Identification of organic compounds

Gasparič, J.; Svobodová, D.

doi:10.1016/s0021-9673(00)89866-0

Cited by 8 publications

(3 citation statements)

References 10 publications

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“…R M0 and b TLC values from equation (2) were considered as the best means of estimating the lipophilicity (R M0 ) [38] and the contact hydrophobic surface area (b TLC ) [39] of the tetrazolium salts.…”

Section: Methodsmentioning

confidence: 99%

Application of multivariate mathematical-statistical methods to compare reversed-phase thin-layer and liquid chromatographic behaviour of tetrazolium salts in Quantitative Structure-Retention Relationships (QSRR) studies

et al. 1998

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The widely used reversed-phase liquid chromatographic separation technique is especially useful to the separation of bioactive molecules [1].Although the support is generally silica with covalently bonded hydrocarbons [2] or various polymers to the polar surface [3], the alumina is used more frequently with coated polymers on the surface as well [4]. Not only polyethylene [5][6][7] but also various types of other polymers such as polyamine [8], polystyrene [9] and polypyrrole chloride [10] have been coated to silica or alumina basis.The polymer-coated alumina supports typically and successfully used the investigation or separation of basic solutes hardly eluted from silica-based supports such as various proteins [11] Abstract. The retention parameters of seven mono-and nine ditetrazolium salts were investigated by reversed-phase liquid chromatography (RP-LC) using polyethylene-coated alumina support with various concentration of eluent-mixtures and by reversedphase thin-layer chromatography (RP-TLC) using the same support and eluent-mixtures. Principal component analysis (PCA) followed by non-linear mapping (NLM) and varimax rotation was used for the determination of molecular substructures and physicochemical parameters accounting for the separation in the different methods and circumstances. Calculations proved that the steric and electronic parameters have the highest influence on the retention of tetrazolium salts. This investigation uncovered that the thin-layer chromatography is strictly independent from liquid chromatography. It has been established that multivariate mathematical-statistical methods are the most appropriate ways for evaluation of large data matrices in various chromatographic departures.

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Section: Methodsmentioning

confidence: 99%

Application of multivariate mathematical-statistical methods to compare reversed-phase thin-layer and liquid chromatographic behaviour of tetrazolium salts in Quantitative Structure-Retention Relationships (QSRR) studies

et al. 1998

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“…Chlorotrimethylsilane is volatile and reactive toward silicon hydroxyl groups, resulting in the replacement of the hydrophilic hydroxyl groups by hydrophobic silane. Compared to other modication procedures, such as physical cover with paraffin or silicone oil, 35 the vapour reaction of chlorotrimethylsilane with silica gel can provide a surface homogeneously modied in a thin layer, and without the coverage of the sample molecules that can lead to lower sensitivity during MS analysis. In addition, the silane does not inuence MS analysis as it is chemically immobilized on the stationary phase of TLC plates that cannot be removed during ESTASI.…”

Section: Surface Modication Of Normal Phase Silica Tlc Plates For Es...mentioning

confidence: 99%

Ambient in situ analysis and imaging of both hydrophilic and hydrophobic thin layer chromatography plates by electrostatic spray ionization mass spectrometry

2015

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Electrostatic spray ionization (ESTASI) mass spectrometry imaging (MSI), recently developed to profile spatial distribution of chemical and biochemical molecules on an insulating surface under ambient conditions, has been applied to identify and quantify chemicals from silica gel surfaces. Both hydrophilic or hydrophobic silica plates have been imaged with good resolution providing a direct identification of the samples. For the hydrophilic silica gel substrate, silanization of the plate before ESTASI-MS analysis was used to render the surface hydrophobic. ESTASI-MSI is shown to be a sensitive technique for the identification of a wide range of molecules of different polarities and chemical structures with minimal sample consumption in the femtomole range.

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“…106 for a review). If plates are prepared in the laboratory, the reagent is usually added to the stationary-phase slurry.…”

Section: B Partition Preadsorbent and Impregnated Layersmentioning

confidence: 99%

Basic TLC Techniques, Materials, and Apparatus

Sherma¹

2003

Handbook of Thin-Layer Chromatography

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Identification of organic compounds

Cited by 8 publications

References 10 publications

Application of multivariate mathematical-statistical methods to compare reversed-phase thin-layer and liquid chromatographic behaviour of tetrazolium salts in Quantitative Structure-Retention Relationships (QSRR) studies

Application of multivariate mathematical-statistical methods to compare reversed-phase thin-layer and liquid chromatographic behaviour of tetrazolium salts in Quantitative Structure-Retention Relationships (QSRR) studies

Ambient in situ analysis and imaging of both hydrophilic and hydrophobic thin layer chromatography plates by electrostatic spray ionization mass spectrometry

Basic TLC Techniques, Materials, and Apparatus

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