Identification of novel vaccine candidates against cryptosporidiosis of neonatal bovines by reverse vaccinology

Tomazic, Mariela Luján; Rodríguez, Anabel Elisa; Lombardelli, Joaquín Andrés; Poklépovich, Tomás; Garro, Carlos; Galarza, R.; Tiranti, Karina; Florín-Christensen, Mónica; Schnittger, Leonhard

doi:10.1016/j.vetpar.2018.11.007

Cited by 9 publications

(8 citation statements)

References 22 publications

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“…In studies, proteins and glycoproteins anchored to the parasite's surface have been identified via glycosylphosphatidylinositol (GPI), responsible for the motility, fixation and invasion of host cells, which are considered targets for generating an appropriate antibody response (Askari et al, 2016;Tomazic et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…However, the absence of a means of propagating this protozoan in vitro makes it difficult to identify these surface antigens. One solution found was the use of reverse vaccinology and through bioinformatics tools it was possible to identify the candidate antigens for the development of a vaccine (Tomazic et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Cryptosporidium parvum infections generate significant economic losses, due to severe diarrhea, impaired development and even death of newborn calves. These can be reservoirs of this protozoan, which characterizes them as an important source of zoonotic transmission (Lippuner et al, 2018;Tomazic et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…The study of the structural and functional characteristics of the candidate antigens for the synthesis of an effective immunogen is essential (Yang et al, 2016), since commercial vaccines are not available for animal or human protection or as mechanisms to prevent environmental contamination (Tomazic et al, 2018;Tosini et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Vaccine development for cryptosporidiosis: Systematic review

Silva

Oliveira

Marta

et al. 2021

RSD

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Cryptosporidium spp. are opportunistic parasites with zoonotic potential transmitted by ingestion of contaminated water and food, the infection consists of severe diarrhea leading to the death of babies and immunocompromised individuals. In the veterinary, it can generate economic losses due to the death of calves, these animals being a possible source of transmission of the parasite. Nitazoxanide and halofuginone are the only drugs approved for treatment in humans and calves respectively, however there are restrictions on their use. There is still no vaccine against cryptosporidiosis in humans or animals and its development is a great challenge. Our objective with the present work was to write a systematic review of the literature addressing the progress of studies on vaccines against cryptosporidiosis. The recommended guidelines for RSL were used, with the aid of the StArt software (State of the Art through Systematic Rewiews). The research took place in the databases: Lilacs, PubMed, Scielo, Science Direct, Scopus, Embase and Medline. In 30% of the selected articles, the calves were the study animal and in 50% the mice; 40% of the studies were with vaccines derived from recombinant proteins and 30% of the research was aimed at inhibiting the parasite from entering cells and activating the host's protective immunity. So far, a vaccine with no proven efficacy or an ideal cost-benefit has been developed. Many candidate antigenic targets for a vaccine have been characterized, in addition to elucidating the immunogenicity mechanism of Cryptosporidium spp. in the infected individual. However, another antigenic targets for a vaccine can be searched in future studies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Vaccine development for cryptosporidiosis: Systematic review

Silva

Oliveira

Marta

et al. 2021

RSD

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“…As in vitro culturing will likely be restricted to some laboratories, a convenient approach for the identification of additional vaccine candidates without parasite cultivation is reverse vaccinology. Indeed, by focusing onto the GPI-anchored proteome, this approach has allowed identification of a number of hitherto unknown vaccine candidates for subsequent use in vaccination trials [181,182]. Interestingly, an exclusive immunoinformatics approach to design a multisubunit vaccine against C. parvum has been recently reported [183].…”

Section: Vaccines Against Bovine Cryptosporidiosismentioning

confidence: 99%

Pursuing effective vaccines against cattle diseases caused by apicomplexan protozoa

Florín-Christensen¹

2021

CABI Reviews

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Apicomplexan parasites are responsible for important livestock diseases that affect the production of much needed protein resources, and those transmissible to humans pose a public health risk. Vaccines, recognized as a cost-effective and environmentally friendly method for the prevention of infectious diseases in livestock, can avert losses in food production and decrease the exposure of humans to zoonotic pathogens. This review focuses on the need for and advances in vaccine development against the apicomplexan parasites Theileria spp., Babesia spp., Toxoplasma gondii, Neospora caninum, Eimeria spp., Besnoitia spp., Sarcocystis spp., and Cryptosporidium parvum. Together, the effect of these parasites on the cattle industry worldwide causes an enormous burden, yet they remain poorly controlled and very few effective and practical vaccines against them are available. Vaccine development is hampered by our scarce and limited knowledge of the biology and mechanisms of pathogenesis of these microorganisms, and the absence of correlates of host immune protection. More studies focused on these aspects as well as on the identification of parasite vulnerabilities that can be exploited for vaccine design are needed. Novel "omics" and gene editing approaches in understanding complex parasite biology together with advances in vaccinology will facilitate the development of effective, sustainable, and practical vaccines against cattle diseases caused by apicomplexan parasites. Such vaccines will help prevent animal and human diseases and allow production of enough animal protein to feed the growing human population in the twenty-first century and beyond.

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