“…To detect various oncogene mutations ( AML1-ETO , CBFβ-MYH11 , PML-RARα , BCR-ABL , DEK-NUP214 , NUP98-NSD1 , MLL-AF4 ), cDNA was amplified using microarray real-time PCR after reverse transcription (RT) [ 23 ]. Each sample for PCR contained 1× PCR buffer, 2.5 mM of each dNTP, 10 pM forward and reverse primers, 6 pM FAM-labeled probe at the 5′-end, 10 ng of cDNA, and 5 units of Taq polymerase (Evrogen, Moscow, Russia).…”