Identification of pathogenic Aspergillus species by a PCR-restriction enzyme method

Mirhendi, Hossein; Diba, Kambiz; Kordbacheh, Parivash; Jalalizand, Nilufar; Makimura, K.

doi:10.1099/jmm.0.47319-0

Cited by 32 publications

(26 citation statements)

References 12 publications

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“…These results confirmed an affiliation of isolated probiotic strain to S. cerevisiae sp. Arlorio et al [1], using ITS1 and ITS4 primers, obtained amplification products of about 800 bp for S. cerevisiae baker's strain while Mirhendi et al [38], using the same primers for S. cerevisiae AJ544-253 strain, noticed PCR products at a level of about 850 bp.…”

Section: Yeast Identificationmentioning

confidence: 99%

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Chemical composition of the cell wall of probiotic and brewer’s yeast in response to cultivation medium with glycerol as a carbon source

Bzducha-Wróbel

Kieliszek

Błażejak

2013

Eur Food Res Technol

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The structure of cell wall of yeasts (genus Saccharomyces) is one of the factors that determine their health-promoting properties connected to the presence of b-glucans and mannoprotein. The aim of the study was to determine the influence of glycerol as a carbon source on structural polymers of cell wall (b-glucan and mannoprotein) of probiotic yeasts Saccharomyces cerevisiae var. boulardii and brewer's yeasts S. cerevisiae R9. Significant increase of the percentage of polysaccharide content in the cell wall dry weight of S. cerevisiae R9 brewer's yeasts was noted (in the range of 10-20 %) after cultivation in medium containing glycerol at a concentration of 2-5 % and pH 4.0. The highest content of carbohydrates in probiotic yeasts' cell wall (58 %) was observed after cultivation in medium containing 3 % of glycerol and pH 5.0. The cell wall of probiotic yeasts was characterized by higher content of mannoprotein comparing with cell wall preparation of brewer's yeasts S. cerevisiae R9 composed mainly of b-glucans. After cultivation in mediums with 2 and 3 % of glycerol, the cell of brewer's yeasts contained the highest amount of b(1,3/1,6)-glucan in dry weight of the cell wall (about 36 %). Glycerol at a concentration of 3 and 5 % also intensified mannoprotein biosynthesis in cell wall of S. cerevisiae R9, approximating their content to those noted in the cells of probiotic yeasts (about 29 % (w/w) of dry weight of the cell wall) after cultivation in a medium of pH 5.0 containing 3 % of glycerol.

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Section: Yeast Identificationmentioning

confidence: 99%

“…[1,10,38]. Application of ITS1 and ITS4 primers in DNA fragments amplification of S. cerevisiae yeasts causes an intensification of PCR product of about 840 bp.…”

Section: Yeast Identificationmentioning

confidence: 99%

Chemical composition of the cell wall of probiotic and brewer’s yeast in response to cultivation medium with glycerol as a carbon source

Bzducha-Wróbel

Kieliszek

Błażejak

2013

Eur Food Res Technol

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“…Over the last several years, molecular methods, including the rolling-cycle amplification, repetitive sequence-based PCR, PCR-restriction enzyme, reverse line blot assay, and DNA microarray methods, have been evaluated for Aspergillus species identification (7,14,19,22,25). Although these methods have been demonstrated to be useful for species identification, most of these methods (except the reverse line blot assay) are not amenable to multiplexing.…”

Section: Discussionmentioning

confidence: 99%

Development and Validation of a Microsphere-Based Luminex Assay for Rapid Identification of Clinically Relevant Aspergilli

2009

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A Luminex-based assay for the rapid identification of Aspergillus species was designed, optimized, and validated with 131 clinical isolates of Aspergillus fumigatus, A. flavus, A. niger, A. terreus, A. ustus, and A. versicolor. The six species-specific probes were directed toward the internal transcribed spacer 1 (ITS-1) region and tested in a multiplex format with results generated within 6 h. Species identifications generated by the Aspergillus Luminex assay were 100% concordant with results from comparative sequence analyses of the ITS-1 region and showed excellent specificity. The Aspergillus Luminex assay is a rapid, relatively simple method that may prove to be a useful diagnostic tool for rapid Aspergillus identification in clinical laboratory settings.

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“…The clearing around each colony is the cellulose degradation zone for the associated mutant confirm identification (Hansen et al 2008). PCR-RFLP has been used by researchers to differentiate between Aspergillus species (Merhendi et al 2007). Amplification using ITS1 and ITS4 conserved region primers gave an amplicon of 607 bp size which when digested with Hbl1 resulted in three fragments of 96, 179 and 332 bp corresponding to two restriction sites at 333 and 512 bp for Aspergillus terreus.…”

Section: Isolation and Identification Of The Cellulose Degrading Fungusmentioning

confidence: 99%

High solid saccharification using mild alkali-pretreated rice straw by hyper-cellulolytic fungal strain

Dixit

Shah

Madamwar

et al. 2015

Bioresour. Bioprocess.

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Background:The aim of this study was to use traditional mutagenesis to generate hyper-cellulolytic mutants with emphasis on stable, non-spore formers, shorter enzyme producing times and higher saccharification efficiency at high solid loadings. An in-house isolated strain of Aspergillus terreus (At) was identified, fingerprinted and mutated. A sequential process of mutation followed by stringent selection generated mutant At 9 , which produced optimal cellulase at day 4 instead of day 7, was non-spore former with high stability and grew on a lower pH than parental strain. At 9 cellulases were used successfully at high solid loads [up to 25 % (w/v)] in a modified system at 50 °C with reduced hydrolysis times compared to parent strain. Conclusion:In current work ultra violet (UV) mutagenesis and intelligent screening design combined with growth on a cheap substrate for enzyme production was demonstrated. With this work we present a single organism enzyme system with substantially lower production time and decreased saccharification time at high solid loads.

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Identification of pathogenic Aspergillus species by a PCR-restriction enzyme method

Cited by 32 publications

References 12 publications

Chemical composition of the cell wall of probiotic and brewer’s yeast in response to cultivation medium with glycerol as a carbon source

Chemical composition of the cell wall of probiotic and brewer’s yeast in response to cultivation medium with glycerol as a carbon source

Development and Validation of a Microsphere-Based Luminex Assay for Rapid Identification of Clinically Relevant Aspergilli

High solid saccharification using mild alkali-pretreated rice straw by hyper-cellulolytic fungal strain

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