Identification of octopus peptide and its promotion of β‐casein synthesis in a mouse mammary epithelial cell line

Abstract

Octopus protein hydrolysate has been reported to increase milk yield and milk protein production. In this paper, the utilization and underlying mechanisms of bioactive peptide fractions from octopus protein hydrolysate on β-casein expression in mouse mammary epithelial cells (HC11) were investigated. Fraction OPH3-1 significantly stimulated cell proliferation and β-casein synthesis in HC11 cells, which was purified by ultra-filtration and gel-filtration chromatography. The MWs of the peptides from OPH3-1 ranged from 525-2,578 Da and consisted of 7-26 amino acid residues. Most of the peptides demonstrated the typical characteristics of milk protein synthesis promotion, especially MGLAGPR, MGDVLNF, EAPLMHV, and TEAPLMHV. Additionally, the mRNA abundances of mTOR, S6K1, 4EBP1, JAK2, and STAT5 were significantly enhanced by OPH3-1, which was consistent with the increased β-casein expression. These results suggest that the OPH3-1 peptides can promote the proliferation of mammary epithelial cells and increase β-casein synthesis. Practical applications Breastfeeding mothers are generally recommended to take octopus soup as a daily diet to promote lactation. The peptides fraction OPH3-1 from the enzymatic hydrolysate of Octopus vulgaris which was revealed to significantly stimulate mammary epithelial cell proliferation and β-casein synthesis was obtained. This study suggests that octopus peptides can be used as nutritional supplements to increase the quantity and quality of milk production.

miR-204-5p and miR-211 Synergistically Downregulate the αS1-Casein Content and Contribute to the Lower Allergy of Goat Milk

“…STAT5 binds to the gene promoter region of milk proteins by recognizing its specific binding motifs . Prolactin and glucocorticoid activate the proximal promoter of CSN 2 and induce β-casein expression in mouse mammary epithelial cells. , Essential amino acid and prolactin increase CSN 2 expression and β-casein synthesis by activating STAT5 signaling in BMEC. , Furthermore, STAT5a binds with the CSN 2 promoter and activates β-casein transcription in immortalized GMEC . Consistent with these data, luciferase assays have shown that STAT5 also could activate CSN 2 promoter activity in GMEC.…”

“…42 Prolactin and glucocorticoid activate the proximal promoter of CSN2 and induce β-casein expression in mouse mammary epithelial cells. 43,44 Essential amino acid and prolactin increase CSN2 expression and β-casein synthesis by activating STAT5 signaling in BMEC. 45,46 Furthermore, STAT5a binds with the CSN2 promoter and activates β-casein transcription in immortalized GMEC.…”

Enhancing Milk Production by Nutrient Supplements: Strategies and Regulatory Pathways

“…Studies also showed that after stimulation of BMECs with tripeptides and lactogenic hormones, the abundance of PepT1 and PepT2 can be increased, thereby promoting milk fat synthesis [85,86]. In addition, it has been shown that OPH3-1, a fraction of the octopus protein hydrolysis product bioactive peptide, promotes mammary epithelial cell proliferation and casein synthesis in mice [87].…”