Identification of patterns of transmission of Salmonella within swine production systems using pulsed field gel electrophoresis (PFGE) and repetitive sequence polymerase chain reaction (REP-PCR): a quantitative analysis

Weigel, Ronald M.; Qiao, Baozhen; Barber, D. A.; Teferedegne, B.; Kocherginskaya, Svetlana A.; White, Bryan A.; Isaacson, Richard E.

doi:10.31274/safepork-180809-1189

Cited by 6 publications

(7 citation statements)

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“…However, the ERIC primers were the same and the protocol for DNA purification was similar, which does not justify the differences found in the ERIC-PCR results from the two works. Weigel et al (2001) also used rep-PCR with the same primers tested in the present study to type Salmonella and compared the profiles obtained with those generated by PFGE after DNA cleavage with three different enzymes. According to these authors, both methods were equivalent in detecting genetic variance and neither revealed improbable patterns of transmission.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and genotypic characterization of Salmonella Enteritidis isolates

Oliveira¹,

Bessa²,

Santos³

et al. 2007

Braz. J. Microbiol.

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In order to study the epidemiology of Salmonella Enteritidis outbreaks and determine the source of contamination so that a recurrence can be avoided, detailed characterization is necessary. Thus, the purpose of this study was to verify whether rep-PCR was able to discriminate among Salmonella Enteritidis isolates. Phage typing, detection of virulence genes and antimicrobial resistance testing were also associated to rep-PCR results. One hundred and two S. Enteritidis isolates from broiler carcasses, food, human, pigs, poultryrelated samples, and nine isolates from other countries were genotypically typed by REP-PCR, ERIC-PCR and BOX-PCR, collectively called rep-PCR. Phage typing, detection of virulence genes and antimicrobial resistance testing were also performed. Only three fingerprinting profiles were obtained with each rep-PCR method, with the majority of isolates belonging to the same profile. No relationship was observed between genotypic profile and year, place of isolation or source of infection. However, the less frequent rep-PCR profiles showed single antimicrobial resistance patterns. Although few strains isolated from swine were analyzed, different antimicrobial resistance patterns were observed. Furthermore, phage type 4 was not found in swine isolates. rep-PCR showed a lower discriminatory power as compared with antimicrobial resistance and phage typing, but the combination of genotypic and phenotypic methods was more discriminatory than any method alone, resulting in 48 different types.

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Section: Discussionmentioning

confidence: 99%

Phenotypic and genotypic characterization of Salmonella Enteritidis isolates

Oliveira¹,

Bessa²,

Santos³

et al. 2007

Braz. J. Microbiol.

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show abstract

“…The discriminatory power of these methods is evaluated and compared to PFGE in the present study. For ultimate Salmonella strain discrimination, an integrated approach using a highly discriminatory genotyping method together with phenotypic approaches is indispensable as shown previously [9,[14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology and diversity ofSalmonellaserovar Typhimurium in pigs using phenotypic and genotypic approaches

2005

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For epidemiological investigations of the most common and non-host-adapted Salmonella serotypes, such as Typhimurium, highly discriminatory approaches are essential. In the present study, we evaluated three genotyping methods; amplified fragment length polymorphism (AFLP), pulsed-field gel electrophoresis (PFGE) and repetitive palindromic extragenic-PCR (Rep-PCR) using 40 isolates. AFLP showed the highest discriminatory index (0.939), resolution and throughput. To determine clonality of Salmonella Typhimurium isolates and epidemiological relatedness in different commercial pig production units, we employed AFLP in combination with antimicrobial resistance pattern and phage typing. Salmonella serovar Typhimurium isolates (n=196) obtained from a longitudinal study of 18 pig farms over a 3-year period were studied. Using this approach, 16 distinct clonal types were identified. We found two common multidrug- resistant patterns including AmCmStSuTe and AmKmStSuTe. Two commonly multidrug- resistant phage types that are of known public health importance, DT104 and DT193, were also common. AFLP differentiated distinct clones within DT104, a phage type previously reported to be clonal. Fourteen of the clonal types were unique to one of the two production systems, showing diversity between independent commercial pig production systems located in the same geographical area. Clonal types obtained from nursery farms and corresponding finishing units were, however, similar.

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“…β-arrestin or ESBL gene was cloned and inserted into pET-28a plasmids using a transformation method described previously ( 31 ). Cells were checked by colony PCR and confirmed by sequence analysis of the PCR products as described previously ( 32 ).…”

Section: Methodsmentioning

confidence: 99%

Antibiotic resistance of Klebsiellaï¿½pneumoniae through β-arrestin recruitment-induced β-lactamase signaling pathway

et al. 2018

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Overuse and misuse of antibiotics leads to rapid evolution of antibiotic-resistant bacteria and antibiotic resistance genes. Klebsiella pneumoniae has become the most common pathogenic bacterium accountable for nosocomial infections due to its high virulence factor and general occurrence of resistance to most antibiotics. The β-lactamase signaling pathway has been suggested to be involved in antibiotic resistance against β-lactams in Klebsiella pneumoniae. In the present study, the molecular mechanism of the antibiotic resistance of Klebsiella pneumoniae was investigated and the results indicated involvement of the β-arrestin recruitment-induced β-lactamase signaling pathway. Antimicrobial susceptibility of Klebsiella pneumoniae was assessed using automated systems and extended-spectrum β-lactamase (ESBL) and β-arrestin expression levels in Klebsiella pneumoniae were analyzed by reverse-transcription quantitative PCR. β-lactam resistance in Klebsiella pneumoniae was determined using β-lactam agar screening plates. The results demonstrated that β-arrestin recruitment was increased in Klebsiella pneumoniae with antibiotic resistance (AR-K.P.) compared with that in the native Klebsiella pneumoniae strain (NB-K.P.). Increased production of ESBL was observed in AR-K.P. after treatment with the β-lactam penicillin. Of note, inhibition of β-arrestin recruitment significantly suppressed ESBL expression in AR-K.P. and in addition, genes encoding β-arrestin and ESBL were upregulated in Klebsiella pneumoniae. Restoration of endogenous β-arrestin markedly increased antibiotic resistance of Klebsiella pneumoniae to β-lactam. Knockdown of endogenous β-arrestin downregulated antibiotic resistance genes and promoted the inhibitory effects of β-lactam antibiotic treatment on Klebsiella pneumoniae growth. In conclusion, the present study identified that β-arrestin recruitment was associated with growth and resistance to β-lactams, which suggested that β-arrestin regulating ESBL expression may be a potential target for addressing antibiotic resistance to β-lactams in Klebsiella pneumoniae.

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Identification of patterns of transmission of Salmonella within swine production systems using pulsed field gel electrophoresis (PFGE) and repetitive sequence polymerase chain reaction (REP-PCR): a quantitative analysis

Cited by 6 publications

References 0 publications

Phenotypic and genotypic characterization of Salmonella Enteritidis isolates

Phenotypic and genotypic characterization of Salmonella Enteritidis isolates

Molecular epidemiology and diversity ofSalmonellaserovar Typhimurium in pigs using phenotypic and genotypic approaches

Antibiotic resistance of Klebsiellaï¿½pneumoniae through β-arrestin recruitment-induced β-lactamase signaling pathway

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