Identification of novel immunogenic proteins in pathogenic Haemophilus parasuis based on genome sequence analysis

Hong, Min-Hee; Ahn, Jungoh; Yoo, Sung-Sik; Hong, Jung Hwa; Lee, Eungyu; Yoon, In-Joong; Jung, Joon-Ki; Lee, Hongweon

doi:10.1016/j.vetmic.2010.07.023

Cited by 28 publications

(15 citation statements)

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“…CsgG had previously been observed to be up-regulated in vivo in pig lung during the acute phase of disease and during biofilm formation [11], [22]. Interestingly, the curli protein assembly was identified as a potent immunogenic protein in Haemophilus parasuis , the cause of Glässer's disease in pigs [28]. The elongation factor tufB , has also previously been identified as a potential virulence factor, and is possibly involved in fibronectin binding [18].…”

Section: Resultsmentioning

confidence: 99%

Transcriptional Portrait of Actinobacillus pleuropneumoniae during Acute Disease - Potential Strategies for Survival and Persistence in the Host

et al. 2012

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BackgroundGene expression profiles of bacteria in their natural hosts can provide novel insight into the host-pathogen interactions and molecular determinants of bacterial infections. In the present study, the transcriptional profile of the porcine lung pathogen Actinobacillus pleuropneumoniae was monitored during the acute phase of infection in its natural host.Methodology/Principal FindingsBacterial expression profiles of A. pleuropneumoniae isolated from lung lesions of 25 infected pigs were compared in samples taken 6, 12, 24 and 48 hours post experimental challenge. Within 6 hours, focal, fibrino hemorrhagic lesions could be observed in the pig lungs, indicating that A. pleuropneumoniae had managed to establish itself successfully in the host. We identified 237 differentially regulated genes likely to encode functions required by the bacteria for colonization and survival in the host. This group was dominated by genes involved in various aspects of energy metabolism, especially anaerobic respiration and carbohydrate metabolism. Remodeling of the bacterial envelope and modifications of posttranslational processing of proteins also appeared to be of importance during early infection. The results suggested that A. pleuropneumoniae is using various strategies to increase its fitness, such as applying Na+ pumps as an alternative way of gaining energy. Furthermore, the transcriptional data provided potential clues as to how A. pleuropneumoniae is able to circumvent host immune factors and survive within the hostile environment of host macrophages. This persistence within macrophages may be related to urease activity, mobilization of various stress responses and active evasion of the host defenses by cell surface sialylation.Conclusions/SignificanceThe data presented here highlight the importance of metabolic adjustments to host conditions as virulence factors of infecting microorganisms and help to provide insight into the mechanisms behind the efficient colonization and persistence of A. pleuropneumoniae during acute disease.

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Section: Resultsmentioning

confidence: 99%

Transcriptional Portrait of Actinobacillus pleuropneumoniae during Acute Disease - Potential Strategies for Survival and Persistence in the Host

et al. 2012

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“…The lack of effective vaccines against a broad spectrum of strains has limited disease control. With the development of modern vaccines based on molecular techniques, subunit vaccines have attracted more attention, with particular interest in the identification of proteinbased vaccine candidates (10)(11)(12). It has been shown that recombinant vaccines based on outer membrane proteins (OMPs) provided partial protection against a challenge with H. parasuis (13).…”

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confidence: 99%

Cross-Protective Efficacy of Recombinant Transferrin-Binding Protein A of Haemophilus parasuis in Guinea Pigs

Huang

et al. 2013

Clin Vaccine Immunol

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bThe causative agent of Glasser's disease in swine is Haemophilus parasuis. Commercial bacterins are widely used for protection of the swine population. However, cross protection is limited because H. parasuis has more than 15 serovars. Transferrin-binding protein A has shown potential as a broad-spectrum vaccine candidate against homologous and heterologous strains. Here we amplified the full-length tbpA gene from an H. parasuis serovar 13 isolate and cloned it into a pET-SUMO expression vector. We then expressed and purified the TbpA protein by Ni affinity chromatography. First, the immunogenicity and protective efficacy of the protein were evaluated in guinea pigs by two subcutaneous immunizations with different doses of Montanide IMS 206 VG adjuvant. The immunized guinea pigs were, respectively, challenged on week 3 after a booster immunization with homologous strain LJ3 (serovar 13) and heterologous strain FX1 (serovar 4), and vaccine-inoculated groups were compared with nonvaccinated controls. All immunized groups showed serum antibody titers higher than those of negative-control groups. Furthermore, the cytokine and chemokine levels were evaluated at the transcriptional level by the real-time PCR analysis of six cytokines and chemokines. Gamma interferon and interleukin-5 in groups immunized with 100 g were elevated more than 15-fold over those in negative-control groups. The protection rates were 80 and 60% after a challenge with strains LJ3 and FX1, respectively, in the groups vaccinated with 100 g of recombinant TbpA protein. Subsequently, the data showed that guinea pigs immunized with a single dose (100 g) were protected at levels of 80, 80, and 60% against LJ3, FX1, and another heterologous strain, SZ (serovar 14), respectively. The results indicate for the first time that TbpA protein cross protects guinea pigs against serovars 13, 4, and 14 of H. parasuis. Taken together, these results suggest that the recombinant TbpA protein is a promising vaccine candidate that needs to be confirmed in a swine population.

