Identification of novel recombinants of hepatitis B virus genotypes F and G in human immunodeficiency virus-positive patients from Argentina and Brazil

Araújo, Natalia M.; Araujo, Oscar C.; Silva, Edinete M.; Villela-Nogueira, Cristiane Alves; Nabuco, Leticia Cancella; Paraná, Raymundo; Bessone, Fernando; Gomes, Saint Clair; Trépo, Christian; Kay, Alan

doi:10.1099/vir.0.047324-0

Cited by 23 publications

(16 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, the Brazilian Amazon region is a highly endemic area for HBV and hepatitis delta virus (HDV); in this region, HBV/F has a higher frequency among HBV-HDV co-infected patients than in the general HBV-monoinfected population [35,36]. Previously, only two Brazilian HBV/F complete genome sequences, GenBank accession numbers X69798 [37] and HE981181 [38] were available, limiting the contribution of Brazilian isolates to the phylogenetic studies of HBV/F.…”

Section: Introductionmentioning

confidence: 99%

Phylogeography and evolutionary history of hepatitis B virus genotype F in Brazil

Mello

Araujo

Lago

et al. 2013

Virol J

View full text Add to dashboard Cite

BackgroundHepatitis B virus (HBV) genotype F (HBV/F) is considered to be indigenous to the Americas, but its emergence and spread in the continent remain unknown. Previously, only two HBV/F complete genome sequences from Brazil were available, limiting the contribution of Brazilian isolates to the phylogenetic studies of HBV/F. The present study was carried out to assess the proportion and geographic distributions of HBV/F subgenotypes in Brazil, to determine the full-length genomic sequences of HBV/F isolates from different Brazilian geographic regions, and to investigate the detailed evolutionary history and phylogeography of HBV/F in Brazil.MethodsComplete HBV/F genomes isolated from 12 Brazilian patients, representing the HBV/F subgenotypes circulating in Brazil, were sequenced and analyzed together with sequences retrieved from GenBank, using the Bayesian coalescent and phylogeographic framework.ResultsPhylogenetic analysis using all Brazilian HBV/F S-gene sequences available in GenBank showed that HBV/F2a is found at higher frequencies countrywide and corresponds to all sequences isolated in the Brazilian Amazon Basin. In addition, the evolutionary analysis using complete genome sequences estimated an older median ancestral age for the Brazilian HBV/F2a compared to the Brazilian HBV/F1b and HBV/F4 subgenotypes, suggesting that HBV/F2a represents the original native HBV of Brazil. The phylogeographic patterns suggested a north-to-south flow of HBV/F2a from Venezuela to Brazil, whereas HBV/F1b and HBV/F4 strains appeared to have spread from Argentina to Brazil.ConclusionsThis study suggests a plausible route of introduction of HBV/F subgenotypes in Brazil and demonstrates the usefulness of recently developed computational tools for investigating the evolutionary history of HBV.

show abstract

Section: Introductionmentioning

confidence: 99%

Phylogeography and evolutionary history of hepatitis B virus genotype F in Brazil

Mello

Araujo

Lago

et al. 2013

Virol J

View full text Add to dashboard Cite

show abstract

“…Altogether, these characteristics hamper virus replication and make HBV-G mono-infection a rarely observed event [14-16]. Apparently, HBV-G replication can be rescued by co-infection with another HBV genotype, and such dual infections have been observed repeatedly with HBV genotype A in Japan [17,18], the USA [19,20], Spain [21] and Canada [22], with HBV genotypes A, C or D in Germany [23,24], with HBV genotype H in Mexico [25], and with HBV genotype F in Argentina [26]. In fact, rescue by another genotype can be very effective, even to the point where HBV-G outcompetes the co-infecting strain [9,17,19].…”

Section: Introductionmentioning

confidence: 99%

High prevalence of hepatitis B virus dual infection with genotypes A and G in HIV-1 infected men in Amsterdam, the Netherlands, during 2000-2011

