Identification of pathway-based toxicity in the BALB/c 3T3 cell model

Vaccari, Monica; Mascolo, Maria Grazia; Rotondo, Francesca; Morandi, Elena; Quercioli, Daniele; Perdichizzi, Stefania; Zanzi, Cristina; Serra, Stefania; Poluzzi, Vanes; Angelini, Paola; Grilli, Sandro; Colacci, Annamaria

doi:10.1016/j.tiv.2014.10.002

Cited by 20 publications

(15 citation statements)

References 37 publications

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“…In detail, about a quarter of the authors declared that the PAH content/the organic fraction is responsible for the observed toxic effects (e.g., [9,11,37,88]), and fewer authors correlate the toxic effects with the inorganic water-soluble fraction (e.g., [54,62]), while some authors concluded that both parts attribute to overall toxicity (e.g., [23,71]). In contrast, four authors could not correlate the PAH content to the observed effects [25,42,57,86]. Regarding the toxicity of the whole particles, Diociaiuti and colleagues concluded that the observed oxidative stress is mainly due to the interaction of the particle surface with the cell membrane [36].…”

Section: Studies' Conclusion On the Potential Of Combustion-derived Pmentioning

confidence: 97%

From the air to the water phase: implication for toxicity testing of combustion-derived particles

Schmidt

Altenburger

Kühnel

2017

Biomass Conv. Bioref.

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The biological effects of airborne particulate matter (PM) in humans stimulated many research activities in recent years. One type of particles contributing to PM are those derived from combustion processes. By using in vitro test systems for assessing the toxic effects of those particles, the transfer into liquid media is necessary. Thus the question arises, how the transfer of airborne particles into liquid suspensions should be realized and how the exposure of test organisms/cells to the particles can be done and monitored with regard to concentration and stability. Further, the characterization of the airborne particles regarding their composition seems to be of importance in order to understand the induced biological effects. Hence, this review gathers and evaluates results from in vitro and ecotoxicity studies, where the introduction of airborne particles into liquids is required. The aim was to learn on the current practice and recommend on the design of toxicity studies in the future. In total, about 80 papers were retrieved by literature search that deal with the assessment of biological effects of combustion-derived particles or their constituents. The papers were evaluated in detail with regard to the methodology applied. From this evaluation and based on expert knowledge regarding nanoparticle testing, recommendations for the assessment of PM applying toxicological assays were developed. To provide guidance, essential criteria are developed that should help in preparation, testing, and during the evaluation phase of toxicological assays. We further advise whole particle testing, complemented by testing of particle extracts and washed particles.

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Section: Studies' Conclusion On the Potential Of Combustion-derived Pmentioning

confidence: 97%

From the air to the water phase: implication for toxicity testing of combustion-derived particles

Schmidt

Altenburger

Kühnel

2017

Biomass Conv. Bioref.

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“…Oxidative DNA damage may be critical in carcinogenesis if it results in extensive enough changes to induce gene mutations. However, the induction of oxidative stress may also regulate gene expression either directly through activation of gene transcriptional pathways or indirectly through hypomethylation (Nishida and Kudo, 2013;Casey et al, 2015;Vaccari et al, 2015;Wu and Ni, 2015). Epigenetic mechanisms in relation to regulatory needs are reviewed by Greally and Jacobs (2013) and Marczylo et al (2016), and International Agency for Research on Cancer (IARC) cancer classification by Herceg et al (2013).…”

Section: What Are Non-genotoxic Carcinogens?mentioning

confidence: 99%

International regulatory needs for development ofan IATA for non-genotoxic carcinogenic chemical substances

Jacobs

Colacci

Louekari³

et al. 2016

ALTEX

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“…All CTA models provide morphological endpoints of onco-transformation, which can be used as phenotypic anchoring for mechanistic studies. An experimental protocol which combined the BALB/c 3T3 CTA and a global gene expression analysis was developed to highlight the cross-talk between genotoxic and non-genotoxic carcinogenic mechanisms in the pathway leading to malignant cell transformation ( Vaccari et al, 2015 ). Recent evidence suggests that the early steps in cancer initiation may be related to non-genotoxic carcinogenic mechanisms, which can drive genome instability and mutagenesis.…”

Section: Integrated Approaches For Testing Assessment (Iata)mentioning

confidence: 99%

Moving forward in carcinogenicity assessment: Report of an EURL ECVAM/ESTIV workshop

Corvi

Madia

Guyton

et al. 2017

Toxicology in Vitro

Self Cite

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There is an increased need to develop novel alternative approaches to the two-year rodent bioassay for the carcinogenicity assessment of substances where the rodent bioassay is still a basic requirement, as well as for those substances where animal use is banned or limited or where information gaps are identified within legislation. The current progress in this area was addressed in a EURL ECVAM- ESTIV workshop held in October 2016, in Juan les Pins. A number of initiatives were presented and discussed, including data-driven, technology-driven and pathway-driven approaches. Despite a seemingly diverse range of strategic developments, commonalities are emerging. For example, providing insight into carcinogenicity mechanisms is becoming an increasingly appreciated aspect of hazard assessment and is suggested to be the best strategy to drive new developments. Thus, now more than ever, there is a need to combine and focus efforts towards the integration of available information between sectors. Such cross-sectorial harmonisation will aid in building confidence in new approach methods leading to increased implementation and thus a decreased necessity for the two-year rodent bioassay.

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Identification of pathway-based toxicity in the BALB/c 3T3 cell model

Cited by 20 publications

References 37 publications

From the air to the water phase: implication for toxicity testing of combustion-derived particles

From the air to the water phase: implication for toxicity testing of combustion-derived particles

International regulatory needs for development ofan IATA for non-genotoxic carcinogenic chemical substances

Moving forward in carcinogenicity assessment: Report of an EURL ECVAM/ESTIV workshop

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