Identification of Novel Plasmids Containing the Tigecycline Resistance Gene <i>tet</i>(X4) in <i>Escherichia coli</i> Isolated from Retail Chicken Meat

Lv, Hong; Huang, Weifeng; Lei, Gaopeng; Liu, Li; Zhang, Lin; Yang, Xiaorong

doi:10.1089/fpd.2020.2822

Cited by 5 publications

(2 citation statements)

References 10 publications

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“…In addition, five tet (X4)-positive E. coli strains were isolated from retail chicken during routine monitoring of ARGs in the Sichuan market in 2020. Interestingly, one of the tet (X4)-carrying plasmids from retail chicken was 99% identity to the pig-derived tet (X4)-carrying plasmid, and others had the tet (X4) gene localized on hybrid plasmids ( Lv H. et al, 2020 ). This phenomenon suggests that tet (X4)-carrying plasmids can spread among different animals, which lead to the dissemination of tet (X4) in the ecological environment.…”

Section: Prevalence Of Tet (X4)mentioning

confidence: 99%

Dissemination and prevalence of plasmid-mediated high-level tigecycline resistance gene tet (X4)

Zhang

Wen²,

Wang³

et al. 2022

Front. Microbiol.

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With the large-scale use of antibiotics, antibiotic resistant bacteria (ARB) continue to rise, and antibiotic resistance genes (ARGs) are regarded as emerging environmental pollutants. The new tetracycline-class antibiotic, tigecycline is the last resort for treating multidrug-resistant (MDR) bacteria. Plasmid-mediated horizontal transfer enables the sharing of genetic information among different bacteria. The tigecycline resistance gene tet(X) threatens the efficacy of tigecycline, and the adjacent ISCR2 or IS26 are often detected upstream and downstream of the tet(X) gene, which may play a crucial driving role in the transmission of the tet(X) gene. Since the first discovery of the plasmid-mediated high-level tigecycline resistance gene tet(X4) in China in 2019, the tet(X) genes, especially tet(X4), have been reported within various reservoirs worldwide, such as ducks, geese, migratory birds, chickens, pigs, cattle, aquatic animals, agricultural field, meat, and humans. Further, our current researches also mentioned viruses as novel environmental reservoirs of antibiotic resistance, which will probably become a focus of studying the transmission of ARGs. Overall, this article mainly aims to discuss the current status of plasmid-mediated transmission of different tet(X) genes, in particular tet(X4), as environmental pollutants, which will risk to public health for the “One Health” concept.

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Section: Prevalence Of Tet (X4)mentioning

confidence: 99%

Dissemination and prevalence of plasmid-mediated high-level tigecycline resistance gene tet (X4)

Zhang

Wen²,

Wang³

et al. 2022

Front. Microbiol.

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“…Unlike pWWTP-01 and pWWTP-02, there were no heavy-metal-, disinfectant-, or virulence-associated genes on this plasmid. By BLASTn search on the NCBI database, pWWTP-03 showed 100% query coverage and 99.2% identity to another plasmid pCD74–2–2 (Figure S4), which was also an Inc.X1 plasmid with 44.7 kb length . pCD74–2–2 was isolated from retail chicken meat as well as human food.…”

Section: Resultsmentioning

confidence: 99%

Deciphering Multidrug-Resistant Plasmids in Disinfection Residual Bacteria from a Wastewater Treatment Plant

Lin,

Zhou,

Shuai

et al. 2024

Environ. Sci. Technol.

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Current disinfection processes pose an emerging environmental risk due to the ineffective removal of antibioticresistant bacteria, especially disinfection residual bacteria (DRB) carrying multidrug-resistant plasmids (MRPs). However, the characteristics of DRB-carried MRPs are poorly understood. In this study, qPCR analysis reveals that the total absolute abundance of four plasmids in postdisinfection effluent decreases by 1.15 log units, while their relative abundance increases by 0.11 copies/cell compared to investigated wastewater treatment plant (WWTP) influent. We obtain three distinctive DRB-carried MRPs (pWWTP-01−03) from postdisinfection effluent, each carrying 9−11 antibiotic-resistant genes (ARGs). pWWTP-01 contains all 11 ARGs within an ∼25 Kbp chimeric genomic island showing strong patterns of recombination with MRPs from foodborne outbreaks and hospitals. Antibiotic-, disinfectant-, and heavy-metal-resistant genes on the same plasmid underscore the potential roles of disinfectants and heavy metals in the coselection of ARGs. Additionally, pWWTP-02 harbors an adhesin-type virulence operon, implying risks of both antibiotic resistance and pathogenicity upon entering environments. Furthermore, some MRPs from DRB are capable of transferring and could confer selective advantages to recipients under environmentally relevant antibiotic pressure. Overall, this study advances our understanding of DRB-carried MRPs and highlights the imminent need to monitor and control wastewater MRPs for environmental security.

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Distribution of virulence factors, antimicrobial resistance genes and phylogenetic relatedness among Shiga toxin-producing Escherichia coli serogroup O91 from human infections

Nüesch‐Inderbinen

Stevens

Cernela

et al. 2021

International Journal of Medical Microbiology

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Identification of Novel Plasmids Containing the Tigecycline Resistance Gene tet(X4) in Escherichia coli Isolated from Retail Chicken Meat

Cited by 5 publications

References 10 publications

Dissemination and prevalence of plasmid-mediated high-level tigecycline resistance gene tet (X4)

Dissemination and prevalence of plasmid-mediated high-level tigecycline resistance gene tet (X4)

Deciphering Multidrug-Resistant Plasmids in Disinfection Residual Bacteria from a Wastewater Treatment Plant

Distribution of virulence factors, antimicrobial resistance genes and phylogenetic relatedness among Shiga toxin-producing Escherichia coli serogroup O91 from human infections

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