Kuyl

Zorgdrager

Hogema³

et al. 2013

BMC Infect Dis

View full text Add to dashboard Cite

BackgroundHepatitis B virus (HBV) is divided into 8 definite (A-H) and 2 putative (I, J) genotypes that show a geographical distribution. HBV genotype G, however, is an aberrant genotype of unknown origin that demonstrates severe replication deficiencies and very little genetic variation. It is often found in co-infections with another HBV genotype and infection has been associated with certain risk groups such as intravenous drug users and men having sex with men (MSM). We aimed to estimate the prevalence of HBV-G in the Netherlands by analysing samples from HBV-positive patients visiting the Academic Medical Center in Amsterdam.MethodsNinety-six HBV-infected patients, genotyped as HBV-A or HBV-G infected, were retrieved from the clinical database. Blood plasma samples were analysed with a newly-developed real-time PCR assay that detects HBV-A and HBV-G. For three patients, the HBV plasma viral load (pVL) of both genotypes was followed longitudinally. In addition, three complete genomes of HBV-G were sequenced to determine their relationship to global HBV-G strains.ResultsTen HBV-G infections were found in the selected Dutch patients. All concerned HIV-1 infected males with HBV-A co-infection. Dutch HBV-G strains were phylogenetically closely related to reference HBV-G strains.ConclusionsIn this study, HBV-G infection in the Netherlands is found exclusively in HIV-1 infected men as co-infection with HBV-A. A considerable percentage (37%) of men infected with HBV and HIV-1 are actually co- infected with two HBV genotypes.

show abstract

“…This means that the third position of the polymerase codons correspond to the second position of the S codons. Nucleotide substitutions at the polymerase second codon position affects the amino acid in both RT and HBsAg, and this position has the highest degree of conservation in its nucleotide and amino acid sequence, like the RNAseH region of the polymerase or the PreS1 region [68,69]. This could explain why we did not find recombination events within this conserved region.…”

Section: Discussionmentioning

confidence: 72%

“…HBV recombinants in Latin America have been reported previously [31,67]. An F3/A1 recombinant was found in an Afro-Colombian population [68], and an F4/D2 recombinant was reported in Argentina [69]. ''Hot spots'' for HBV genome recombination events have been described within the core region, pre-S1, pre-S2/S, polymerase, and X gene [69].…”

Section: Discussionmentioning

confidence: 95%

Genetic variability of hepatitis B virus in Uruguay: D/F, A/F genotype recombinants

López¹,

Flichman

Mojsiejczuk

et al. 2015

Arch Virol

View full text Add to dashboard Cite

Hepatitis B virus (HBV) infection is a serious global health problem. Approximately 2 billion people worldwide have been infected, and approximately 350 million individuals currently suffer from HBV-induced chronic liver infection, which causes 600,000 deaths annually from chronic hepatitis, cirrhosis and hepatocellular carcinoma. HBV is classified in eight genotypes (A-H), and two more have been proposed (I-J). In this paper, complete genome sequences of nine Uruguayan HBV are reported. Five samples belong to genotype F1b and one to genotype A2. Three HBV recombinants were detected: A1/F1b, A2/F1b and D3/F1b. The following mutations were detected: a G1896A substitution, a 33-nucleotide deletion from position 2896 to 2928 in the Pre-S1 region involving Pre-S1 residues 3-13, a 33-nt deletion in the Pre-S1 region involving nt 2913-2945 and Pre-S1 residues 9-19. More F genotypes strains than expected were detected in this study, supporting the hypothesis that there are more people of indigenous origin than declared in our population. Also, one third of the samples analyzed were recombinants. This cannot be explained by the low HBV prevalence in Uruguay, but a high HBV infection rate in drug addicts and dialysis patients could act in favor of multiple-genotype HBV infections that could lead to recombination.

show abstract

Identification of novel recombinants of hepatitis B virus genotypes F and G in human immunodeficiency virus-positive patients from Argentina and Brazil

Cited by 23 publications

References 58 publications

Phylogeography and evolutionary history of hepatitis B virus genotype F in Brazil

Phylogeography and evolutionary history of hepatitis B virus genotype F in Brazil

High prevalence of hepatitis B virus dual infection with genotypes A and G in HIV-1 infected men in Amsterdam, the Netherlands, during 2000-2011

Genetic variability of hepatitis B virus in Uruguay: D/F, A/F genotype recombinants

Contact Info

Product

Resources

About