Identification of PAN2 by Quantitative Proteomics as a Leucine-Rich Repeat–Receptor-Like Kinase Acting Upstream of PAN1 to Polarize Cell Division in Maize

Zhang, Xiaoguo; Facette, Michelle R; Humphries, John; Shen, Zhouxin; Park, Yeri; Sutimantanapi, Dena; Sylvester, Anne W.; Briggs, Steven P.; Smith, Laurie G.

doi:10.1105/tpc.112.104125

Cited by 90 publications

(135 citation statements)

References 44 publications

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“…Formation of a GMC (produced by an unknown mechanism) causes the flanking cells, called subsidiary mother cells (SMCs), to position their nuclei and divide toward the GMC, resulting in one SC adjacent to each GC. Two LRR receptor-like kinases, PANGLOSS1 (PAN1) and PAN2, are strong polarity markers that play an important role in orientating the asymmetric division of SMCs (Cartwright et al, 2009;Zhang et al, 2012).…”

Section: Extrinsic Cue-mediated Protein Pan1mentioning

confidence: 99%

Stomatal Development in Arabidopsis

Pillitteri

Dong

2013

The Arabidopsis Book

127

119

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Stomata consist of two guard cells that function as turgor-operated valves that regulate gas exchange in plants. In Arabidopsis, a dedicated cell lineage is initiated and undergoes a series of cell divisions and cell-state transitions to produce a stoma. A set of basic helix-loop-helix (bHLH) transcription factors regulates the transition and differentiation events through the lineage, while the placement of stomata relative to each other is controlled by intercellular signaling via peptide ligands, transmembrane receptors, and mitogen-activated protein kinase (MAPK) modules. Some genes involved in regulating stomatal differentiation or density are also involved in hormonal and environmental stress responses, which may provide a link between modulation of stomatal development or function in response to changes in the environment. Premitotic polarlylocalized proteins provide an added layer of regulation, which can be addressed more thoroughly with the identification of additional proteins in this pathway. Linking the networks that control stomatal development promises to bring advances to our understanding of signal transduction, cell polarity, and cell-fate specification in plants.

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Section: Extrinsic Cue-mediated Protein Pan1mentioning

confidence: 99%

Stomatal Development in Arabidopsis

Pillitteri

Dong

2013

The Arabidopsis Book

127

119

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“…Phenotypically similar, but unlinked, pan2 mutants (Zhang et al 2012) were also assessed to test the hypothesis that the pan genes influence the disease response through their known effect on stomatal morphology or related pathways. We tested pan1 and pan2 mutants for NLB and Stewart's wilt in replicated, multiyear trials and found a significant genotype effect (P-value ,0.0001) for both diseases.…”

Section: Association Mappingmentioning

confidence: 99%

Unraveling Genomic Complexity at a Quantitative Disease Resistance Locus in Maize

