Identification of novel immunogenic proteins of Helicobacter pylori by proteome technology

Utt, Meeme; Nilsson, Ingrid; Ljungh, Åsa; Wadström, Torkel

doi:10.1016/s0022-1759(01)00476-8

Cited by 46 publications

(33 citation statements)

References 26 publications

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“…Proteome technology using 2-DE gel by ESI-MS-MS has been used to identify four H. pylori immunogenic low-molecular-weight (25-30 KDa) proteins: UreA, cell binding factor 2, hypothetical protein and outer membrane protein. The specificity of these proteins was evaluated using both 1-D and 2-D immunoblotting against a group of sera from patients with different bacterial infections (Utt et al, 2002). Thus far, there are no reports of 2-DE antigenic protein analysis of a Malaysian H. pylori isolate.…”

Section: Discussionmentioning

confidence: 99%

Antigenic Proteins of Helicobacter pylori of Potential Diagnostic Value

Khalilpour

Agilan²,

Lee³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Helicobacter pylori antigen was prepared from an isolate from a patient with a duodenal ulcer. Serum samples were obtained from culture-positive H. pylori infected patients with duodenal ulcers, gastric ulcers and gastritis (n=30). As controls, three kinds of sera without detectable H. pylori IgG antibodies were used: 30 from healthy individuals without history of gastric disorders, 30 from patients who were seen in the endoscopy clinic but were H. pylori culture negative and 30 from people with other diseases. OFF-GEL electrophoresis, SDS-PAGE and Western blots of individual serum samples were used to identify protein bands with good sensitivity and specificity when probed with the above sera and HRP-conjugated anti-human IgG. Four H. pylori protein bands showed good (≥ 70%) sensitivity and high specificity (98-100%) towards anti-Helicobacter IgG antibody in culturepositive patients sera and control sera, respectively. The identities of the antigenic proteins were elucidated by mass spectrometry. The relative molecular weights and the identities of the proteins, based on MALDI TOF/ TOF, were as follows: CagI (25 kDa), urease G accessory protein (25 kDa), UreB (63 kDa) and proline/pyrroline-5-carboxylate dehydrogenase (118 KDa). These identified proteins, singly and/or in combinations, may be useful for diagnosis of H. pylori infection in patients.

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Section: Discussionmentioning

confidence: 99%

Antigenic Proteins of Helicobacter pylori of Potential Diagnostic Value

Khalilpour

Agilan²,

Lee³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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“…Gel-based immunoproteomic approaches have proven successful in the identification of highly specific H. pylori antigens that are useful for the improvement of serological tests to diagnose and monitor H. pylori infection, and thus to identify individuals who are at risk for gastric cancer [83,84]. In addition, H. pylori is also an important risk factor of duodenal ulcer.…”

Section: Gastric Cancermentioning

confidence: 99%

Immunoproteomics: From biomarker discovery to diagnostic applications

Tjalsma

Schaeps

Swinkels

2008

Proteomics Clinical Apps

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Circulating antibodies reflect a molecular imprint of antigens that are related to autoimmune diseases, cancer or infection. Importantly, serum antibodies are useful clinical markers as they carry diagnostic information from all around the human body. Moreover, the amplification cascade governed by the humoral immune system causes a surplus of circulating antibodies after appearance of the corresponding (low abundance) antigen. In combination with the fact that antibodies are highly stable compared to many other serum proteins, they seem ideal to be implemented in clinical diagnostic assays for the detection of antigen-associated diseases. This review summarises advances in immunoproteomics with respect to technologies for biomarker discovery, with special emphasis on recently developed gel-free MS-based approaches, and looks forward to potential immunoproteomic applications in diagnostic medicine.

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“…Additional high throughput methods to investigate the bidirectional relationship include proteomics and functional genomic technologies such as RNA interference (46). Some promising results from investigations using proteomics to uncover novel immunogenic proteins have been reported (47,48). These studies might enhance understanding of disease pathogenesis and facilitate the development of effective biomarkers for diagnosis, prevention, and treatment.…”

Section: Technological Advances Have Shed Light On Investigations Of mentioning

confidence: 99%

Host-Environment Interactions: Their Impact on Progression from Gastric Inflammation to Carcinogenesis and on Development of New Approaches to Prevent and Treat Gastric Cancer

Wu¹,

Chen

Lin³

2005

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Revelation of the connection between Helicobacter pylori infection and gastric adenocarcinoma has prompted new investigations pertaining to its basic and clinical aspects. H. pylori -induced persistent and uncontrolled gastric inflammation nearly always precedes the development of cancer and is instrumental in initiating a multistep process leading to carcinogenesis. Despite initial optimism about the potential of combination anti -H. pylori therapy to ultimately eradicate gastric adenocarcinoma, recent investigations suggest its use should be targeted and tailored to a selected patient group considering the multifaceted role of H. pylori in disease and the disease heterogeneity of gastric adenocarcinoma. The clinical spectrum of H. pylori infection ranges from asymptomatic gastritis and peptic ulcer to gastric malignancies. The occurrence of one versus another is the result of differences in the magnitude of gastritis, and the current disease paradigm suggests gastric inflammation is common to all H. pylori -associated gastroduodenal diseases. Therefore, the host inflammatory responses to environmental triggers, rather than to bacteria or environmental factors per se, would dictate the variable outcomes of H. pylori infection. Putative factors that are expected to play an important role in stimulating inflammatory pathways and modulating the cross-talk between host and environment are age at the time of infection, environmental cofactors, H. pylori virulence, and host genetics. Elucidation of the intimate relationship between host-environment interaction and gastric inflammation, although currently a formidable task, is essential in the development of new prevention and treatment strategies. Such knowledge might provide clues that allow more accurate prediction of variable outcomes of gastric inflammation and appropriate adjustment of treatment strategies, and might open up novel areas for studying gastric carcinogenesis. The evolving new technologies, such as microarray, proteomic, and functional genomic analyses, promise to shed new light on the immense complexity of the presumed host-environment interactions and will reveal more useful markers for the diagnosis and prognosis of gastric adenocarcinoma. (Cancer Epidemiol Biomarkers Prev 2005;14(8):1878 -82)

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Identification of novel immunogenic proteins of Helicobacter pylori by proteome technology

Cited by 46 publications

References 26 publications

Antigenic Proteins of Helicobacter pylori of Potential Diagnostic Value

Antigenic Proteins of Helicobacter pylori of Potential Diagnostic Value

Immunoproteomics: From biomarker discovery to diagnostic applications

Host-Environment Interactions: Their Impact on Progression from Gastric Inflammation to Carcinogenesis and on Development of New Approaches to Prevent and Treat Gastric Cancer

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