Identification of Novel Locus RsCr6 Related to Clubroot Resistance in Radish (Raphanus sativus L.)

Abstract

Clubroot is a devastating disease that causes substantial yield loss worldwide. However, the inheritance and molecular mechanisms of clubroot resistance during pathogen infection in radish remain largely unclear. In this study, we investigated the inheritance of clubroot resistance in the F2 population derived from crossing clubroot-resistant (CR) and clubroot-susceptible inbred lines “GLX” and “XNQ,” respectively. Genetic analysis revealed that a single dominant gene controlled the clubroot resistance of “GLX” with a Mendelian ratio of resistance and susceptibility of nearly 3:1. Bulked segregant analysis combined with whole-genome resequencing (BSA-seq) was performed to detect the target region of RsCr6 on chromosome Rs8. Linkage analysis revealed that the RsCr6 locus was located between two markers, HB321 and HB331, with an interval of approximately 92 kb. Based on the outcomes of transcriptome analysis, in the RsCr6 locus, the R120263140 and R120263070 genes with a possible relation to clubroot resistance were considered candidate genes. In addition, three core breeding materials containing the two reported quantitative trait loci (QTLs) and our novel locus RsCr6 targeting clubroot resistance were obtained using marker-assisted selection (MAS) technology. This study reveals a novel locus responsible for clubroot resistance in radishes. Further analysis of new genes may reveal the molecular mechanisms underlying the clubroot resistance of plants and provide a theoretical basis for radish resistance breeding.

Advances in Biological Control and Resistance Genes of Brassicaceae Clubroot Disease-The Study Case of China

“…Gan et al identified five QTL loci, RsCr1, RsCr2, RsCr3, RsCr4, and RsCr5, associated with clubroot disease resistance, with RsCr1 being homologous to the well-defined locus Crr1 [107]. Recently, Gan et al identified a new locus RsCr6 on chromosome 8 and screened for possible resistance candidate genes R120263140 and R120263070 [108].…”

Functions of the AP2/ERF family transcription factor AIL7 in immunity against soilborne clubroot pathogen in Arabidopsis

“…Soilborne plant pathogens can be highly devastating in terms of crop health, yield, and economic sustainability, and the chemical strategies currently deployed to combat these pathogens have often proven inadequate (Delgado-Baquerizo et al, 2020). Clubroot, for example, is one of the most destructive soilborne diseases of plants in the Brassicaceae family, which provides the second-largest source of vegetable oil and third largest source of vegetables, including rapeseed, mustard, radish, cabbage, kohlrabi, cauliflower, and broccoli (Gan et al, 2022; Hasan et al, 2021; FAO 2020). This disease is caused by an obligate biotrophic protist, Plasmodiophora brassicae , which can survive in the soil as long-lived resting spores.…”

Identification of Clubroot-Resistant Germplasm in a Radish (Raphanus sativus L.) Core Collection

“…Clubroot disease is a rapidly increasing and serious destructive factor affecting the yield and quality of radishes. In China, radish Agronomy 2024, 14, 157 2 of 12 clubroot disease occurs in provinces including Yunnan, Sichuan, Hubei, Henan, etc., to varying degrees annually, causing serious economic losses [10,11].…”