Identification of p74, a gene essential for virulence of baculovirus occlusion bodies

Kuzio, John; Jaques, R. P.; Faulkner, P.

doi:10.1016/0042-6822(89)90593-x

Cited by 142 publications

(113 citation statements)

References 17 publications

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“…In a previous study we reported that deletion of the C terminus of the AcMNPV protein designated p74 abolished infectivity by per os inoculation of insects but did not interfere with virus replication in cell culture (Kuzio et al, 1989). We report here studies showing that p74 is a structural component of the PDV phenotype, associated with the outside of the virion envelope, and is not detected in the budded form virion.…”

Section: Introductionmentioning

confidence: 51%

Analysis of p74, a PDV envelope protein of Autographa californica nucleopolyhedrovirus required for occlusion body infectivity in vivo.

Faulkner

Kuzio

Williams

et al. 1997

Journal of General Virology

141

122

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In nature, nuclear polyhedrosis viruses (NPV) are transmitted when susceptible insect larvae ingest viral occlusion bodies (OB). These dissociate in the alkaline environment of the midgut and release encapsulated virions (PDV) which bind to midgut epithelial cells and initiate an infection. A previous study showed that expression of the Autographa californica NPV (AcMNPV) p74 gene during replication is essential for the production of infectious OB. A set of p74 deletion and overexpression recombinants was used for the production and screening of monoclonal antibodies, and for an investigation of gross cytopathology and localization of p74. No differences in virus structure or morphogenesis were observed in infected cells when the p74 gene of AcMNPV was deleted, even though the infectivity of OB harvested from the cells was abolished when they were fed to Trichoplusia ni

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Section: Introductionmentioning

confidence: 51%

Analysis of p74, a PDV envelope protein of Autographa californica nucleopolyhedrovirus required for occlusion body infectivity in vivo.

Faulkner

Kuzio

Williams

et al. 1997

Journal of General Virology

141

122

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“…In addition, another protein, p87, appears to be present in both Autographa californica multiple nucleocapsid nuclear polyhedrosis virus (AcMNPV) and Orgyia pseudotsugata MNPV capsids (Miiller et aI., 1990;Lu & Carstens, 1991). In contrast, recent investigations by Kuzio et al (1989) suggest that a protein called p74 may be a specific component of PDV nucleocapsids that is required for infectivity in insects but not cultured cells.…”

mentioning

confidence: 89%

Immunocytochemical characterization of p24, a baculovirus capsid-associated protein

Wolgamot

Gross

Russell

et al. 1993

Journal of General Virology

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“…The ATG of the SpliMNPV rr1 ORF was in a favourable translational context (Kozak, 1983). SpliMNPV rr1 preceded an ORF with homology to the AcMNPV p74 gene (Kuzio et al, 1989). The SpliMNPV rr1 stop codon and the presumed SpliMNPV p74 start codon were separated by 101 nt.…”

Section: Location and Sequence Of The Rr1 Genes Of Semnpv And Splimnpvmentioning

confidence: 99%

Baculoviruses contain a gene for the large subunit of ribonucleotide reductase.

Strien

Faktor²,

Wang³

et al. 1997

Journal of General Virology

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In the genomes of two baculoviruses, Spodoptera exigua and S. littoralis multicapsid nucleopolyhedroviruses (SeMNPV and SpliMNPV, respectively), an open reading frame (ORF) encoding the large subunit of ribonucleotide reductase (RR1) was identified. The predicted amino acid sequences of SeMNPV and SpliMNPV RR1 showed high homology to RR1 proteins from eukaryotes (ca. 70 % and 80 % similarity, respectively). The amino acid residues thought to be involved in catalytic function were conserved in the baculoviral RR1 ORFs. The RR1 ORFs in SeMNPV and SpliMNPV were located in different genomic positions. In SeMNPV, the RR1 ORF was located upstream of the polyhedrin gene, in an anti-genomic orientation. In SpliMNPV, the RR1 ORF preceded the p74 gene. By searching databanks, sequences homologous to the N ter-

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Identification of p74, a gene essential for virulence of baculovirus occlusion bodies

Cited by 142 publications

References 17 publications

Analysis of p74, a PDV envelope protein of Autographa californica nucleopolyhedrovirus required for occlusion body infectivity in vivo.

Analysis of p74, a PDV envelope protein of Autographa californica nucleopolyhedrovirus required for occlusion body infectivity in vivo.

Immunocytochemical characterization of p24, a baculovirus capsid-associated protein

Baculoviruses contain a gene for the large subunit of ribonucleotide reductase.

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