Identification of Novel Hub Genes Associated With Gastric Cancer Using Integrated Bioinformatics Analysis

Abstract

Background: Gastric cancer (GC) is one of the most common solid malignant tumors worldwide with a high-recurrence-rate. Identifying the molecular signatures and specific biomarkers of GC might provide novel clues for GC prognosis and targeted therapy.Methods: Gene expression profiles were obtained from the ArrayExpress and Gene Expression Omnibus database. Differentially expressed genes (DEGs) were picked out by R software. The hub genes were screened by cytoHubba plugin. Their prognostic values were assessed by Kaplan–Meier survival analyses and the gene expression profiling interactive analysis (GEPIA). Finally, qRT-PCR in GC tissue samples was established to validate these DEGs. Results: Total of 295 DEGs were identified between GC and their corresponding normal adjacent tissue samples in E-MTAB-1440, GSE79973, GSE19826, GSE13911, GSE27342, GSE33335 and GSE56807 datasets, including 117 up-regulated and 178 down-regulated genes. Among them, 7 vital upregulated genes (HMMR, SPP1, FN1, CCNB1, CXCL8, MAD2L1 and CCNA2) were selected. Most of them had a significantly worse prognosis except SPP1. Using qRT-PCR, we validated that their transcriptions in our GC tumor tissue were upregulated except SPP1 and FN1, which correlated with tumor relapse and predicts poorer prognosis in GC patients.Discussion: We have identified 5 upregulated DEGs (HMMR, CCNB1, CXCL8, MAD2L1, and CCNA2) in GC patients with poor prognosis using integrated bioinformatical methods, which could be potential biomarkers and therapeutic targets for GC treatment.

Specific Blood RNA Profiles in Individuals with Acute Spinal Cord Injury as Compared with Trauma Controls

“…It stimulates the formation of tubulin polymers and results in tubulin fragments being formed at microtubule ends [29]. CEP55, HMMR, CDKN3, and CDC25C are essential for cell cycle progression [30][31][32][33]. PRTN3 is a serine protease found on 9 Oxidative Medicine and Cellular Longevity neutrophils and monocytes and binds to the surface of the endothelium but can also be internalized which induces apoptosis [34].…”

RHAMM Is a Multifunctional Protein That Regulates Cancer Progression

“…These show that elevated RHAMM expression is characteristic of most cancers and prognostic of a poor outcome in many. For example, elevated RHAMM expression is an indicator of poor prognosis in breast cancer [ 119 , 120 , 121 ], multiple myeloma [ 122 ], oral squamous cell carcinoma [ 123 ], ovarian cancer [ 124 ], lung cancer [ 125 ], prostate cancer [ 126 ], colon cancer [ 127 ], and gastric cancer [ 128 ]. However, histology analyses of these human tumors reveal that RHAMM expression is heterogeneous, and high RHAMM expression is often limited to a small subset of cancer cells [ 119 ].…”