Identification of organ tissue types and skin from forensic samples by microRNA expression analysis

Sauer, Eva; Extra, Antje; Cachée, Philipp; Courts, Cornelius

doi:10.1016/j.fsigen.2017.02.002

Cited by 37 publications

(26 citation statements)

References 50 publications

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“…Previous studies reported the involvement of miR-28 and miR-153 in the Nrf2 regulatory network in different cell lines [50,51,52,53,54]. Moreover, miRNAs are known to be differentially expressed in cell- and tissue-specific manners in various species [32,33,34,35]. Taking these findings into consideration and based on in silico analysis, miR-153, miR-28 and miR-708 were investigated for their role in regulating Nrf2 under oxidative stress conditions in bovine granulosa cells.…”

Section: Discussionmentioning

confidence: 99%

“…MicroRNAs are short non-coding RNAs of 18–24 nucleotides long that play crucial role in posttranscriptional endogenous gene regulators, by binding to the 3′-untranslated region (3′-UTR) of the target mRNA resulting in either degradation of the target mRNA and\or reduced translation [30,31]. Species and tissue expression patterns of miRNAs have been reported previously [32,33,34,35]. Our previous studies showed the crucial role of miRNAs in several biological processes in bovine granulosa cells including proliferation, differentiation, stress response and apoptosis [36,37,38,39].…”

Section: Introductionmentioning

confidence: 99%

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Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function

Khadrawy

Gebremedhn

Salilew‐Wondim

et al. 2019

IJMS

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Nrf2 is a redox sensitive transcription factor regulating the expression of antioxidant genes as defense mechanism against various stressors. The aim of this study is to investigate the potential role of noncoding miRNAs as endogenous and quercetin as exogenous regulators of Nrf2 pathway in bovine granulosa cells. For this cultured granulosa cells were used for modulation of miRNAs (miR-28, 153 and miR-708) targeting the bovine Nrf2 and supplementation of quercentin to investigate the regulatory mechanisms of the Nrf2 antioxidant system. Moreover, cultured cells were treated with hydrogen peroxide to induce oxidative stress in those cells. Our results showed that, oxidative stress activated the expression of Nrf2 as a defense mechanism, while suppressing the expression of those miRNAs. Overexpression of those miRNAs resulted in downregulation of Nrf2 expression resulted in higher ROS accumulation, reduced mitochondrial activity and cellular proliferation. Quercetin supplementation showed its protective role against oxidative stress induced by H2O2 by inducing the expression of antioxidant enzymes. In conclusion, this study highlighted the involvement of miR-153, miR-28 and miR-708 in regulatory network of Nrf2 mediated antioxidant system in bovine granulosa cells function. Furthermore, quercetin at a low dose played a protective role in bovine granulosa cells against oxidative stress damage.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function

Khadrawy

Gebremedhn

Salilew‐Wondim

et al. 2019

IJMS

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“…As miRNAs have shown great promise for BFID, it is logical to infer that same potential to their use for organ tissue identification, in particular because of the miRNA's robustness and ability to withstand degradation. Sauer et al (2017) published the first report of the use of miRNA markers for the identification of brain, kidney, liver, lung, skin, heart muscle, and skeletal muscle. The researchers provided a comprehensive investigation by performing RT-qPCR of 15 preselected miRNAs on not just pristine samples, but also mixed, aged, and degraded samples.…”

Section: Organ Tissue Identificationmentioning

confidence: 99%

Potential applications of microRNA profiling to forensic investigations

Glynn

2019

RNA

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Within the forensic science community, there is a continued push to develop novel tools to aid in criminal investigations. microRNA (miRNA) analysis has been the focus of many researcher's attention in the biomedical field since its discovery in 1993; however, the forensic application of miRNA analysis has only been suggested within the last 10 years and has been gaining considerable traction recently. The primary focus of the forensic application of miRNA analysis has been on body fluid identification to provide confirmatory universal analysis of unknown biological stains obtained from crime scenes or evidence items. There are, however, other forensic applications of miRNA profiling that have shown potential, yet are largely understudied, and warrant further investigation such as organ tissue identification, donor age estimation, and more. This review paper aims to evaluate the current literature and future potential of miRNA analysis within the forensic science field.

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“…Other biomarkers, such as RNAs, have also been proposed and employed in forensic science [29][30][31] and, in addition, with advances in molecular biological techniques, novel approaches based on miRNA profi ling have been developed for a possible forensic application [32]. For example, the circulating miRNAs have been considered as potential tool for identifi cation of sex [33], body fl uids [34,35] or organ tissue types [36].…”

Section: Discussionmentioning

confidence: 99%

Long-term persistence of Saliva-derived microRNA in human dental calculus for Forensic investigations

Lippolis¹,

Cavallini²,

Panzarino³

et al. 2019

Forensic Sci Today

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Introduction:Human identifi cation is one of the major fi elds in forensic odontology especially when the dental remains are the only available evidence to individual identifi cation. In this context DNA analysis in teeth pulp remains the most accurate and reliable method for identifi cation, whereas RNA is less used for forensic purpose because it is generally considered a quickly degradable molecule. Recently some studies report that another excellent source of nucleic acids is the dental calculus or tartar.Method: Therefore to assess the RNA stability over time, we investigated 40 saliva-derived microRNAs (miRNAs), in archeological and modern tartar samples by classic PCR followed by quantitative PCR.Result: Three miRNAs were not present in both ancient and modern tartar samples, while 10 out of 37 miRNAs had signifi cant different levels in ancient than modern samples. Nine miRNAs were down-regulated and only one was up-regulated. Since these different levels were not induced by gum infl ammation and were independent to guanine-cytosine content in miRNA sequences, used as degradation biomarker, we hypothesized as a possible cause the different eating habits between ancient and modern populations. This hypothesis is also supported by recent studies that demonstrate an close relationship between nutrients and changes in miRNA expression in healthy individuals. Conclusion:Overall data show that miRNAs are more stable than other RNA types in ancient tartar and this could enlarge the possibility of their practical use for forensic purpose and bioarchaeological researches although to better understand the variability of some miRNA levels between ancient and modern populations requires further investigation.

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Identification of organ tissue types and skin from forensic samples by microRNA expression analysis

Cited by 37 publications

References 50 publications

Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function

Endogenous and Exogenous Modulation of Nrf2 Mediated Oxidative Stress Response in Bovine Granulosa Cells: Potential Implication for Ovarian Function

Potential applications of microRNA profiling to forensic investigations

Long-term persistence of Saliva-derived microRNA in human dental calculus for Forensic investigations

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