Identification of novel molecular candidates for fatty liver in the hyperlipidemic mouse model, HcB19

Greevenbroek, Marleen M.J. van; Vermeulen, Vicky M. M.-J.; Bruin, T.W.A. de

doi:10.1194/jlr.m400062-jlr200

Cited by 24 publications

(15 citation statements)

References 35 publications

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“…In addition to these enzymes involved in methylation, the oxidoreductase glyco‐enzyme 3‐HAO was repressed in 3–month‐old rats after intrauterine exposure to alcohol. This enzyme participates in tryptophan metabolism leading to quinolate formation and NAD biosynthesis and has been suggested to be important in the development of fatty liver and hyperlipidemia 65, 66. Interestingly, we have shown that rats exposed to alcohol in utero are hyperlipidemic and develop fatty liver as adults 4.…”

Section: Discussionmentioning

confidence: 68%

Prenatal alcohol exposure alters phosphorylation and glycosylation of proteins in rat offspring liver

Fofana¹,

Yao²,

Rampitsch³

et al. 2010

Proteomics

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To gain more insights into the translational and PTM that occur in rat offspring exposed to alcohol in utero, 2-D PAGE with total, phospho- and glycoprotein staining and MALDI-MS/MS and database searching were conducted. The results, based on fold-change expression, revealed a down-regulation of total protein expression by prenatal alcohol exposure in 7-day-old and 3-month-old rats. There was an up-regulation of protein phosphorylation but a down-regulation of glycosylation by prenatal alcohol exposure in both age groups. Of 31 protein spots examined per group, differentially expressed proteins were identified as ferritin light chain, aldo-keto reductase, tumor rejection antigen gp96, fructose-1,6-bisphosphatase, glycerol-3-phosphate dehydrogenase, malate dehydrogenase, and gamma-actin. Increased phosphorylation was observed in proteins such as calmodulin, gluthatione S-transferase, glucose regulated protein 58, alpha-enolase, eukaryotic translation elongation factor 1 beta-2, riboprotein large P2, agmatinase, ornithine carbamoyltransferase, quinolinate phosphoribosyltransferase, formimidoyltransferase cyclodeaminase, and actin. In addition, glycosylation of adenosine kinase, adenosylhomocysteine hydrolase, and 3-hydroxyanthranilate dioxygenase was reduced. Pathways affected by these protein alterations include cell signaling, cellular stress, protein synthesis, cytoskeleton, as well as glucose, aminoacid, adenosine and energy metabolism. The activity of the gluconeogenic enzyme fructose-1,6-bisphosphatase was elevated by prenatal alcohol. The observations may have important physiological implications.

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Section: Discussionmentioning

confidence: 68%

Prenatal alcohol exposure alters phosphorylation and glycosylation of proteins in rat offspring liver

Fofana¹,

Yao²,

Rampitsch³

et al. 2010

Proteomics

View full text Add to dashboard Cite

show abstract

“…The expression level of glycerol-3-phosphate dehydrogenase increased in diet-induced obese animals [17]. Propionyl-coenzyme A-carboxylase is the key enzyme in the catabolism of odd-chain fatty acids, isoleucine, threonine, methionine and valine [18]. Phosphatidylinositol is an important lipid, both as a key membrane constituent and as a participant in essential signaling processes in all plants and animals.…”

Section: Discussionmentioning

confidence: 99%

Profiling of chicken adipose tissue gene expression by genome array

Wang

et al. 2007

BMC Genomics

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Background: Excessive accumulation of lipids in the adipose tissue is a major problem in the present-day broiler industry. However, few studies have analyzed the expression of adipose tissue genes that are involved in pathways and mechanisms leading to adiposity in chickens. Gene expression profiling of chicken adipose tissue could provide key information about the ontogenesis of fatness and clarify the molecular mechanisms underlying obesity. In this study, Chicken Genome Arrays were used to construct an adipose tissue gene expression profile of 7-week-old broilers, and to screen adipose tissue genes that are differentially expressed in lean and fat lines divergently selected over eight generations for high and low abdominal fat weight.

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“…Lumiglo chemiluminescence (KPL) was used to detect bound protein, and the protein was quantified using QuantityOne® analysis software (Bio-Rad). These standard methods have been routinely used by other investigators (Chatagnon et al, 2009;van Greevenbroek, Vermeulen, & de Bruin, 2004). For the Western blot analysis of p-eNOS (ser 1177), goat polyclonal IgG obtained from Santa Cruz Biotechnology, (Santa Cruz, CA) was diluted 1:1000 for incubation overnight.…”

Section: Western Blot Analysismentioning

confidence: 99%

Effects of insulin detemir on balloon catheter injured carotid artery in Zucker fatty rats

Murthy

Pankey

Banka

et al. 2012

Journal of Diabetes and its Complications

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Identification of novel molecular candidates for fatty liver in the hyperlipidemic mouse model, HcB19

Cited by 24 publications

References 35 publications

Prenatal alcohol exposure alters phosphorylation and glycosylation of proteins in rat offspring liver

Prenatal alcohol exposure alters phosphorylation and glycosylation of proteins in rat offspring liver

Profiling of chicken adipose tissue gene expression by genome array

Effects of insulin detemir on balloon catheter injured carotid artery in Zucker fatty rats

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