Identification of novel variants of interferon-tau gene in Bighorn sheep (Ovis canadensis mexicana), Pelibuey sheep (Ovis aries) and its expression in hybrid blastocysts (Ovis canadensis × Ovis aries)

Palma-IrizarryM.,; Damian-MatsumuraP.,; BetancourtM.,; RomoS.,; Ortiz, Guillermo Perales; Lagunas-MartínezA.,; Madrid-MarinaM., V.; MejíaO.,; DucolombY.,

doi:10.4141/cjas2011-023

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…39 Despite the differences in the length of the oestrous cycle and gestation, and the lower fertility of the domestic sheep inseminated with frozen semen of bighorn, it is possible to produce embryos, gestations and viable hybrids between these not very closely related species. 23,40 The duration of gestation can be modified by different factors, and the foetal genotype has a very significant effect on the gestation period, because within this genome, the paternal component is the most important. 41 In hybrids between donkey and horse (mules), the gestation period is closer to the father's species than to that of the mother.…”

Section: Experiments IImentioning

confidence: 99%

Progesterone promotes fetal growth in a restricted interspecies gestation (Ovis canadensis x Ovis aries)

Villanueva¹,

Cazadero²,

Mejía³

et al. 2018

Vet Méx OA

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Gestations between bighorn (Ovis canadensis) and domestic sheep (O. aries) can be considered for ex situ conservation of bighorn. In the first experiment, domestic sheep were inseminated with bighorn or domestic semen. Bighorn inseminated sheep showed lower fertility than domestic inseminated sheep (40% vs. 65%, p = 0.11). Bighorn inseminated sheep had longer gestation periods (152.13 days vs. 146.54 days, p < 0.001) and lower progesterone levels during the last third. Hybrid lambs weighed less than domestic lambs (2.46 kg vs. 5.10 kg, p < 0.001). Their placentas were not as long (48.67 cm vs. 72.17 cm, p < 0.001), were less wide (17.83 cm vs. 23.83 cm, p < 0.001), and the weight of cotyledons was lower (1.50 g vs. 3.20 g, p < 0.001). In the second experiment, hybrid embryos (O. canadensis × O. aries) were transferred into domestic recipients, and pregnant ewes were divided into the treated group, which had a progesterone daily dose of 25 mg from weeks 7 to 20, and the non treated group. Gestation in domestic sheep that received one hybrid embryo and progesterone reached 152.60 days, which was similar to the 153.33 days (p = 0.51) in the non treated sheep. Hybrid offspring of the group treated with progesterone were heavier, 3.41 kg, than the control, 2.21 kg (p < 0.001), and their placentas were longer (71.20 vs. 50.83 cm, p = 0.002). Although progesterone levels were lower in domestic females inseminated with bighorn and in the recipients of hybrid embryos, it is possible to establish pregnancies between both species and the birth of viable offspring. The administration of progesterone during gestation increases the length of the placenta and promotes higher birth weights of hybrids. promotes foetal growth in a restricted interspecies gestation (Ovis canadensis × Ovis aries). Veterinaria México OA. 2018;5(3).

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Section: Experiments IImentioning

confidence: 99%

Progesterone promotes fetal growth in a restricted interspecies gestation (Ovis canadensis x Ovis aries)

Villanueva¹,

Cazadero²,

Mejía³

et al. 2018

Vet Méx OA

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Developmental dynamics of cloned Mexican bighorn sheep embryos using morphological quality standards

Martínez

Pichardo

Avendaño

et al. 2020

Veterinary Medicine & Sci

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The developmental dynamics of cloned Mexican bighorn sheep ( Ovis canadensis mexicana ) embryos were evaluated based on morphological quality standards. Categories determined by standards were correlated with the embryonic development stage, number of nuclei and viability. The results showed no differences in the blastocyst rate between the experimental (cloned Mexican bighorn sheep embryos) and control (parthenogenetic domestic sheep embryos) groups ( p > .05), while type IV fragmentation was higher in clones ( p < .05). The standards allowed for the identification of embryos that divided at least once or fragmented after 24 hr of culture. The highest percentage of morulae appeared at 96 hr, the final stages of development: nonsegmented, blocked, fragmented and blastocysts appeared at 192 hr. Embryonic quality decreased over time, making 96 hr the ideal time point to predict the final morphological quality of embryos. Nuclear staining of the morulae and blastocysts showed that higher embryo quality was associated with a higher percentage of normal and viable blastomeres. The evaluated criteria allowed for descriptions of the dynamics, stage and quality of cloned Mexican bighorn sheep embryos with a high degree of reliability. In addition, developmental anomalies, including fragmentation, multinucleation and blocking, were identified.

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Identification of novel variants of interferon-tau gene in Bighorn sheep (Ovis canadensis mexicana), Pelibuey sheep (Ovis aries) and its expression in hybrid blastocysts (Ovis canadensis × Ovis aries)

Cited by 2 publications

References 27 publications

Progesterone promotes fetal growth in a restricted interspecies gestation (Ovis canadensis x Ovis aries)

Progesterone promotes fetal growth in a restricted interspecies gestation (Ovis canadensis x Ovis aries)

Developmental dynamics of cloned Mexican bighorn sheep embryos using morphological quality standards

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