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“…Because of the interference in the development of active immunity by vaccine, the period of application is an important issue. Reverse vaccinology and immunoproteomic analysis identified several putative virulence-associated genes and immunogenic proteins in different H. parasuis strains (Hong et al 2011). In order to avoid the development of clinical signs or lesions characteristic of systemic infection after be challenged, it is recommended to vaccinated at least sows.…”

Section: How Prevent Problems With H Parasuis In Pig Productionmentioning

confidence: 99%

The challenges with Glässer’s disease in technified pig production

Pereira

Dalla-Costa

Ferroni

et al. 2017

Austral j. vet. sci.

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ABSTRACT. The swine upper respiratory tract is early colonised by Haemophilus parasuis, a bacteria which causes Glässer´s disease under favorable conditions. Glässer's disease is a septicemic infectious disease characterised by causing polyserositis. The prevention of Glässer disease still represents a big challenge for the production chain, since the mechanism of systemic infection in pigs and virulence factors that prevent phagocytosis are not yet well understood. Even in swine herds with high sanitary standard, it is the main cause of mortality that has led to productive and economic losses in the pig industry worldwide. Although the H. parasuis genome sequence has been completed already, diagnosis is still difficult due to the existence of non-virulent strains and the early colonisation of the upper respiratory tract of healthy swines. This review aims to provide up-to-date information about the etiology, epidemiology, pathogenesis, clinical signs, gross and microscopic lesions, diagnosis, treatment and control of Glässer's disease.Key words: epidemiology, swine, virulence factors, vaccines. RESUMEN.El tracto respiratorio superior del cerdo es colonizado inicialmente por Haemophilus parasuis, bacteria que en condiciones favorables causa la enfermedad de Glässer. La enfermedad de Glässer es una afección infecciosa que se caracteriza por el desarrollo de poliserositis septicémica. La prevención de esta enfermedad sigue siendo un problema en la producción porcina tecnificada, ya que los mecanismos de virulencia de este microorganismo y los factores sistémicos que impiden la fagocitosis no son bien conocidos. Inclusive en rebaños con un alto estándar de salud, H. parasuis es la principal causa de mortalidad, generando así pérdidas productivas y económicas en la industria porcina a nivel mundial. A pesar de que la secuencia del genoma de H. parasuis se ha completado recientemente, el diagnóstico aún se complica por la existencia de cepas no virulentas y la temprana colonización del tracto respiratorio superior de cerdos sanos. El objetivo de esta revisión es entregar información actualizada respecto de la etiología, epidemiología, patogénesis, signos clínicos, lesiones macroscópicas y microscópicas, diagnóstico, tratamiento y control de la enfermedad de Glässer.

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Identification of novel immunogenic proteins in pathogenic Haemophilus parasuis based on genome sequence analysis

Cited by 28 publications

References 12 publications

Transcriptional Portrait of Actinobacillus pleuropneumoniae during Acute Disease - Potential Strategies for Survival and Persistence in the Host

Transcriptional Portrait of Actinobacillus pleuropneumoniae during Acute Disease - Potential Strategies for Survival and Persistence in the Host

Cross-Protective Efficacy of Recombinant Transferrin-Binding Protein A of Haemophilus parasuis in Guinea Pigs

The challenges with Glässer’s disease in technified pig production

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Identification of novel immunogenic proteins in pathogenic Haemophilus parasuis based on genome sequence analysis

Cited by 28 publications

References 12 publications

Transcriptional Portrait of Actinobacillus pleuropneumoniae during Acute Disease - Potential Strategies for Survival and Persistence in the Host

Transcriptional Portrait of Actinobacillus pleuropneumoniae during Acute Disease - Potential Strategies for Survival and Persistence in the Host

Cross-Protective Efficacy of Recombinant Transferrin-Binding Protein A of Haemophilus parasuis in Guinea Pigs

The challenges with Glässer&#8217;s disease in technified pig production

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The challenges with Glässer’s disease in technified pig production