et al. 2014

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Multiple disease resistance has important implications for plant fitness, given the selection pressure that many pathogens exert directly on natural plant populations and indirectly via crop improvement programs. Evidence of a locus conditioning resistance to multiple pathogens was found in bin 1.06 of the maize genome with the allele from inbred line "Tx303" conditioning quantitative resistance to northern leaf blight (NLB) and qualitative resistance to Stewart's wilt. To dissect the genetic basis of resistance in this region and to refine candidate gene hypotheses, we mapped resistance to the two diseases. Both resistance phenotypes were localized to overlapping regions, with the Stewart's wilt interval refined to a 95.9-kb segment containing three genes and the NLB interval to a 3.60-Mb segment containing 117 genes. Regions of the introgression showed little to no recombination, suggesting structural differences between the inbred lines Tx303 and "B73," the parents of the fine-mapping population. We examined copy number variation across the region using next-generation sequencing data, and found large variation in read depth in Tx303 across the region relative to the reference genome of B73. In the fine-mapping region, association mapping for NLB implicated candidate genes, including a putative zinc finger and pan1. We tested mutant alleles and found that pan1 is a susceptibility gene for NLB and Stewart's wilt. Our data strongly suggest that structural variation plays an important role in resistance conditioned by this region, and pan1, a gene conditioning susceptibility for NLB, may underlie the QTL.T HE genes and loci that influence host-pathogen interactions vary in allele effects, specificities, and linkage relationships. While disease resistance can be conditioned by single genes with large effect (Bent 1996;Jones and Dangl 2006), the emerging model of resistance for many plant diseases is complex in nature, with many genes and loci functioning in concert and each contributing a small proportion of the total phenotypic variation Poland et al. 2011;Cook et al. 2012b). Each locus has a unique profile, with some loci contributing broadspectrum protection against diverse pathogen species and strains. Investigating these intricacies offers the opportunity to understand the diverse ways in which plants defend themselves against microbial assault.Correlated responses to multiple diseases have been observed in various germplasm panels, implying that there are loci and genes that condition broad-spectrum resistance (Rossi et al. 2006;Gurung et al. 2009;Wisser et al. 2011). At the chromosomal segment level, disease and insect resistance loci colocalize in a nonrandom fashion (McMullen and Simcox 1995;Williams 2003;Wisser et al. 2005) and loci have been identified that confer resistance to diverse pathogen isolates and taxa (Zwonitzer et al. 2010;Chung et al. 2011;Belcher et al. 2012). There is evidence to suggest that gene clusters can confer resistance to more than one disease. A cluster of germin-like ...

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“…This result indicates that unlike some developmental transcription factors, the TSH1 protein probably does not traffic from cell to cell (Whipple et al, 2010). In other examples, stably transformed maize lines expressing FP fusion proteins have also been used to study biosynthetic proteins (Christensen et al, 2013, Falcone Ferreyra et al, 2012 and proteins that regulate asymmetric cell divisions during leaf development (Humphries et al, 2011, Zhang et al, 2012. Transcriptional fusions have also been used to make hormone responsive reporters (Lee et al, 2009).…”

Section: Application Of Fluorescent Protein Marker Lines In Protein Lmentioning

confidence: 99%

Fluorescent protein marker lines in maize: generation and applications

Wu¹,

Luo²,

Zadrozny³

et al. 2013

Int. J. Dev. Biol.

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Fluorescent proteins (FP) have significantly impacted the way that we study plants in the past two decades. In the post-genomics era, these FP tools are in higher demand by plant scientists for studying the dynamics of protein localization, function, and interactions, and to translate sequence information to biological knowledge that can benefit humans. Although FP tools have been widely used in the model plant Arabidopsis, few FP resources have been developed for maize, one of the most important food crops worldwide, and an ideal species for genetic and developmental biology research. In an effort to provide the maize and cereals research communities with a comprehensive set of FP resources for different purposes of study, we generated more than 100 stable transformed maize FP marker lines, which mark most compartments in maize cells with different FPs. Additionally, we are generating driver and reporter lines, based on the principle of the pOp-LhG4 transactivation system, allowing specific expression or mis-expression of any gene of interest to precisely study protein functions. These marker lines can be used not only for static protein localization studies, but will be useful for studying protein dynamics and interactions using kinetic microscopy methods, such as fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectroscopy (FCS), and fluorescence resonance energy transfer (FRET). All of the constructs and maize marker lines are publicly available through our website, http://maize. jcvi.org/cellgenomics/index.php

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Identification of PAN2 by Quantitative Proteomics as a Leucine-Rich Repeat–Receptor-Like Kinase Acting Upstream of PAN1 to Polarize Cell Division in Maize

Cited by 90 publications

References 44 publications

Stomatal Development in Arabidopsis

Stomatal Development in Arabidopsis

Unraveling Genomic Complexity at a Quantitative Disease Resistance Locus in Maize

Fluorescent protein marker lines in maize: generation and applications

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