Identification of oropharyngeal squamous cell carcinomas with active HPV16 involvement by immunohistochemical analysis of the retinoblastoma protein pathway

Abstract

Viral oncogene RNA expression is regarded as reliable biomarker to identify oropharyngeal squamous cell carcinomas (OPSCC) with active HPV16 involvement. This study addressed whether the expression profile of the cellular proteins p16INK4a , pRb, Cyclin

Prevalence and Prognostic Significance of HPV in Laryngeal Squamous Cell Carcinoma in Northeast China

“…Holzinger et al found that 22% of HPV RNA+ OPSCC samples were p16−. In addition, among 14-21% of HPV− samples, the expression levels of p16 were upregulated with no evidence of an HPV-16 transforming infection [39]. In our study, we …”

Prognostic Biomarkers in Phase II Trial of Cetuximab-Containing Induction and Chemoradiation in Resectable HNSCC: Eastern Cooperative Oncology Group E2303

“…HPV-induced HNSCC are associated with low pRb. Therefore, low pRB may represent a surrogate biomarker for HPV association [36]. However, there was no association between p16 status and survival outcomes.…”

Combination of p16INK4a/Ki67 immunocytology and hpv polymerase chain reaction for the noninvasive analysis of HPV involvement in head and neck cancer

“…Comparing the 3 methods used in our study to prove the presence of HPV DNA (applied to patients [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20], it seems that HPV-DNA PCR on cytological suspension has higher sensitivity (8 positive cases) than HPV-DNA PCR using FFPE tissue specimens (7 positive cases) and HPV-DNA ISH (4 positive cases), respectively. However, the number of patients that we analyzed with all 3 methods was too small to finally evaluate the diagnostic sensitivity of these methods.…”

“…[12][13][14][15][16][17][18][19] Consequently, general anesthesia is necessary for tissue sampling in most cases. Brush biopsies of macroscopically suspect lesions, as performed in our study, are a practical, noninvasive, cheap, and relatively painless alternative Original Article method for the collection of cellular material from the oral cavity to perform further diagnostics (eg, the evaluation of cytomorphology, immunostaining, or PCR).…”

“…However, there is still no established standard method for HPV detection in HNSCC. Currently used methods include the detection of HPV DNA by polymerase chain reaction (PCR), 12 DNA in situ hybridization, 13 the Hybrid Capture 2 assay, 14 the Cervista HPV16/18 assay, 15 and the immunohistochemical detection of high-risk HPV infection associated protein biomarkers, for example, p16 INK4a , pRb, p53, and cyclin D1 [16][17][18][19] as well as the detection of E6 and E7 mRNA by real-time PCR. 12 In otorhinolaryngology, the influence of HPV infection on the tumor cell biology of HNSCC is quite a young field of research; in gynecology, the role of HPV infection of the uterine cervix has been intensively studied, 20 and the benefit of its diagnostic proof in cytological specimens is well documented.…”