidence: 99%

“…Thermotolerance can also be induced by gradual increase in temperature from sub-lethal to lethal temperature as would be experienced in natural environment (Larkindale et al, 2005). Plants growing in natural habitat at regular ambient temperature may experience high temperature that would be lethal in the absence of acclimation and hence, ability to acquire thermotolerance is of significant importance to plants (Srikanthbabu et al, 2001). …”

Section: Introductionmentioning

confidence: 99%

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Temperature induction response reveals intrinsic thermotolerant genotypes in soybean

Ange

Srividhya

Vijayalakshmi

et al. 2015

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The responses of soybean genotypes to high temperature for intrinsic tolerance was studied using Temperature Induction Response (TIR) technique in order identify the genotypes tolerant to high temperature stress. Seven soybean genotypes subjected to lethal and sub lethal temperatures showed significant variation for acquired thermotolerance. Thermotolerant genotypes ADT 1 and CoSoy1 identified by the TIR technique, demonstrated higher survival percentage, and lower growth reduction. Further the tolerant genotypes identified based on TIR also showed higher antioxidant enzymes activity implying the critical role of antioxidant in cellular thermotolerance. This clearly demonstrated that TIR can be effectively used for screening high temperature tolerance genotypes in soybean. This study also established the fact that the alternations in antioxidants during induction are vital for imparting tolerance to high temperature stress.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Temperature induction response reveals intrinsic thermotolerant genotypes in soybean

Ange

Srividhya

Vijayalakshmi

et al. 2015

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“…The best characterized aspect of acquired thermotolerance is production of heat shock proteins (HSPs) (Vierling 1991;Burke 2001;Iba 2002). Several other studies in different species demonstrated that upon acclimation in seedlings as well as plants, significant increase in HSPs (HSP 70, HSP 104, HSP 90 and HSP 18.1) occurred (Uma et al 1995;Kumar et al 1999;Srikanthbabu et al 2002;Senthil-Kumar et al 2003). The importance of acquired thermotolerence in crop plants under heat stress was reported by several authors (Uma et al 1995;Jayaprakash et al 1998;Kumar et al 1999;Burke et al 2000;Burke 2001;Srikanthbabu et al 2002;Senthil-Kumar et al 2003;2007).…”

Section: Introductionmentioning

confidence: 89%

Heat stress screening of peanut (Arachis hypogaea L.) seedlings for acquired thermotolerance

Selvaraj

Burow²,

Burke³

et al. 2011

Plant Growth Regul

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Heat can be one of the major abiotic stresses that adversely affect crop production worldwide at different stages of development. As field screening for heat tolerance can be inconsistent and seasonally-limited, it is important to develop a reliable protocol under controlled conditions that allows simultaneous screening of multiple genotypes. The objective of this research was to develop a straightforward laboratory protocol using acquired thermotolerance (ATT) in peanut seedlings as a measure of one mechanism of heat stress tolerance. Sixteen genotypes, including selected accessions of the US peanut minicore collection along with standard checks, were evaluated for acquired themotolerance in two independent experiments. A change in the temperature sensitivity of chlorophyll accumulation was used as an indicator of acquired thermotolerance. Pre-incubation at 38°C for 4 h before the 30-min 50°C challenge triggered the acquired thermotolerance system of the leaf disks, resulting in chlorophyll accumulation upon exposure to light. There was considerable variation among genotypes for ATT in both experiments. Genotypic ranking for mean ATT values were highly correlated (0.949) in both experiments. The effect of seed weight on ATT was not significant. This method is relatively simple and inexpensive and can be used to screen a large number of genotypes.

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“…The thermotolerant genotypes selected based on TIR technique at the seedling level are intrinsically tolerant at the plant level (Kumar et al, 1999;Senthil kumar et al, 2004;Srikanthbabu et al, 2002). In this method the early stages of the crop growth is subjected to gradual increase in the temperature and screening for survival at a challenging lethal temperature.…”

Section: Resultsmentioning

confidence: 99%

“…The induction temperature was standardized as 36 to 46°C at the rate of increase 2°C per hour.The reports suggest that genetic variability cannot be visualized when the plants are directly exposed to severe stress but only upon acclimation to prior severe stress (Fender and O' Cornell, 1990). The observed higher recovery growth of induced seedlings is mainly because of altered metabolism in response to acclimation as seen in sunflower (Kumar et al, 1999;Senthil Kumar et al, 2006), in sorghum (Howarthand skot, 1994), in beans (Keeler et al, 2000), in wheat (Burke, 1994(Burke, , 1998, and in groundnut (Srikanthbabu et al, 2002). …”

Section: Standardization Of Optimum Challenging and Induction Temperamentioning

confidence: 99%

Temperature induction response and accumulation of starch granules as indices to identify the thermotolerance of pulses at early growth stages

Partheeban

Vijayaraghavan

Sowmyapriya

et al. 2017

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In pulses, high temperature is a major limiting factor for yield decline. Tamil Nadu has a rich pulse germplasm with high genetic variability. Hence, the present study was designed to develop a technique to screen genotypes for high temperature tolerance. A rapid screening protocol was developed based on the principle of "acquired tolerance". Adapting this temperature induction response (TIR) technique to pulses, popular varieties of TNAU pulses of blackgram (VBG-07-001, VBG-08-003), redgram (CO-6, VBN-2) and soybean (CO-1, CO-2) were screened for thermotolerance. The challenging lethal temperature was standardized as 50°C for 3 hours at which 90 per cent of the seedling mortality occured. The induction temperature was standardized as 36°C to 40°C at which 48.3 per cent of growth reduction over control was noticed. Blackgram showed higher thermotolerance than other pulses in terms of survival percentage and seedling growth. Blackgram seeds showing contrasting behaviour to temperature stress tolerance namely VBG-07-001(tolerant) and VBG-08-003 (susceptible) were analysed for the accumulation and distribution of starch granules responsible for the grain filling under heat stress.

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Identification of pea genotypes with enhanced thermotolerance using temperature induction response technique (TIR)

Cited by 74 publications

References 35 publications

Temperature induction response reveals intrinsic thermotolerant genotypes in soybean

Temperature induction response reveals intrinsic thermotolerant genotypes in soybean

Heat stress screening of peanut (Arachis hypogaea L.) seedlings for acquired thermotolerance

Temperature induction response and accumulation of starch granules as indices to identify the thermotolerance of pulses at early growth stages

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