Identification of Outer Membrane Proteins Altered in Response to UVC-Radiation in Vibrio parahaemolyticus and Vibrio alginolyticus

Abdallah, Fethi Ben; Lagha, Rihab; Ellafi, Ali; Namane, Abdelkader; Rousselle, Jean-Claude; Lenormand, Pascal; Kallel, Héla

doi:10.1007/s12088-012-0299-2

Cited by 21 publications

(11 citation statements)

References 31 publications

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“…Equally important, the outer membrane protein, ompC (gi 697053451) showed decreased expression after UV-B irradiation to the cultures of E. cloacae . This finding is consistent with the report of the UV-C mediated alterations in protein profile of marine food borne pathogens, Vibrio parahaemolyticus and V. alginolyticus ( Abdallah et al, 2012 ). It was reported that after UV-C irradiation several OMPs showed alterations including the appearance or disappearance of proteins and/or decrease in the expression level of certain OMPs.…”

Section: Discussionsupporting

confidence: 93%

UV-B Radiation Stress Causes Alterations in Whole Cell Protein Profile and Expression of Certain Genes in the Rice Phyllospheric Bacterium Enterobacter cloacae

et al. 2016

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Among the different types of UV radiation, UV-B radiation (280-315 nm) has gained much attention mainly due to its increasing incidence on the Earth’s surface leading to imbalances in natural ecosystems. This study deals with the effects of UV-B radiation on the proteome and gene expression in a rice phyllospheric bacterium, Enterobacter cloacae. Of the five bacteria isolated from rice leaves, E. cloacae showed the highest level of resistance to UV-B and total killing occurred after 8 h of continuous exposure to UV-B. Reactive oxygen species were induced by UV-B exposure and increased with increasing duration of exposure. Protein profiling by SDS-PAGE and 2-dimensional gel electrophoresis (2-DE) revealed major changes in the number as well as expression of proteins. Analysis of 2-DE gel spots indicated up/down-regulation of several proteins under the stress of UV-B radiation. Thirteen differentially expressed proteins including two hypothetical proteins were identified by MALDI-TOF MS and assigned to eight functional categories. Both the hypothetical proteins (gi 779821175 and gi 503938301) were over-expressed after UV-B irradiation; gi 503938301 was characterized as a member of FMN reductase superfamily whereas gi 779821175 seems to be a structural protein as it did not show any functional domain. That the expression of certain proteins under UV-B stress is indeed up-regulated was confirmed by qRT-PCR. Transcript analysis of selected gene including genes of hypothetical proteins (cp011650 and cp002886) showed over-expression under UV-B stress as compared to untreated control cultures. Although this study deals with a limited number of proteins, identification of differentially expressed proteins reported herein may prove useful in future studies especially for assessing their significance in the protection mechanism of bacteria against UV-B radiation stress.

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Section: Discussionsupporting

confidence: 93%

UV-B Radiation Stress Causes Alterations in Whole Cell Protein Profile and Expression of Certain Genes in the Rice Phyllospheric Bacterium Enterobacter cloacae

et al. 2016

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“…OmpU also plays a role in the Vibrio response to starvation. To counteract nutrient limitations in the environment, Vibrio produces stress proteins and activates pathways that enable long term survival (Abdallah et al ., ). After 8 months of starvation in seawater, a microarray comparing OMP profiles of V. parahaemolyticus and V. alginolyticus was performed (Abdallah et al ., ).…”

Section: Stress Relief Via Ompumentioning

confidence: 97%

“…To counteract nutrient limitations in the environment, Vibrio produces stress proteins and activates pathways that enable long term survival (Abdallah et al ., ). After 8 months of starvation in seawater, a microarray comparing OMP profiles of V. parahaemolyticus and V. alginolyticus was performed (Abdallah et al ., ). OmpU was highly expressed in V. parahaemolyticus, but not V. alginolyticus, suggesting that OmpU plays different roles in the starvation response in these two species.…”

Section: Stress Relief Via Ompumentioning

confidence: 97%

Vibrio responses to extracytoplasmic stress

DeAngelis

Saul-McBeth

Matson

2018

Environ Microbiol Rep

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Summary A critical factor for bacterial survival in any environment is the ability to sense and respond appropriately to any stresses encountered. This is especially important for bacteria that inhabit environments that are constantly changing, or for those that inhabit more than one biological niche. Vibrio species are unique in that they are aquatic organisms, and must adapt to ever‐changing temperatures, salinity levels and nutrient concentrations. In addition, many species of Vibrio colonize other organisms, and must also deal with components of the host immune response. Vibrio infections of humans and other organisms have become more common in recent years, due to increasing water temperatures in many parts of the world. Therefore, understanding how these ubiquitous marine bacteria adapt to their changing environments is of importance. In this review, we discuss some of the ways that Vibrios sense and respond to the variety of stresses that negatively affect the bacterial cell envelope. Specifically, we will focus on what is currently known about the σE response, the Cpx response and the contributions of OmpU to extracytoplasmic stress relief.

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“…ace, ctxAB, hlyA, ompU, ompW, rfbO1, rtxC, tcpA, toxR and zot genes ( Table 1). The ompW gene contributes significantly in the outer membrane protein profile of bacteria and it is species specific for V. cholerae [11,12]. Somatic O-antigen biosynthesis gene was positive for O1 type (rfbO1), providing molecular evidence for O1 serogroup.…”

Section: Distribution Of Virulent Genes Among the Isolatesmentioning

confidence: 99%

Molecular Characterization of Vibrio cholerae O1 Reveals Continuous Evolution of Its New Variants in India

et al. 2013

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Vibrio cholerae, the causing agent of cholera is still a major health challenge in most of the developing countries. In this study, V. cholerae strains collected from different cholera outbreaks in India over a period of past 7 years were found to have various toxigenic, pathogenic and regulatory genes viz. ctxAB, zot, tcp, hlyA, ace, ompU, ompW, rfbO1, toxT and toxR. The biotype specific genes rstR and rtxC revealed the El Tor biotype in majority of the isolates. However, variants among the isolates were found having genotype of both the biotypes. Sequencing of ctxB gene revealed the presence of altered ctxB of classical biotype with additional variations in isolates of 2007. Mismatch amplification mutation assay PCR also confirmed the isolates belonging to classical biotype. Antibiogram of the isolates revealed resistance for nalidixic acid, co-trimoxazole, streptomycin, and polymyxin B and susceptibility for tetracycline among most of the isolates from India. However, V. cholerae isolates from a recent outbreak in Eastern India were resistant to tetracycline. The study corroborated the continuous emergence and wide-spread of multidrug resistant El Tor variant strains in the Indian subcontinent.

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Identification of Outer Membrane Proteins Altered in Response to UVC-Radiation in Vibrio parahaemolyticus and Vibrio alginolyticus

Cited by 21 publications

References 31 publications

UV-B Radiation Stress Causes Alterations in Whole Cell Protein Profile and Expression of Certain Genes in the Rice Phyllospheric Bacterium Enterobacter cloacae

UV-B Radiation Stress Causes Alterations in Whole Cell Protein Profile and Expression of Certain Genes in the Rice Phyllospheric Bacterium Enterobacter cloacae

Vibrio responses to extracytoplasmic stress

Molecular Characterization of Vibrio cholerae O1 Reveals Continuous Evolution of Its New Variants in India

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