Identification of novel long chain <i>N</i>-acylhomoserine lactones of chain length C20 from the marine phototrophic bacterium <i>Rhodovulum sulfidophilum</i>

Arashida, Naoko; Shimbo, Kazutaka; Terada, T.; Okimi, Takuya; Kikuchi, Yo; Hashiro, Shuhei; Umekage, So; Yasueda, Hisashi

doi:10.1080/09168451.2018.1490168

Cited by 10 publications

(6 citation statements)

References 32 publications

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“…N -acyl homoserine lactones (AHLs) are the best characterized QS signal molecules. Different AHLs possess a homoserine lactone ring with an attached fatty acyl side chain of 4 to 20 carbons 13,14 . Detection of AHLs occurs either directly by LuxR regulators within the cell, or by membrane-bound two-component histidine kinase-type proteins 15,16 .…”

Section: Introductionmentioning

confidence: 99%

Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones

Malešević

Lorenzo

Filipić

et al. 2019

Sci Rep

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Pseudomonas aeruginosa is one of the most common opportunistic pathogens that use quorum sensing (QS) system to regulate virulence factors expression and biofilm development. Delftia sp. 11304 was selected among 663 Gram-negative clinical isolates based on its QS inhibitory activity against P. aeruginosa MMA83 clinical isolate. Whole genome sequencing identified this isolate as D. tsuruhatensis and revealed genetic armamentarium of virulence factors and antibiotic resistance determinants. Ethyl acetate extract of D. tsuruhatensis 11304 culture supernatant (QSI extract) prevented biofilm formation of P. aeruginosa MMA83, but was unable to cause biofilm decomposition. QSI extract showed a synergistic effect in combination with meropenem and gentamycin, against P. aeruginosa MMA83. A dose-dependent reduction of the virulence factors: elastase, rhamnolipid and pyocyanin production by P. aeruginosa MMA83 and significant downregulation of lasI, lasR, rhlI, rhlR, pqs and mvfR expression were observed. Matrix-assisted Laser Desorption Ionization (MALDI) mass spectrometry of D. tsuruhatensis 11304 QSI extract revealed the presence of N-acyl homoserine lactones (AHL) with chain lengths of C12 to C18. The main ion peak was identified as N-octadecanoylhomoserine lactone (C18-HSL). Commercial C18-HSL (20 µM) reduced pyocyanin production as well as mRNA level of the lasI gene. A novel AHL species, dihydroxy-N-octadecanoylhomoserine lactone, was also described.

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Section: Introductionmentioning

confidence: 99%

Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones

Malešević

Lorenzo

Filipić

et al. 2019

Sci Rep

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“…AHLs vary in the number of carbons in the acyl chain (C4–C20), in substitutions at C-3 (hydrogen, hydroxy, and oxo groups), and in the degrees of unsaturation in the acyl chain. Among these, longer chain-length AHLs are common among marine bacteria, and an acyl chain length of 20 carbons was recently reported in the marine bacterium Rhodovulum sulfidophilum . , AHLs have been previously extensively studied in terrestrial bacteria, but a limited knowledge base exists regarding AHL production and their biological roles in marine bacteria except for shorter chain-length AHLs in Vibrio spp.…”

Section: Resultsmentioning

confidence: 87%

Metabolomics Approaches to Dereplicate Natural Products from Coral-Derived Bioactive Bacteria

et al. 2022

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Stony corals (Scleractinia) are invertebrates that form symbiotic relationships with eukaryotic algal endosymbionts and the prokaryotic microbiome. The microbiome has the potential to produce bioactive natural products providing defense and resilience to the coral host against pathogenic microorganisms, but this potential has not been extensively explored. Bacterial pathogens can pose a significant threat to corals, with some species implicated in primary and opportunistic infections of various corals. In response, probiotics have been proposed as a potential strategy to protect corals in the face of increased incidence of disease outbreaks. In this study, we screened bacterial isolates from healthy and diseased corals for antibacterial activity. The bioactive extracts were analyzed using untargeted metabolomics. Herein, an UpSet plot and hierarchical clustering analyses were performed to identify isolates with the largest number of unique metabolites. These isolates also displayed different antibacterial activities. Through application of in silico and experimental approaches coupled with genome analysis, we dereplicated natural products from these coral-derived bacteria from Florida's coral reef environments. The metabolomics approach highlighted in this study serves as a useful resource to select probiotic candidates and enables insights into natural product-mediated chemical ecology in holobiont symbiosis.

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“…However, a large number of natural products have been isolated in Actinobacteria [ 23 , 35 ], and more than half of the KS genes in the Uniprot database belong to Actinobacteria [ 36 , 37 ]. The genus Streptomyces continues to be the predominant source of new chemistries, with 167 new metabolites reported during 2018, representing >69% of the marine-sourced bacterial natural products [ 38 ], although in recent years marine bioactives have been reported in other species, such as Roseovarius tolerans [ 39 ], Rhodovulum sulfidophilum [ 40 ], and Aequorivita sp. [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Deep-Sea Sediments from the Southern Gulf of Mexico Harbor a Wide Diversity of PKS I Genes

et al. 2022

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The excessive use of antibiotics has triggered the appearance of new resistant strains, which is why great interest has been taken in the search for new bioactive compounds capable of overcoming this emergency in recent years. Massive sequencing tools have enabled the detection of new microorganisms that cannot be cultured in a laboratory, thus opening the door to the search for new biosynthetic genes. The great variety in oceanic environments in terms of pressure, salinity, temperature, and nutrients enables marine microorganisms to develop unique biochemical and physiological properties for their survival, enhancing the production of secondary metabolites that can vary from those produced by terrestrial microorganisms. We performed a search for type I PKS genes in metagenomes obtained from the marine sediments of the deep waters of the Gulf of Mexico using Hidden Markov Models. More than 2000 candidate genes were detected in the metagenomes that code for type I PKS domains, while biosynthetic pathways that may code for other secondary metabolites were also detected. Our research demonstrates the great potential use of the marine sediments of the Gulf of Mexico for identifying genes that code for new secondary metabolites.

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Identification of novel long chain N-acylhomoserine lactones of chain length C20 from the marine phototrophic bacterium Rhodovulum sulfidophilum

Cited by 10 publications

References 32 publications

Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones

Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones

Metabolomics Approaches to Dereplicate Natural Products from Coral-Derived Bioactive Bacteria

Deep-Sea Sediments from the Southern Gulf of Mexico Harbor a Wide Diversity of PKS I Genes

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