Identification of Pathogenic <i>Fusarium</i> spp. Responsible for Root Rot of <i>Angelica sinensis</i> and Characterization of Their Biological Enemies in Dingxi, China

Yang, Liuyan; Tian, Yuan; Zhao, Xia; Yue, Liang; Uwaremwe, Constantine; Zhou, Qin; Wang, Yun; Zhang, Yubao; Dun, Zhiheng; Cui, Zengtuan; Wang, Ruoyu

doi:10.1094/pdis-06-21-1249-re

Cited by 6 publications

(1 citation statement)

References 46 publications

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“…In the case of fungi, Fusarium and Penicillium were the most abundant genera, followed by Mortierella, which was mainly abundant in samples from the rhizosphere of asymptomatic trees. Different Fusarium species have been documented as saprophytes [66], opportunists [67], and phytopathogens, meaning they are capable of causing diseases in plants such as vascular wilting, root rot, and stem decay, which results in a negative impact on the health and yield of crops [68][69][70][71]. Moreover, Penicillium is a common necrotrophicsaprophytic genus that might play an important role in diseased roots since it has been able to exhibit a variety of lifestyles, including mutualism, commensalism, and parasitism [72].…”

Section: Discussionmentioning

confidence: 99%

Exploring Microbial Rhizosphere Communities in Asymptomatic and Symptomatic Apple Trees Using Amplicon Sequencing and Shotgun Metagenomics

Muñoz-Ramírez,

González-Escobedo,

Avila-Quezada

et al. 2024

Agronomy

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The rhizosphere is a dynamic and highly interactive habitat where diverse microbial communities are established, and it plays crucial roles in plant health and disease dynamics. In this study, microbial communities and functional profiles in the rhizosphere of both asymptomatic and symptomatic apple trees were investigated through amplicon sequencing and shotgun metagenomics. The research was conducted at a location in the municipality of Cuauhtemoc, Chihuahua State, Mexico, and a total of 22 samples were collected, comprising 12 for amplicon sequencing and 10 for shotgun metagenomic sequencing. Symptomatic trees were identified based on reddish branches and internal necrosis in the trunk and root, while asymptomatic trees exhibited a healthy physiology. The findings showed that the dominant bacterial phyla included Proteobacteria, Actinobacteria, and Bacteroidetes, with prevalent genera such as Streptomyces, Pseudomonas, and Rhodanobacter. The fungal communities featured Ascomycota, Mortierellomycota, and Basidiomycota, which were dominated by Fusarium, Penicillium, and Mortierella. In the fungal communities, Mortierellomycota, notably abundant in asymptomatic trees, holds potential as a biocontrol agent, as seen in other studies on the suppression of Fusarium wilt disease. The application of shotgun metagenomic sequencing revealed significant differences in alpha and beta diversities in bacterial communities, suggesting a health-dependent change in species composition and abundance. Functional profile analysis highlighted enzymatic activities associated with lipid synthesis/degradation, amino acid biosynthesis, carbohydrate metabolism, and nucleotide synthesis, which have been documented to participate in symbiotic relationships between plants. These insights not only contribute to understanding the dynamics of rhizosphere microbial activity but also provide valuable perspectives on the potential application of microbial communities for tree health and implications for the management of apple orchards.

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